The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Quantitative Chemical Imaging with Multiplex Stimulated Raman Scattering Microscopy
Open accessAbstract
Stimulated Raman scattering (SRS) microscopy is a newly developed label-free chemical imaging technique that overcomes the speed limitation of confocal Raman microscopy while avoiding the nonresonant background problem of coherent anti-Stokes Raman scattering (CARS) microscopy. Previous demonstrations have been limited to single Raman band measurements. We present a novel modulation multiplexing approach that allows real-time detection of multiple species using the fast Fourier transform. We demonstrate the quantitative determination of chemical concentrations in a ternary mixture. Furthermore, two imaging applications are pursued: (1) quantitative determination of oil content as well as pigment and protein concentration in microalgae cultures; and (2) 3D high-resolution imaging of blood, lipids, and protein distribution in ex vivo mouse skin tissue. We believe that quantitative multiplex SRS uniquely combines the advantage of fast label-free imaging with the fingerprinting capability of Raman spectroscopy and enables numerous applications in lipid biology as well as biomedical imaging.
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Cites
Microalgae for biodiesel production and other applications: A review
Mata, T. M., Martins, A. A., & Caetano, N. S. (2010) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2009.07.020 · Cited by 5,685
Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances
Hu, Q., Sommerfeld, M., Jarvis, E., Ghirardi, M., Posewitz, M., Seibert, M., & Darzins, A. (2008) · The Plant Journal · doi:10.1111/j.1365-313x.2008.03492.x · Cited by 3,745
Label-Free Biomedical Imaging with High Sensitivity by Stimulated Raman Scattering Microscopy
Freudiger, C. W., Min, W., Saar, B. G., Lu, S., Holtom, G. R., He, C., Tsai, J. C., Kang, J. X., & Xie, X. S. (2008) · Science · doi:10.1126/science.1165758 · Cited by 2,255
Self-citationCoherent Anti-Stokes Raman Scattering Microscopy: Chemical Imaging for Biology and Medicine
Evans, C. L., & Xie, X. S. (2008) · Annual Review of Analytical Chemistry · doi:10.1146/annurev.anchem.1.031207.112754 · Cited by 1,150
Self-citationLi, Y., Horsman, M., Wu, N., Lan, C. Q., & Dubois‐Calero, N. (2008) · Biotechnology Progress · doi:10.1021/bp070371k · Cited by 1,134
Coherent Anti-Stokes Raman Scattering Microscopy: Instrumentation, Theory, and Applications
Cheng, J.-X., & Xie, X. S. (2004) · The Journal of Physical Chemistry B · doi:10.1021/jp035693v · Cited by 963
Self-citationVideo-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering
Saar, B. G., Freudiger, C. W., Reichman, J., Stanley, C. M., Holtom, G. R., & Xie, X. S. (2010) · Science · doi:10.1126/science.1197236 · Cited by 844
Self-citationBotryococcus braunii : A Renewable Source of Hydrocarbons and Other Chemicals
Banerjee, A., Sharma, R., Chisti, Y., & Banerjee, U. C. (2002) · Critical Reviews in Biotechnology · doi:10.1080/07388550290789513 · Cited by 714
In vivo lipidomics using single-cell Raman spectroscopy
Wu, H., Volponi, J. V., Oliver, A. E., Parikh, A. N., Simmons, B. A., & Singh, S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1009043108 · Cited by 436
Vibrational imaging based on stimulated Raman scattering microscopy
Nandakumar, P., Kovalev, A., & Volkmer, A. (2009) · New Journal of Physics · doi:10.1088/1367-2630/11/3/033026 · Cited by 401
Cited by
Vibrational spectroscopic imaging of living systems: An emerging platform for biology and medicine
Cheng, J.-X., & Xie, X. S. (2015) · Science · doi:10.1126/science.aaa8870 · Cited by 874
Self-citationHigh-speed coherent Raman fingerprint imaging of biological tissues
Camp Jr, C. H., Lee, Y. J., Heddleston, J. M., Hartshorn, C. M., Walker, A. R. H., Rich, J. N., Lathia, J. D., & Cicerone, M. T. (2014) · Nature Photonics · doi:10.1038/nphoton.2014.145 · Cited by 469
Raman spectroscopy: techniques and applications in the life sciences
Shipp, D. W., Sinjab, F., & Notingher, I. (2017) · Advances in Optics and Photonics · doi:10.1364/aop.9.000315 · Cited by 396
Biological imaging of chemical bonds by stimulated Raman scattering microscopy
Hu, F., Shi, L., & Min, W. (2019) · Nature Methods · doi:10.1038/s41592-019-0538-0 · Cited by 392
Label-free DNA imaging in vivo with stimulated Raman scattering microscopy
Lu, F.-K., Basu, S., Igras, V., Hoang, M. P., Ji, M., Fu, D., Holtom, G. R., Neel, V. A., Freudiger, C. W., Fisher, D. E., et al. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1515121112 · Cited by 302
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Metadata
- Authors
- Fu, D., Lu, F.-K., Zhang, X., Freudiger, C., Pernik, D. R., Holtom, G., & Xie, X. S.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-02-15
- Volume
- 134
- Issue
- 8
- Pages
- 3623-3626
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2132298953
- Cited by
- 295
- References
- 22
- FWCI
- 11.39
- Type
- journal article
- Language
- en
- Open access
- green
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