Characterizing the catecholamine content of single mammalian vesicles by collision-adsorption events at an electrode
Abstract
We present the electrochemical response to single adrenal chromaffin vesicles filled with catecholamine hormones as they are adsorbed and rupture on a 33 μm diameter disk-shaped carbon electrode. The vesicles adsorb onto the electrode surface and sequentially spread out over the electrode surface, trapping their contents against the electrode. These contents are then oxidized, and a current (or amperometric) peak results from each vesicle that bursts. A large number of current transients associated with rupture of single vesicles (86%) are observed under the experimental conditions used, allowing us to quantify the vesicular catecholamine content.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Single-cell amperometry does not distinguish different kinds of catecholamines but can accurately quantify the number of molecules released and the kinetic parameters of each vesicle.
“Single-cell amperometry does not distinguish different kinds of catecholamines, but it can accurately quantify the number of molecules released and the kinetic parameters of each vesicle (Dunevall et al., 2015).”
From Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells (2021) · cites this paper as Dunevall et al., 2015
Cites
Heuser, J. E., Reese, T. S., Dennis, M. J., Jan, Y. N., Jan, L. Y., & Evans, L. (1979) · The Journal of Cell Biology · doi:10.1083/jcb.81.2.275 · Cited by 1,415 · see citing works
Wightman, R. M., Jankowski, J. A., Kennedy, R. T., Kawagoe, K. T., Schroeder, T. J., Leszczyszyn, D. J., Near, J. A., Diliberto, E. J., & Viveros, O. H. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.23.10754 · Cited by 893 · see citing works
The exocytotic event in chromaffin cells revealed by patch amperometry
Albillos, A., Dernick, G., Horstmann, H., Almers, W., de Toledo, G. Á., & Lindau, M. (1997) · Nature · doi:10.1038/39081 · Cited by 568 · see citing works
A simple method for the isolation of adrenal chromaffin granules on a large scale
Smith, A. D., & Winkler, H. J. S. (1967) · Biochemical Journal · doi:10.1042/bj1030480 · Cited by 437 · see citing works
v‐SNAREs control exocytosis of vesicles from priming to fusion
Borisovska, M., Zhao, Y., Tsytsyura, Y., Glyvuk, N., Takamori, S., Matti, U., Rettig, J., Südhof, T. C., & Bruns, D. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600696 · Cited by 244 · see citing works
Kim, B., Boika, A., Kim, J., Dick, J. E., & Bard, A. J. (2014) · Journal of the American Chemical Society · doi:10.1021/ja500713w · Cited by 210 · see citing works
Regulation of Quantal Size by Presynaptic Mechanisms
Sulzer, D., & Pothos, E. N. (2000) · Reviews in the Neurosciences · doi:10.1515/revneuro.2000.11.2-3.159 · Cited by 190 · see citing works
Omiatek, D. M., Dong, Y., Heien, M. L., et al. (2010) · ACS Chemical Neuroscience · doi:10.1021/cn900040e · Cited by 162 · see citing works
Investigation of Single‐Drug‐Encapsulating Liposomes using the Nano‐Impact Method
Cheng, W., & Compton, R. G. (2014) · Angewandte Chemie International Edition · doi:10.1002/anie.201408934 · Cited by 155 · see citing works
Finnegan, J. M., Pihel, K., Cahill, P. S., et al. (1996) · Journal of Neurochemistry · doi:10.1046/j.1471-4159.1996.66051914.x · Cited by 138 · see citing works
Omiatek, D. M., Bressler, A. J., Cans, A.-S. U., Andrews, A. M., Heien, M. L., & Ewing, A. G. (2013) · Scientific Reports · doi:10.1038/srep01447 · Cited by 97 · see citing works
Self-citationPhospholipid mediated plasticity in exocytosis observed in PC12 cells
Uchiyama, Y., Maxson, M. M., Sawada, T., et al. (2007) · Brain Research · doi:10.1016/j.brainres.2007.03.012 · Cited by 89 · see citing works
Amatore, C. A., Arbault, S., Bouret, Y., et al. (2006) · ChemBioChem · doi:10.1002/cbic.200600194 · Cited by 83 · see citing works
Amperometric post spike feet reveal most exocytosis is via extended kiss-and-run fusion
Mellander, L. J., Trouillon, R., Englund, M., et al. (2012) · Scientific Reports · doi:10.1038/srep00907 · Cited by 78 · see citing works
Reconstruction of Aperture Functions during Full Fusion in Vesicular Exocytosis of Neurotransmitters
Amatore, C. A., Igorevich, O. A., & Борисовна, С. И. (2009) · ChemPhysChem · doi:10.1002/cphc.200900647 · Cited by 77 · see citing works
Omiatek, D. M., Santillo, M. F., Heien, M. L., et al. (2009) · Analytical Chemistry · doi:10.1021/ac802466g · Cited by 69 · see citing works
Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide
Reimhult, E., Kasemo, B., & Höök, F. (2009) · International Journal of Molecular Sciences · doi:10.3390/ijms10041683 · Cited by 65 · see citing works
Simulations of Lipid Vesicle Adsorption for Different Lipid Mixtures
Dimitrievski, K., & Kasemo, B. (2008) · Langmuir · doi:10.1021/la703021u · Cited by 56 · see citing works
Recent advances in Electrochemical Detection of Exocytosis
Lemaître, F., Guille‐Collignon, M., & Amatore, C. A. (2014) · Electrochimica Acta · doi:10.1016/j.electacta.2014.02.059 · Cited by 40 · see citing works
Electron Microscopic Evidence for Multiple Types of Secretory Vesicles in Bovine Chromaffin Cells
Koval', L. M., Yavorskaya, E. N., & Lukyanetz, E. A. (2001) · General and Comparative Endocrinology · doi:10.1006/gcen.2000.7592 · Cited by 35 · see citing works
Igorevich, O. A., Lemaître, F., Guille‐Collignon, M., et al. (2013) · Faraday Discussions · doi:10.1039/c3fd00028a · Cited by 34 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Label-Free Detection of Single Living Bacteria via Electrochemical Collision Event
Lee, J. Y., Kim, B.-K., Kang, M., & Park, J. H. (2016) · Scientific Reports · doi:10.1038/srep30022 · Cited by 89 · see citing works
Toward the Detection and Identification of Single Bacteria by Electrochemical Collision Technique
Gao, G., Wang, D., Brocenschi, R., Zhi, J., & Mirkin, M. V. (2018) · Analytical Chemistry · doi:10.1021/acs.analchem.8b03043 · Cited by 74 · see citing works
Single-entity electrochemistry of collision in sensing applications
Sekretareva, A. (2021) · Sensors and Actuators Reports · doi:10.1016/j.snr.2021.100037 · Cited by 41 · see citing works
Detection of Bacterial Rhamnolipid Toxin by Redox Liposome Single Impact Electrochemistry
Luy, J., Ameline, D., Thobie‐Gautier, C., Boujtita, M., & Lebègue, E. (2022) · Angewandte Chemie International Edition · doi:10.1002/anie.202111416 · Cited by 27 · see citing works
Trends in single-impact electrochemistry for bacteria analysis
Smida, H., Langlard, A., Ameline, D., Thobie-Gautier, C., Boujtita, M., & Lebègue, E. (2023) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-023-04568-z · Cited by 22 · see citing works
Intestinal microbiota modulates adrenomedullary response through Nod1 sensing in chromaffin cells
Xiang, C., Chen, P., Zhang, Q., Li, Y., Pan, Y., Xie, W., Sun, J., & Liu, Z. (2021) · iScience · doi:10.1016/j.isci.2021.102849 · Cited by 15 · see citing works
Computer-Assisted Processing of Current Step Signals in Single Blocking Impact Electrochemistry
Langlard, A., Smida, H., Chevalet, R., Thobie-Gautier, C., Boujtita, M., & Lebègue, E. (2024) · ACS Measurement Science Au · doi:10.1021/acsmeasuresciau.4c00046 · Cited by 11 · see citing works
- Authors
- Dunevall, J., Fathali, H., Najafinobar, N., Lovric, J., Wigström, J., Cans, A.-S., & Ewing, A. G.
- Affiliations
- Chalmers University of Technology (SE)
- Department of Chemistry and Chemical Engineering, Chalmers University of Technology, SE-412 96 Gothenburg, Sweden
- Corresponding author
- Ewing, Andrew G.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-03-31
- Volume
- 137
- Issue
- 13
- Pages
- 4344-4346
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2023764829
- Cited by
- 196
- References
- 21
- Citations by year
- 2026: 3 · 2025: 8 · 2024: 9 · 2023: 18 · 2022: 14 · 2021: 21 · 2020: 22 · 2019: 22 · 2018: 19 · 2017: 22 · 2016: 31 · 2015: 7
- FWCI
- 12.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the American Chemical Society (publishedVersion)
- PubMed (publishedVersion)
- Chalmers Publication Library (Chalmers University of Technology) (submittedVersion)
- Chalmers Research (Chalmers University of Technology) (submittedVersion)
- Funders
- National Institutes of Health (R01GM113746)
- European Research Council (267833)
- Knut och Alice Wallenbergs Stiftelse
- Vetenskapsrådet
- Topics
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- catecholamines, chromaffin granules, vesicle rupture, vesicle adsorption, carbon electrodes, amperometric detection, single-vesicle analysis, electrochemical oxidation, adrenal gland
- MeSH terms
- Adrenal Glands, Adsorption, Animals, Carbon, Catecholamines, Cattle, Electrochemistry, Electrodes, Chromaffin Cells
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
