The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Extending the Applicability of Native Chemical Ligation
Abstract
A more general approach to native (amide-forming) chemical ligation of unprotected peptide segments is described that extends the technique beyond the previously reported X-Cys ligation site to now include X-Gly and Gly-X ligation sites. A peptide, [peptide 1 ] α COSR, is reacted with a second peptide, HSCH 2 CH 2 (O)-N α [peptide 2 ], under conditions promoting thioester exchange. The intermediate thioester-linked product rearranges to form a ligation product linked by an N-substituted amide bond. If desired, the -oxyalkyl substitution on the amide bond can be removed by facile treatment with Zn in acidic medium to give a native peptide bond at the ligation site. The techniques described have been employed to ligate small model peptide segments to yield peptides with native or modified backbones, proving the feasibility of this approach.
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Cites
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Self-citationCloning and Characterization of Inducible Nitric Oxide Synthase from Mouse Macrophages
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Self-citationSchnölzer, M., & Kent, S. B. H. (1992) · Science · doi:10.1126/science.1566069 · Cited by 365
Self-citationA Theropod Dinosaur Embryo and the Affinities of the Flaming Cliffs Dinosaur Eggs
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Self-citationBeryllium Binding by Bovine Serum Albumin at Acid pH
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Cited by
Peptide Coupling Reagents, More than a Letter Soup
El-Faham, A., & Albericio, F. (2011) · Chemical Reviews · doi:10.1021/cr100048w · Cited by 1,245
Synthesis of Native Proteins by Chemical Ligation
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Self-citationNative Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations
Agouridas, V., El Mahdi, O., Diemer, V., Cargoët, M., Monbaliu, J.-C. M., & Melnyk, O. (2019) · Chemical Reviews · doi:10.1021/acs.chemrev.8b00712 · Cited by 650
Chemical Synthesis of Proteins
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Rapid and efficient protein synthesis through expansion of the native chemical ligation concept
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Dual Native Chemical Ligation at Lysine
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Metadata
- Authors
- Canne, L. E., Bark, S. J., & Kent, S. B. H.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 1996-01-01
- Volume
- 118
- Issue
- 25
- Pages
- 5891-5896
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1998287079
- Cited by
- 269
- References
- 15
- FWCI
- 2.85
- Type
- journal article
- Language
- en
- Open access
- closed
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