The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
A Nonenzymatic Hairpin DNA Cascade Reaction Provides High Signal Gain of mRNA Imaging inside Live Cells
Open accessAbstract
Enzyme-free signal amplification has enabled sensitive in vitro detection of biomolecules such as proteins and nucleic acids. However, monitoring targets of interest in live cells via enzyme-free amplification is still challenging, especially for analytes with low concentrations. To the best of our knowledge, this paper reports the first attempt to perform mRNA imaging inside live cells, using a nonenzymatic hairpin DNA cascade reaction for high signal gain, termed a hairpin DNA cascade amplifier (HDCA). In conventional nucleic acid probes, such as linear hybridization probes, mRNA target signaling occurs in an equivalent reaction ratio (1:1), whereas, in HDCA, one mRNA target is able to yield multiple signal outputs (1:m), thus achieving the goal of signal amplification for low-expression mRNA targets. Moreover, the recycled mRNA target in the HDCA serves as a catalyst for the assembly of multiple DNA duplexes, generating the fluorescent signal of reduced MnSOD mRNA expression, thus indicating amplified intracellular imaging. This programmable cascade reaction presents a simple and modular amplification mechanism for intracellular biomarkers of interest, providing a significant boost to the search for clues leading to the accurate identification and effective treatment of cancers.
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Cites
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Cited by
Aptamer-integrated DNA nanostructures for biosensing, bioimaging and cancer therapy
Meng, H.-M., Liu, H., Kuai, H., Peng, R., Mo, L., & Zhang, X.-B. (2016) · Chemical Society Reviews · doi:10.1039/c5cs00645g · Cited by 608
mRNA-Initiated, Three-Dimensional DNA Amplifier Able to Function inside Living Cells
He, L., Lu, D., Liang, H., Xie, S., Zhang, X., Liu, Q., Yuan, Q., & Tan, W. (2018) · Journal of the American Chemical Society · doi:10.1021/jacs.7b09789 · Cited by 284
Self-citationPeng, S., Tan, Z., Chen, S., Lei, C., & Nie, Z. (2020) · Chemical Science · doi:10.1039/d0sc03084h · Cited by 284
Nucleic Acid Tests for Clinical Translation
Li, M., Yin, F., Song, L., Mao, X., Li, F., Fan, C., Zuo, X., & Xia, Q. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.1c00241 · Cited by 264
Biostable L-DNAzyme for Sensing of Metal Ions in Biological Systems
Cui, L., Peng, R., Fu, T., Zhang, X., Wu, C., Chen, H., Liang, H., Yang, C. J., & Tan, W. (2016) · Analytical Chemistry · doi:10.1021/acs.analchem.5b04170 · Cited by 81
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Metadata
- Authors
- Wu, C., Cansiz, S., Zhang, L., Teng, I.-T., Qiu, L., Li, J., Liu, Y., Zhou, C., Hu, R., Zhang, T., Cui, C., Cui, L., & Tan, W.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-04-08
- Volume
- 137
- Issue
- 15
- Pages
- 4900-4903
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2067557961
- Cited by
- 332
- References
- 24
- FWCI
- 14.28
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Wu, C., Cansiz, S., Zhang, L., Teng, I.-T., Qiu, L., Li, J., Liu, Y., Zhou, C., Hu, R., Zhang, T., Cui, C., Cui, L., & Tan, W. (2015). A Nonenzymatic Hairpin DNA Cascade Reaction Provides High Signal Gain of mRNA Imaging inside Live Cells. Journal of the American Chemical Society. https://doi.org/10.1021/jacs.5b00542
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