Cited by 52Advanced biosensing and bioanalysis techniquesDNA and Biological ComputingDNA and Nucleic Acid Chemistry

Coupling of DNA Circuit and Templated Reactions for Quadratic Amplification and Release of Functional Molecules

Open access
Kim, Ki Tae, Angerani, Simona, Chang, Dalu, Winssinger, Nicolas·2019·Journal of the American Chemical Society

Abstract

DNA-based circuitry empowers logic gated operations and amplifications but is restricted to nucleic acid output. Templated reactions enable the translation of nucleic acid cues into diverse small-molecule outputs but are more limited in their amplification. Herein, we demonstrate the coupling of a DNA circuit to templated reactions in order to achieve high levels of amplification in the output of small molecules, in response to nucleic acid input. We demonstrate that the coupling of the DNA circuit to templated reactions allows for the detection of the fM concentration of analyte and can respond with the release of a cytotoxic drug.

Integrity signals

Registry

Crossref · 10.1021/jacs.9b05688

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42 of 42 references verified0 citation errors · 0 not checkable

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  • Precedents in templated reactions using DNA:PNA duplexes suggested that a 4-mer PNA should afford efficient primer extension using amide bond formation.

    “Precedents in templated reactions using DNA:PNA duplexes suggested that a 4-mer PNA should afford efficient primer extension using amide bond formation.35”

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Authors
Kim, K. T., Angerani, S., Chang, D., & Winssinger, N.
Affiliations
University of Geneva (CH)
Corresponding author
Winssinger, Nicolas
Venue
Journal of the American Chemical Society
Published
2019-09-20
Volume
141
Issue
41
Pages
16288-16295
ISSN
  • 0002-7863 (ISSN-L)
  • 1520-5126
OpenAlex
W2974428028
Cited by
52
References
42
Citations by year
2026: 1 · 2025: 2 · 2024: 9 · 2023: 9 · 2022: 10 · 2021: 10 · 2020: 11
FWCI
2.56
Type
journal article
Language
en
Open access
Locations
  • Journal of the American Chemical Society (publishedVersion)
  • PubMed (publishedVersion)
  • Archive ouverte UNIGE (University of Geneva) (acceptedVersion, other-oa)
  • Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
Funders
Schweizerischer Nationalfonds zur F?rderung der Wissenschaftlichen Forschung (200020_169141)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Biological Computing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
DNA circuits, drug release, cytotoxic drug delivery, femtomolar detection, nucleic acid sensing
MeSH terms
DNA, Humans, Peptides, Templates, Genetic, Nucleic Acid Amplification Techniques, MicroRNAs

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