Coupling of DNA Circuit and Templated Reactions for Quadratic Amplification and Release of Functional Molecules
Open accessAbstract
DNA-based circuitry empowers logic gated operations and amplifications but is restricted to nucleic acid output. Templated reactions enable the translation of nucleic acid cues into diverse small-molecule outputs but are more limited in their amplification. Herein, we demonstrate the coupling of a DNA circuit to templated reactions in order to achieve high levels of amplification in the output of small molecules, in response to nucleic acid input. We demonstrate that the coupling of the DNA circuit to templated reactions allows for the detection of the fM concentration of analyte and can respond with the release of a cytotoxic drug.
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Precedents in templated reactions using DNA:PNA duplexes suggested that a 4-mer PNA should afford efficient primer extension using amide bond formation.
“Precedents in templated reactions using DNA:PNA duplexes suggested that a 4-mer PNA should afford efficient primer extension using amide bond formation.35”
From Sequencing of <scp>d</scp>/<scp>l</scp>-DNA and XNA by Templated-Synthesis (2025) · cites this paper as [35]
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Self-citationSequencing of <scp>d</scp>/<scp>l</scp>-DNA and XNA by Templated-Synthesis
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- Authors
- Kim, K. T., Angerani, S., Chang, D., & Winssinger, N.
- Affiliations
- University of Geneva (CH)
- Corresponding author
- Winssinger, Nicolas
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2019-09-20
- Volume
- 141
- Issue
- 41
- Pages
- 16288-16295
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2974428028
- Cited by
- 52
- References
- 42
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 9 · 2023: 9 · 2022: 10 · 2021: 10 · 2020: 11
- FWCI
- 2.56
- Type
- journal article
- Language
- en
- Open access
- Licence
- Locations
- Journal of the American Chemical Society (publishedVersion)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (acceptedVersion, other-oa)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- Schweizerischer Nationalfonds zur F?rderung der Wissenschaftlichen Forschung (200020_169141)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Biological Computing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- DNA circuits, drug release, cytotoxic drug delivery, femtomolar detection, nucleic acid sensing
- MeSH terms
- DNA, Humans, Peptides, Templates, Genetic, Nucleic Acid Amplification Techniques, MicroRNAs
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