Effect of transgenes on global gene expression in soybean is within the natural range of variation of conventional cultivars
Abstract
Current safety assessment for novel crops, including transgenic crops, uses a targeted approach, which relies on compositional analysis. The possibility that transgene expression could lead to unintended effects remains a debated issue. This study used transcriptome profiling as a nontargeted approach to evaluate overall molecular changes in transgenic soybean cultivars. Global gene expression was measured in the first trifoliate leaves of two transgenic and three conventional soybean cultivars using the soybean Affymetrix GeneChip. It was found that gene expression differs more between the two conventional cultivars than between the transgenics and their closest conventional cultivar investigated and that the magnitudes of differences measured in gene expression and genotype (determined by SSR analysis) do not necessarily correlate. A MySQL database coupled with a CGI Web interface was developed to store and present the results ( http://soyxpress.agrenv.mcgill.ca/). By integrating the microarray data with gene annotations and other soybean data, a comprehensive view of differences in gene expression is explored between cultivars.
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Independent studies have shown the compositional equivalence of the current generation of GE crops.
“Independent studies have shown the compositional equivalence of the current generation of GE crops (Cheng et al., 2008; Garcia-Villalba et al., 2008; Herman and Price, 2013; Hollingworth et al., 2003)”
From Prevalence and impacts of genetically engineered feedstuffs on livestock populations1 (2014) · cites this paper as Cheng et al., 2008
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- Authors
- Cheng, K. C., Beaulieu, J., Iquira, E., Belzile, F. J., Fortin, M. G., & Strömvik, M. V.
- Affiliations
- Université Laval (CA)
- Corresponding author
- Strömvik, M. V.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-04-23
- Volume
- 56
- Issue
- 9
- Pages
- 3057-3067
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2019524306
- Cited by
- 76
- References
- 30
- Citations by year
- 2026: 2 · 2025: 2 · 2023: 1 · 2022: 4 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 3 · 2015: 6 · 2014: 11 · 2013: 6 · 2012: 5
- FWCI
- 9.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Soybean genetics and cultivation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- transgenic soybean, gene expression, gene expression profiling, unintended consequences, safety assessment, cultivar differences, genotype-phenotype correlation, microarray data integration, genome annotation, microsatellite markers
- MeSH terms
- Genotype, Glycine max, Species Specificity, Gene Expression, Plant Leaves, Transgenes, Gene Expression Profiling, Plants, Genetically Modified
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