Time course production of urolithins from ellagic acid by human gut microbiota
Abstract
Ellagic acid (EA) is converted to urolithins by gut microbiota. Urolithins have beneficial biological effects in humans, but differences in urolithin production capacity among individuals have been shown. Therefore, the identification of the urolithin production pathways and the microorganisms implicated is of high interest. EA was incubated with gut microbiota from two volunteers able to produce urolithins but with different in vivo urolithin profiles (urolithin A and isourolithin A producers). The metabolic capabilities observed in vivo were retained in vitro. Both individuals showed a much higher abundance of Clostridium leptum group of Firmicutes phylum than Bacteroides / Prevotella . EA was either dissolved in DMSO or suspended in water. DMSO increased EA solubility but decreased urolithin production rate due to a delay in growth of some microbial groups, principally, Clostridium coccoides . This allowed the detection of catabolic intermediates [urolithins M-5, M-6, M-7, C, and 2,3,8,10-tetrahydroxy urolithin (urolithin E)]. Bacteria from C. coccoides group (or genera co-occurring in vivo with this group) seem to be involved in production of different urolithins.
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UroA can only be detected in ∼40%-50% of human subjects upon consumption of EA/ET-containing diets.
“However, UroA can only be detected in ∼ 40%–50% of human subjects upon consumption of EA/ET-containing diets.131,136, 137, 138, 139, 140, 141, 142, 143”
From Regulation of Intestinal Barrier Function by Microbial Metabolites (2021) · cites this paper as [137]
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- Authors
- García-Villalba, R., Beltrán, D., Espín, J. C., Selma, M. V., & Tomás-Barberán, F. A.
- Affiliations
- Research Group on Quality, Safety and Bioactivity of Plant Foods, CEBAS-CSIC, Espinardo, Murcia 30100, Spain
- Centro de Edafología y Biología Aplicada del Segura (ES)
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-09-06
- Volume
- 61
- Issue
- 37
- Pages
- 8797-8806
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2329681600
- Cited by
- 207
- References
- 26
- Citations by year
- 2026: 15 · 2025: 22 · 2024: 21 · 2023: 17 · 2022: 25 · 2021: 15 · 2020: 23 · 2019: 14 · 2018: 7 · 2017: 13 · 2016: 13 · 2015: 11 · 2014: 10
- FWCI
- 10.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (acceptedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Pomegranate: compositions and health benefits
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Keywords
- ellagic acid, urolithins, gut microbiota, Clostridium coccoides, urolithin A, polyphenol metabolism
- MeSH terms
- Adult, Bacteria, Coumarins, Ellagic Acid, Feces, Female, Humans, Male, Gastrointestinal Tract, Microbiota
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