Cited by 188Nanoparticle-Based Drug DeliveryRNA Interference and Gene DeliveryProteins in Food Systems

Direct delivery of functional proteins and enzymes to the cytosol using nanoparticle-stabilized nanocapsules

Open access
Tang, Rui, Kim, Chang Soo, Solfiell, David J., Rana, Subinoy, Mout, Rubul, Velázquez-Delgado, Elih M., Chompoosor, Apiwat, Jeong, Youngdo +5 more·2013·ACS Nano

Abstract

Intracellular protein delivery is an important tool for both therapeutic and fundamental applications. Effective protein delivery faces two major challenges: efficient cellular uptake and avoiding endosomal sequestration. We report here a general strategy for direct delivery of functional proteins to the cytosol using nanoparticle-stabilized capsules (NPSCs). These NPSCs are formed and stabilized through supramolecular interactions between the nanoparticle, the protein cargo, and the fatty acid capsule interior. The NPSCs are ~130 nm in diameter and feature low toxicity and excellent stability in serum. The effectiveness of these NPSCs as therapeutic protein carriers was demonstrated through the delivery of fully functional caspase-3 to HeLa cells with concomitant apoptosis. Analogous delivery of green fluorescent protein (GFP) confirmed cytosolic delivery as well as intracellular targeting of the delivered protein, demonstrating the utility of the system for both therapeutic and imaging applications.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1021/nn402753y

Cites

47 of 47 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Tang, R., Kim, C. S., Solfiell, D. J., Rana, S., Mout, R., Velázquez-Delgado, E. M., Chompoosor, A., Jeong, Y., Yan, B., Zhu, Z.-J., Kim, C., Hardy, J. A., & Rotello, V. M.
Affiliations
University of Massachusetts Amherst (US)
Corresponding author
Rotello, Vincent M.
Venue
ACS Nano
Published
2013-07-08
Volume
7
Issue
8
Pages
6667-6673
ISSN
  • 1936-0851 (ISSN-L)
  • 1936-086X
OpenAlex
W2021929306
Cited by
188
References
47
Citations by year
2026: 2 · 2025: 4 · 2024: 6 · 2023: 11 · 2022: 9 · 2021: 16 · 2020: 18 · 2019: 18 · 2018: 21 · 2017: 27 · 2016: 18 · 2015: 18 · 2014: 19 · 2013: 1
FWCI
7.12
Type
journal article
Language
en
Open access
Locations
  • ACS Nano (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • U.S. Public Health Service (S11110000000104)
  • National Institute of General Medical Sciences (GM-077173, R01GM080532, T32GM008515, GM80532, R01GM077173)
  • National Institute of Biomedical Imaging and Bioengineering (R01EB014277, EB014277)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
Keywords
intracellular protein delivery, cytosolic protein delivery, supramolecular interactions, endosomal escape, caspase-3, apoptosis, green fluorescent protein, subcellular targeting, serum stability
MeSH terms
Cytoplasm, Cytosol, Drug Carriers, Flow Cytometry, HeLa Cells, Humans, Ligands, Proteins, Endosomes, Apoptosis, Green Fluorescent Proteins, Caspase 3, Nanoparticles, Nanocapsules

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review