Direct delivery of functional proteins and enzymes to the cytosol using nanoparticle-stabilized nanocapsules
Open accessAbstract
Intracellular protein delivery is an important tool for both therapeutic and fundamental applications. Effective protein delivery faces two major challenges: efficient cellular uptake and avoiding endosomal sequestration. We report here a general strategy for direct delivery of functional proteins to the cytosol using nanoparticle-stabilized capsules (NPSCs). These NPSCs are formed and stabilized through supramolecular interactions between the nanoparticle, the protein cargo, and the fatty acid capsule interior. The NPSCs are ~130 nm in diameter and feature low toxicity and excellent stability in serum. The effectiveness of these NPSCs as therapeutic protein carriers was demonstrated through the delivery of fully functional caspase-3 to HeLa cells with concomitant apoptosis. Analogous delivery of green fluorescent protein (GFP) confirmed cytosolic delivery as well as intracellular targeting of the delivered protein, demonstrating the utility of the system for both therapeutic and imaging applications.
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Direct transmembrane delivery of particles into cytosol bypassing endo-lysosomes may be alternative way.
“In fact, direct transmembrane delivery of the particles into the cytosol bypassing the endo-lysosomes may be an alternative way [187, 188].”
From Non-viral delivery systems for CRISPR/Cas9-based genome editing: challenges and opportunities (2018) · cites this paper as [188]
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- Authors
- Tang, R., Kim, C. S., Solfiell, D. J., Rana, S., Mout, R., Velázquez-Delgado, E. M., Chompoosor, A., Jeong, Y., Yan, B., Zhu, Z.-J., Kim, C., Hardy, J. A., & Rotello, V. M.
- Affiliations
- University of Massachusetts Amherst (US)
- Corresponding author
- Rotello, Vincent M.
- ORCID
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-07-08
- Volume
- 7
- Issue
- 8
- Pages
- 6667-6673
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- PMC
- PMC3757120
- OpenAlex
- W2021929306
- Cited by
- 188
- References
- 47
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 6 · 2023: 11 · 2022: 9 · 2021: 16 · 2020: 18 · 2019: 18 · 2018: 21 · 2017: 27 · 2016: 18 · 2015: 18 · 2014: 19 · 2013: 1
- FWCI
- 7.12
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- ACS Nano (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Public Health Service (S11110000000104)
- National Institute of General Medical Sciences (GM-077173, R01GM080532, T32GM008515, GM80532, R01GM077173)
- National Institute of Biomedical Imaging and Bioengineering (R01EB014277, EB014277)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Keywords
- intracellular protein delivery, cytosolic protein delivery, supramolecular interactions, endosomal escape, caspase-3, apoptosis, green fluorescent protein, subcellular targeting, serum stability
- MeSH terms
- Cytoplasm, Cytosol, Drug Carriers, Flow Cytometry, HeLa Cells, Humans, Ligands, Proteins, Endosomes, Apoptosis, Green Fluorescent Proteins, Caspase 3, Nanoparticles, Nanocapsules
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