Cited by 544Nanoparticle-Based Drug DeliveryRNA Interference and Gene DeliveryDendrimers and Hyperbranched Polymers

Tailoring nanocarriers for intracellular protein delivery

Gu, Zhen, Biswas, Anuradha, Zhao, Muxun, Tang, Yi·2011·Chemical Society Reviews

Abstract

Proteins play a crucial role in life, taking part in all vital processes in the body. In the past decade, there was increasing interest in delivering active forms of proteins to specific cells and organs. Intracellular protein delivery holds enormous promise for biological and medical applications, including cancer therapy, vaccination, regenerative medicine, treatment for loss-of-function genetic diseases and imaging. This tutorial review surveys recent developments in intracellular protein delivery using various nanocarriers. Methods such as lipid-mediated colloidal systems, polymeric nanocarriers, inorganic systems and protein-mediated carriers are reviewed. Advantages and limitations of current strategies, as well as future opportunities and challenges are also discussed.

Integrity signals

Registry

Crossref · 10.1039/c0cs00227e

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90 of 90 references verified0 citation errors · 0 not checkable

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  • Delivery of proteins such as catalase raises concerns of instability in vivo due to physiological proteases, unpredictable protein immunogenicity, and poor in vivo half-life.

    “Delivery of proteins such as catalase raises a few concerns such as its instability in vivo due to the presence of numerous physiological proteases, unpredictable protein immunogenicity, and poor in vivo half-life.27,28”

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Authors
Gu, Z., Biswas, A., Zhao, M., & Tang, Y.
Affiliations
California NanoSystems Institute (US)
Corresponding author
  • Gu, Zhen
  • Tang, Yi
Venue
Chemical Society Reviews
Published
2011
Volume
40
Issue
7
Pages
3638
ISSN
  • 0306-0012 (ISSN-L)
  • 1460-4744
OpenAlex
W2135198179
Cited by
543
References
90
Citations by year
2026: 7 · 2025: 19 · 2024: 28 · 2023: 38 · 2022: 46 · 2021: 47 · 2020: 59 · 2019: 51 · 2018: 56 · 2017: 42 · 2016: 43 · 2015: 32 · 2014: 34 · 2013: 21 · 2012: 13
FWCI
1.44
Type
journal article
Language
en
Open access
closed
Locations
  • Chemical Society Reviews (publishedVersion)
  • PubMed (publishedVersion)
Funders
David and Lucile Packard Foundation
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
Keywords
intracellular protein delivery, nanocarriers, polymeric nanocarriers, inorganic nanocarriers, targeted protein delivery, cancer therapy, vaccination, regenerative medicine, protein imaging
MeSH terms
Animals, Cell Line, Drug Carriers, Humans, Proteins, Protein Transport, Intracellular Space, Nanostructures

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