Tailoring nanocarriers for intracellular protein delivery
Abstract
Proteins play a crucial role in life, taking part in all vital processes in the body. In the past decade, there was increasing interest in delivering active forms of proteins to specific cells and organs. Intracellular protein delivery holds enormous promise for biological and medical applications, including cancer therapy, vaccination, regenerative medicine, treatment for loss-of-function genetic diseases and imaging. This tutorial review surveys recent developments in intracellular protein delivery using various nanocarriers. Methods such as lipid-mediated colloidal systems, polymeric nanocarriers, inorganic systems and protein-mediated carriers are reviewed. Advantages and limitations of current strategies, as well as future opportunities and challenges are also discussed.
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Delivery of proteins such as catalase raises concerns of instability in vivo due to physiological proteases, unpredictable protein immunogenicity, and poor in vivo half-life.
“Delivery of proteins such as catalase raises a few concerns such as its instability in vivo due to the presence of numerous physiological proteases, unpredictable protein immunogenicity, and poor in vivo half-life.27,28”
From Catalase-Integrated Hyaluronic Acid as Nanocarriers for Enhanced Photodynamic Therapy in Solid Tumor (2019) · cites this paper as [28]
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- Authors
- Gu, Z., Biswas, A., Zhao, M., & Tang, Y.
- Affiliations
- California NanoSystems Institute (US)
- Corresponding author
- Gu, Zhen
- Tang, Yi
- Venue
- Chemical Society Reviews
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2011
- Volume
- 40
- Issue
- 7
- Pages
- 3638
- ISSN
- 0306-0012 (ISSN-L)
- 1460-4744
- OpenAlex
- W2135198179
- Cited by
- 543
- References
- 90
- Citations by year
- 2026: 7 · 2025: 19 · 2024: 28 · 2023: 38 · 2022: 46 · 2021: 47 · 2020: 59 · 2019: 51 · 2018: 56 · 2017: 42 · 2016: 43 · 2015: 32 · 2014: 34 · 2013: 21 · 2012: 13
- FWCI
- 1.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemical Society Reviews (publishedVersion)
- PubMed (publishedVersion)
- Funders
- David and Lucile Packard Foundation
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
- Keywords
- intracellular protein delivery, nanocarriers, polymeric nanocarriers, inorganic nanocarriers, targeted protein delivery, cancer therapy, vaccination, regenerative medicine, protein imaging
- MeSH terms
- Animals, Cell Line, Drug Carriers, Humans, Proteins, Protein Transport, Intracellular Space, Nanostructures
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