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Cited by 407Nanoparticle-Based Drug DeliveryPolymer Surface Interaction StudiesDendrimers and Hyperbranched Polymers

Polydopamine-Based Simple and Versatile Surface Modification of Polymeric Nano Drug Carriers

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Park, Joonyoung, Brust, Tarsis F., Lee, Hong Jae, Lee, Sang Cheon, Watts, Val J., Yeo, Yoon·2014·ACS Nano
DOI 10.1021/nn405809cPMC PMC4107448
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Abstract

The surface of a polymeric nanoparticle (NP) is often functionalized with cell-interactive ligands and/or additional polymeric layers to control NP interaction with cells and proteins. However, such modification is not always straightforward when the surface is not chemically reactive. For this reason, most NP functionalization processes employ reactive linkers or coupling agents or involve prefunctionalization of the polymer, which are complicated and inefficient. Moreover, prefunctionalized polymers can lose the ability to encapsulate and retain a drug if the added ligands change the chemical properties of the polymer. To overcome this challenge, we use dopamine polymerization as a way of functionalizing NP surfaces. This method includes brief incubation of the preformed NPs in a weak alkaline solution of dopamine, followed by secondary incubation with desired ligands. Using this method, we have functionalized poly(lactic-co-glycolic acid) (PLGA) NPs with three representative surface modifiers: a small molecule (folate), a peptide (Arg-Gly-Asp), and a polymer [poly(carboxybetaine methacrylate)]. We confirmed that the modified NPs showed the expected cellular interactions with no cytotoxicity or residual bioactivity of dopamine. The dopamine polymerization method is a simple and versatile surface modification method, applicable to a variety of NP drug carriers irrespective of their chemical reactivity and the types of ligands.

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  • Both PDA and its in vivo degradation products display negligible cytotoxicity and immunogenicity.

    “Extensive in vitro cellular assays and in vivo animal studies have confirmed that both PDA and its in vivo degradation products display negligible cytotoxicity and immunogenicity.”

    From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [9]

    The Results and Discussion section, under “Toxicity and biological activity of pD coating” (page not provided), reports no significant cytotoxicity from pD-coated particles or pD aggregates in the tested cells, and cites in vivo findings of low toxicity. It does not establish negligible cytotoxicity or immunogenicity of pD’s in vivo degradation products; it explicitly says pD biodegradation and long-term toxicology remain to be systematically investigated. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

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Cited by

Sorted by citations

Authors
Park, J., Brust, T. F., Lee, H. J., Lee, S. C., Watts, V. J., & Yeo, Y.
Affiliations
  • Purdue University West Lafayette (US)
  • Kyung Hee University (KR)
Corresponding author
Yeo, Yoon
ORCID
Venue
ACS Nano
Publisher
American Chemical Society (ACS)
Published
2014-03-19
Volume
8
Issue
4
Pages
3347-3356
ISSN
  • 1936-0851 (ISSN-L)
  • 1936-086X
DOI
10.1021/nn405809c
PMC
PMC4107448
OpenAlex
W2335405043
Cited by
407
References
4
Citations by year
2026: 13 · 2025: 15 · 2024: 24 · 2023: 34 · 2022: 33 · 2021: 33 · 2020: 37 · 2019: 56 · 2018: 59 · 2017: 42 · 2016: 34 · 2015: 23 · 2014: 4
FWCI
13.02
Type
journal article
Language
en
Open access
Locations
  • ACS Nano (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
National Science Foundation (DMR-1056997)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
Keywords
polydopamine, surface modification, polymeric nanoparticles, drug carriers, PLGA nanoparticles, dopamine polymerization, folic acid, RGD peptide, poly(carboxybetaine methacrylate), ligand conjugation, nanoparticle-cell interactions, nanoparticle-protein interactions
MeSH terms
Polylactic Acid-Polyglycolic Acid Copolymer, Betaine, Biological Transport, Drug Carriers, Folic Acid, Humans, Indoles, KB Cells, Oligopeptides, Particle Size, Polyglycolic Acid, Polymers, Polymethacrylic Acids, Safety, Surface Properties, Lactic Acid, Nanoparticles

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