Small molecule delivery to solid tumors with chitosan-coated PLGA particles: A lesson learned from comparative imaging
Open accessAbstract
For polymeric nanoparticles (NPs) to deliver more drugs to tumors than free drug solution, it is critical that the NPs establish interactions with tumor cells and avoid removal from the tumors. Since traditional polyethylene glycol (PEG) surface layer interferes with the cell-NP interaction in tumors, we used a water-soluble and blood-compatible chitosan derivative called zwitterionic chitosan (ZWC) as an alternative surface coating for poly(lactic-co-glycolic acid) (PLGA) NPs. The ZWC-coated PLGA NPs showed pH-dependent surface charge profiles and differential cellular interactions according to the pH of the medium. The in vivo delivery of ZWC-coated NPs was evaluated in mice bearing LS174T-xenografts using magnetic resonance (MR) imaging and fluorescence whole body imaging, which respectively tracked iron oxide particles and indocyanine green (ICG) encapsulated in the NPs as tracers. MR imaging showed that ZWC-coated NPs were more persistent in tumors than PEG-coated NPs, in agreement with the in vitro results. However, the fluorescence imaging indicated that the increased NP retention in tumors by the ZWC coating did not significantly affect the ICG distribution in tumors due to the rapid release of the dye. This study shows that stable drug retention in NPs during circulation is a critical prerequisite to successful translation of the potential benefits of surface-engineered NPs.
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Utilizing a duple of fluorescent and MRI agents, high sensitivity of fluorescent was accompanied by MRI's aptitude for deep-tissue imaging and high spatial and temporal resolution.
“Utilizing a duple of fluorescent and MRI agents, the high sensitivity of the fluorescent was accompanied by the MRI’s aptitude for deep-tissue imaging and high spatial and temporal resolution [142,143].”
From Approaches to Improve EPR-Based Drug Delivery for Cancer Therapy and Diagnosis (2023) · cites this paper as [142]
Methods §§2.11–2.12 and Results §3.4 (page numbers unavailable) show that the study used MRI and near-infrared fluorescence agents to image tumor-bearing mice. The copy does not characterize fluorescence as highly sensitive or MRI as providing deep-tissue imaging and high spatial and temporal resolution, so those attributed advantages are unsupported. (LLM, conf=0.82)
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Approaches to Improve EPR-Based Drug Delivery for Cancer Therapy and Diagnosis
Subhan, M. A., Parveen, F., Filipczak, N., Yalamarty, S. S. K., & Torchilin, V. P. (2023) · Journal of Personalized Medicine · doi:10.3390/jpm13030389 · Cited by 98 · see citing works
“Utilizing a duple of fluorescent and MRI agents, the high sensitivity of the fluorescent was accompanied by the MRI’s aptitude for deep-tissue imaging and high spatial and temporal resolution [142,143].”
Backgroundde Maar, J. S., Sofias, A. M., Porta Siegel, T., Vreeken, R. J., Moonen, C., Bos, C., & Deckers, R. (2020) · Theranostics · doi:10.7150/thno.38625 · Cited by 71 · see citing works
Optimized indocyanine green nanopreparations for biomedical applications
Xia, W.-L., Ran, X.-Y., Xie, K.-P., Zhao, Y., Chen, J., Zhou, Q., Yu, X.-Q., & Li, K. (2025) · Coordination Chemistry Reviews · doi:10.1016/j.ccr.2024.216422 · Cited by 33 · see citing works
- Authors
- Park, J., Pei, Y., Hyun, H., Castanares, M. A., Collins, D. S., & Yeo, Y.
- Affiliations
- Eli Lilly (United States) (US)
- Purdue University West Lafayette (US)
- Corresponding author
- Yeo, Yoon
- Venue
- Journal of Controlled Release
- Publisher
- Elsevier BV
- Published
- 2017-12
- Volume
- 268
- Pages
- 407-415
- ISSN
- 0168-3659 (ISSN-L)
- PMC
- PMC5722701
- OpenAlex
- W2766837348
- Cited by
- 29
- References
- 37
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 7 · 2020: 3 · 2019: 1 · 2018: 8
- FWCI
- 1.18
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Controlled Release (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Lilly Innovation Fellowship Award (NIH R01 EB017791, NSF DMR-1410987)
- Purdue University (NIH P30 CA023168)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- PLGA nanoparticles, nanoparticle coating, pH-dependent surface charge, indocyanine green, nanoparticle circulation, drug release
- MeSH terms
- Polylactic Acid-Polyglycolic Acid Copolymer, Animals, Drug Carriers, Female, Humans, Magnetic Resonance Imaging, Mice, Inbred BALB C, Mice, Nude, Neoplasms, Polyglycolic Acid, Lactic Acid, Cell Line, Tumor, Chitosan, Mice, Whole Body Imaging, Nanoparticles, Optical Imaging
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