The DOI matches its Crossref record; 64 of the 64 references it cites were found in a registry or an index.
The Platelet Microparticle Proteome
Abstract
Platelet-derived microparticles are the most abundant type of microparticle in human blood and contribute to many biologically significant processes. Here, we report the first proteomic analysis of microparticles generated from activated platelets. Using 1D SDS-PAGE and liquid chromatography coupled to a linear ion trap mass spectrometer, the identification of 578 proteins was accomplished using a minimum of 5 MS/MS detections of at least two different peptides for each protein. These microparticles displayed many proteins intrinsic to and well-characterized on platelets. For example, microparticles in these experiments were found to contain membrane surface proteins including GPIIIa, GPIIb, and P-selectin, as well other platelet proteins such as the chemokines CXCL4, CXCL7, and CCL5. In addition, approximately 380 of the proteins identified were not found in two previous studies of the platelet proteome. Since several of the proteins detected here have been previously implicated in microparticle formation and/or pathological function, it is hoped that this study will help fuel future work concerning the possible role of microparticles in various disease states.
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Cites
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Shedding microvesicles: artefacts no more
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Microparticles: Protagonists of a Novel Communication Network for Intercellular Information Exchange
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Proteomic analysis of platelet α‐granules using mass spectrometry
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Platelets: The missing link between the blood and brain?
Leiter, O., & Walker, T. L. (2019) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2019.101695 · Cited by 103
Metadata
- Authors
- Garcia, B. A., Smalley, D. M., Cho, Shabanowitz, J., Ley, K., & Hunt, D. F.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-07-21
- Volume
- 4
- Issue
- 5
- Pages
- 1516-1521
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W1966830640
- Cited by
- 297
- References
- 64
- FWCI
- 5.30
- Type
- journal article
- Language
- en
- Open access
- closed
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