The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Within-Day Reproducibility of an HPLC−MS-Based Method for Metabonomic Analysis: Application to Human Urine
Abstract
Self-evidently, research in areas supporting "systems biology" such as genomics, proteomics, and metabonomics are critically dependent on the generation of sound analytical data. Metabolic phenotyping using LC-MS-based methods is currently at a relatively early stage of development, and approaches to ensure data quality are still developing. As part of studies on the application of LC-MS in metabonomics, the within-day reproducibility of LC-MS, with both positive and negative electrospray ionization (ESI), has been investigated using a standard "quality control" (QC) sample. The results showed that the first few injections on the system were not representative, and should be discarded, and that reproducibility was critically dependent on signal intensity. On the basis of these findings, an analytical protocol for the metabonomic analysis of human urine has been developed with proposed acceptance criteria based on a step-by-step assessment of the data. Short-term sample stability for human urine was also assessed. Samples were stable for at least 20 h at 4 degrees C in the autosampler while queuing for analysis. Samples stored at either -20 or -80 degrees C for up to 1 month were indistinguishable on subsequent LC-MS analysis. Overall, by careful monitoring of the QC data, it is possible to demonstrate that the "within-day" reproducibility of LC-MS is sufficient to ensure data quality in global metabolic profiling applications.
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Cites
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Metabonomics: a platform for studying drug toxicity and gene function
Nicholson, J. K., Connelly, J., Lindon, J. C., & Holmes, E. (2002) · Nature Reviews Drug Discovery · doi:10.1038/nrd728 · Cited by 1,914
High resolution proton magnetic resonance spectroscopy of biological fluids
Nicholson, J. K., & Wilson, I. D. (1989) · Progress in Nuclear Magnetic Resonance Spectroscopy · doi:10.1016/0079-6565(89)80008-1 · Cited by 625
Self-citationSangster, T., Major, H., Plumb, R., Wilson, A. J., & Wilson, I. D. (2006) · The Analyst · doi:10.1039/b604498k · Cited by 564
Self-citationDiscovery of Urinary Biomarkers
Pisitkun, T., Johnstone, R., & Knepper, M. A. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.r600004-mcp200 · Cited by 405
Plumb, R. S., Stumpf, C. L., Gorenstein, M. V., Castro‐Perez, J. M., Dear, G. J., Anthony, M., Sweatman, B. C., Connor, S. C., & Haselden, J. N. (2002) · Rapid Communications in Mass Spectrometry · doi:10.1002/rcm.813 · Cited by 216
Lauridsen, M., Hansen, S. H., Jaroszewski, J. W., & Cornett, C. (2007) · Analytical Chemistry · doi:10.1021/ac061354x · Cited by 179
Welthagen, W., Shellie, R. A., Spranger, J., Ristow, M., Zimmermann, R., & Fiehn, O. (2005) · Metabolomics · doi:10.1007/s11306-005-1108-2 · Cited by 165
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Toxicological applications of magnetic resonance
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Cited by
Global metabolic profiling of animal and human tissues via UPLC-MS
Want, E. J., Masson, P., Michopoulos, F., Wilson, I. D., Theodoridis, G., Plumb, R. S., Shockcor, J., Loftus, N., Holmes, E., & Nicholson, J. K. (2013) · Nature Protocols · doi:10.1038/nprot.2012.135 · Cited by 1,611
Self-citationScalbert, A., Brennan, L., Fiehn, O., Hankemeier, T., Kristal, B. S., van Ommen, B., Pujos-Guillot, E., Verheij, E., Wishart, D., & Wopereis, S. (2009) · Metabolomics · doi:10.1007/s11306-009-0168-0 · Cited by 565
LC-MS-based methodology for global metabolite profiling in metabonomics/metabolomics
Theodoridis, G., Gika, H. G., & Wilson, I. D. (2008) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2008.01.008 · Cited by 371
Self-citationMethod validation strategies involved in non-targeted metabolomics
Naz, S., Vallejo, M., García, A., & Barbas, C. (2014) · Journal of Chromatography A · doi:10.1016/j.chroma.2014.04.071 · Cited by 332
van der Kloet, F. M., Bobeldijk, I., Verheij, E. R., & Jellema, R. H. (2009) · Journal of Proteome Research · doi:10.1021/pr900499r · Cited by 317
Metadata
- Authors
- Gika, H. G., Theodoridis, G. A., Wingate, J. E., & Wilson, I. D.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-07-11
- Volume
- 6
- Issue
- 8
- Pages
- 3291-3303
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W1994397833
- Cited by
- 502
- References
- 13
- FWCI
- 9.22
- Type
- journal article
- Language
- en
- Open access
- closed
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- Gika, H. G., Theodoridis, G. A., Wingate, J. E., & Wilson, I. D. (2007). Within-Day Reproducibility of an HPLC−MS-Based Method for Metabonomic Analysis: Application to Human Urine. Journal of Proteome Research. https://doi.org/10.1021/pr070183p
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