The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
A Comparison of Labeling and Label-Free Mass Spectrometry-Based Proteomics Approaches
Abstract
The proteome of the recently discovered bacterium Methylocella silvestris has been characterized using three profiling and comparative proteomics approaches. The organism has been grown on two different substrates enabling variations in protein expression to be identified. The results obtained using the experimental approaches have been compared with respect to number of proteins identified, confidence in identification, sequence coverage and agreement of regulated proteins. The sample preparation, instrumental time and sample loading requirements of the differing experiments are compared and discussed. A preliminary screen of the protein regulation results for biological significance has also been performed.
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Cites
Ong, S.-E., Blagoev, B., Kratchmarova, I., Kristensen, D. B., Steen, H., Pandey, A., & Mann, M. (2002) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m200025-mcp200 · Cited by 5,574
Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
Gygi, S. P., Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H., & Aebersold, R. (1999) · Nature Biotechnology · doi:10.1038/13690 · Cited by 4,683 · cited 1×
“peptide standards [11], tandem mass tags (TMT) [12], isotope1coded affinity tags (ICAT) [ 13 ], and more”
BackgroundRoss, P. L., Huang, Y. N., Marchese, J. N., Williamson, B., Parker, K., Hattan, S., Khainovski, N., Pillai, S., Dey, S., Daniels, S., et al. (2004) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m400129-mcp200 · Cited by 4,401
Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS
Gerber, S. A., Rush, J., Stemman, O., Kirschner, M. W., & Gygi, S. P. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0832254100 · Cited by 1,733
Absolute Quantification of Proteins by LCMSE
Silva, J. C., Gorenstein, M. V., Li, G.-Z., Vissers, J. P. C., & Geromanos, S. J. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500230-mcp200 · Cited by 1,529
Cited by
Mass Spectrometry-Based Label-Free Quantitative Proteomics
Zhu, W., Smith, J. W., & Huang, C.-M. (2010) · Journal of Biomedicine and Biotechnology · doi:10.1155/2010/840518 · Cited by 629
Role of spectral counting in quantitative proteomics
Lundgren, D. H., Hwang, S.-I., Wu, L., & Han, D. K. (2010) · Expert Review of Proteomics · doi:10.1586/epr.09.69 · Cited by 428
Current trends in quantitative proteomics
Elliott, M. H., Smith, D. S., Parker, C. E., & Borchers, C. (2009) · Journal of Mass Spectrometry · doi:10.1002/jms.1692 · Cited by 271
Quantitative profiling of the protein coronas that form around nanoparticles
Docter, D., Distler, U., Storck, W., Kuharev, J., Wünsch, D., Hahlbrock, A., Knauer, S. K., Tenzer, S., & Stauber, R. H. (2014) · Nature Protocols · doi:10.1038/nprot.2014.139 · Cited by 253
Guarnieri, M. T., Nag, A., Smolinski, S. L., Darzins, A., Seibert, M., & Pienkos, P. T. (2011) · PLoS ONE · doi:10.1371/journal.pone.0025851 · Cited by 234
Metadata
- Authors
- Patel, V. J., Thalassinos, K., Slade, S. E., Connolly, J. B., Crombie, A., Murrell, J. C., & Scrivens, J. H.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-06-08
- Volume
- 8
- Issue
- 7
- Pages
- 3752-3759
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2128969781
- Cited by
- 258
- References
- 5
- FWCI
- 8.11
- Type
- journal article
- Language
- en
- Open access
- closed
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