The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Current trends in quantitative proteomics
Open accessAbstract
It was inevitable that as soon as mass spectrometrists were able to tell biologists which proteins were in their samples, the next question would be how much of these proteins were present. This has turned out to be a much more challenging question. In this review, we describe the multiple ways that mass spectrometry has attempted to address this issue, both for relative quantitation and for absolute quantitation of proteins. There is no single method that will work for every problem or for every sample. What we present here is a variety of techniques, with guidelines that we hope will assist the researcher in selecting the most appropriate technique for the particular biological problem that needs to be addressed. We need to emphasize that this is a very active area of proteomics research—new quantitative methods are continuously being introduced and some ‘pitfalls’ of older methods are just being discovered. However, even though there is no perfect technique—and a better technique may be developed tomorrow—valuable information on biomarkers and pathways can be obtained using these currently available methods Copyright © 2009 John Wiley & Sons, Ltd.
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“to grow in the dialyzed serum required for SILAC due to the loss of essential growth factors.[1] The isotope distributions resulting from the combination of 13C and 15N can be difficult to interpret.”
BackgroundMethodInfluentialAbsolute Quantification of Proteins by LCMSE
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Cited by
Bioanalytical Chemistry of Cytokines–a Review
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CE‐MS‐based proteomics in biomarker discovery and clinical application
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A practical data processing workflow for multi-OMICS projects
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“) strategies (for review see [56–58]).”
Capillary electrophoresis with Orbitrap-Velos mass spectrometry detection
Wojcik, R., Li, Y., MacCoss, M. J., & Dovichi, N. J. (2012) · Talanta · doi:10.1016/j.talanta.2011.10.048 · Cited by 43
Metadata
- Authors
- Elliott, M. H., Smith, D. S., Parker, C. E., & Borchers, C.
- Venue
- Journal of Mass Spectrometry
- Publisher
- Wiley
- Published
- 2009-12
- Volume
- 44
- Issue
- 12
- Pages
- 1637-1660
- ISSN
- 1076-5174 (ISSN-L)
- 1096-9888
- OpenAlex
- W2022794640
- Cited by
- 271
- References
- 14
- FWCI
- 15.28
- Type
- journal article
- Language
- en
- Open access
- bronze
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