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The use of UCOE vectors in combination with a preadapted serum free, suspension cell line allows for rapid production of large quantities of protein
Open accessAbstract
UCOE vectors contain non-tissue specific chromatin-opening-elements that permit rapid expression of a protein in anintegration independent manner. Efficient expression can bederived from a single copy of an integrated gene site resulting ina higher percentage of cells expressing the marker gene in theselected pool in comparison to standard non-UCOE containingvectors. This, in combination with the utilization of a serum-free, suspension adapted parent cell line allows for rapidproduction of large quantities of protein in a short period oftime. Utilizing this system more than 300 mg of a recombinantantibody has been produced in less than 1 month from transfectionpools in shake flask. Selected subclones have been scaled intosmall bioreactors in less than 2 months, producing significantquantities of monoclonal antibody using a protocol generic for theparent cell line. The increased efficiency obtained with the UCOEvector reduces the number of transfectants which need to bescreened in order to obtain high productivity subclones.Transfection of a standard host cell line, preadapted to grow in alarge-scale setting, allows for rapid cell line developmentdecreasing the transition time from research into development andmanufacturing. Alternatively, the traditional approach of using aparent cell line which requires serum-free and suspensionadaptation after transfection further increases the need forscreening a large number of subclones, because many of thesubclones will not be able to grow under conditions that allowlarge-scale protein production. The use of a preadapted cell linecan reduce the time required to develop a cell line from months toweeks.
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“UCOEs were also found to be helpful for increasing the productivity of recombinant protein cell lines (Benton et al., 2002;She et al., 2009).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Benton et al., 2002
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Research works printed but not linked: 9
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Cited by
Kim, J. Y., Kim, Y.-G., & Lee, G. M. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3758-5 · Cited by 801
Advances in Mammalian cell line development technologies for recombinant protein production
Lai, T., Yang, Y., & Ng, S. (2013) · Pharmaceuticals · doi:10.3390/ph6050579 · Cited by 322
Therapeutic glycoprotein production in mammalian cells
Lalonde, M.-E., & Durocher, Y. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.04.028 · Cited by 296
Osterlehner, A., Simmeth, S., & Göpfert, U. (2011) · Biotechnology and Bioengineering · doi:10.1002/bit.23216 · Cited by 131
Fan, L., Frye, C. C., & Racher, A. J. (2013) · Pharmaceutical Bioprocessing · doi:10.4155/pbp.13.56 · Cited by 35
Metadata
- Authors
- Benton, T., Chen, T., McEntee, M., Fox, B., King, D., Crombie, R., Thomas, T. C., & Bebbington, C.
- Affiliations
- Corixa Corporation (US)
- Keele University (GB)
- Venue
- Cytotechnology
- Published
- 2002-01
- Volume
- 38
- Issue
- 1-3
- Pages
- 43-46
- ISSN
- 0920-9069 (ISSN-L)
- 1573-0778
- PMC
- PMC3449923
- OpenAlex
- W2113446079
- Cited by
- 98
- References
- 0
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 7 · 2016: 2 · 2015: 11 · 2014: 5 · 2013: 10 · 2012: 7
- FWCI
- 0.35
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springernature.com/gp/researchers/text-and-data-mining (tdm)
- www.springernature.com/gp/researchers/text-and-data-mining (vor)
- Locations
- Cytotechnology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- cell line development, shake flask culture, bioreactor scale-up, large-scale protein production
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- CoChat. (2026). Citation check: The use of UCOE vectors in combination with a preadapted serum free, suspension cell line allows for rapid production of large quantities of protein. https://app.cochat.ai/papers/10.1023/a:1021141712344
- The paper
- Benton, T., Chen, T., McEntee, M., Fox, B., King, D., Crombie, R., Thomas, T. C., & Bebbington, C. (2002). The use of UCOE vectors in combination with a preadapted serum free, suspension cell line allows for rapid production of large quantities of protein. Cytotechnology. https://doi.org/10.1023/a%3A1021141712344
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
