Cited by 35Viral Infectious Diseases and Gene Expression in InsectsMonoclonal and Polyclonal Antibodies ResearchProtein purification and stability

The use of glutamine synthetase as a selection marker: recent advances in Chinese hamster ovary cell line generation processes

Fan, Lianchun, Frye, Christopher C, Racher, Andrew J·2013·Pharmaceutical Bioprocessing

Abstract

Central to the needs of the biopharmaceutical industry to support the development of innovative biologics and biosimilars are more effective and efficient manufacturing processes which require highly productive cell lines with desired quality attributes. New designs of molecules such as antibody humanization have greatly reduced immunogenicity concerns, and advances in cell culture technology including media optimization and process control have driven monoclonal antibody productivities in excess of 10 g/l with peak cell densities in bioreactors climbing to over 35 million per ml. However, over this same timeframe, the fundamental processes utilized for cell line generation have not changed significantly, especially in the selection step for top-producing clonal cell lines. Cell line generation continues to be time consuming and labor intensive and has become the timeline limiting step for the majority of the industry. In order to meet the ‘Fast-to-Proof-of-Principle’ strategy, multiple efforts including ...

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Crossref · 10.4155/pbp.13.56

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123 of 123 references verified0 citation errors · 0 not checkable

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  • The GS selection system uses complementation of a glutamine auxotrophy by a recombinant GS gene.

    “The first one, originally developed by Celltech (now Lonza Biologics), uses complementation of a glutamine auxotrophy by a recombinant GS gene (Fan et al., 2013).”

    From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Fan et al., 2013

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Authors
Fan, L., Frye, C. C., & Racher, A. J.
Venue
Pharmaceutical Bioprocessing
Published
2013-12
Volume
1
Issue
5
Pages
487-502
ISSN
  • 2048-9145 (ISSN-L)
  • 2048-9153
OpenAlex
W2002725152
Cited by
35
References
124
Citations by year
2026: 2 · 2025: 3 · 2024: 1 · 2023: 4 · 2022: 4 · 2021: 4 · 2020: 5 · 2019: 4 · 2018: 2 · 2017: 3 · 2016: 2 · 2014: 1
FWCI
0.45
Type
journal article
Language
en
Open access
closed
Locations
Pharmaceutical Bioprocessing (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
Keywords
glutamine synthetase, selectable marker, CHO cells, cell line establishment, monoclonal antibody production, clonal cell lines, biopharmaceutical manufacturing, antibody humanization, biosimilars

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