Local production and detection of (specific) IgE in nasal B-cells and plasma cells of allergic rhinitis patients
Open accessAbstract
Allergic diseases are characterized by allergic complaints in the shock organ and specific immunoglobulin (Ig)E in serum. Literature data indicate that the nasal mucosa itself could produce at least a large part of the specific IgE in allergic rhinitis patients. In order to investigate this hypothesis, nasal mucosal biopsies from the inferior turbinate were taken from symptomatic grass pollen allergic rhinitis patients, symptomatic house dust mite allergic rhinitis patients and nonallergic healthy controls, confirmed by radioallergosorbent test and skin-prick test. Immunohistochemical double-staining was performed for B-cells (CD19) with IgE, plasma cells (CD138) with IgE and plasma cells with biotinylated allergens. Significantly more IgE-positive B-cells and IgE-positive plasma cells were found in the nasal mucosa of allergic patients than in that of nonallergic controls. Double staining with biotinylated allergens and plasma cells showed allergen-positive plasma cells in the nasal mucosa of allergic patients and no allergen-positive plasma cells in the nasal mucosa of nonallergic patients. Blocking experiments using polyclonal antibodies directed against IgE showed a significant reduction in the number of allergen-positive cells in contrast to experiments using polyclonal antibodies directed against IgG, IgA or IgM. This study describes new evidence that specific immunoglobulin E is produced locally in the nasal mucosa in patients with seasonal allergic rhinitis and perennial allergic rhinitis, but not in nonallergic controls.
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Newer evidence identifies B-cells and plasma cells capable of producing IgE in nasal tissue of AR patients.
“Although it was believed that IgE-producing B-cells reside in lymphoid follicles of the Waldeyer ring376 and antibodies were then transferred to the mucosa, newer evidence has identified B-cells and plasma cells capable of producing IgE in nasal tissue of AR patients.377”
From International Consensus Statement on Allergy and Rhinology: Allergic Rhinitis (2018) · cites this paper as [377]
Allergic rhinitis patients have specific IgE in the nasal mucosa, with evidence that class switching and antibody production occur locally.
“In addition to positive systemic sIgE, AR patients have been shown to have sIgE in the nasal mucosa with evidence that class switching and antibody production occurs locally.309-312,377,950,951”
From International Consensus Statement on Allergy and Rhinology: Allergic Rhinitis (2018) · cites this paper as [377]
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- Authors
- KleinJan, A., Vinke, J. G., Severijnen, L. W. F. M., & Fokkens, W. J.
- Affiliations
- Erasmus MC (NL)
- Venue
- European Respiratory Journal
- Publisher
- European Respiratory Society (ERS)
- Published
- 2000-03
- Volume
- 15
- Issue
- 3
- Pages
- 491-497
- ISSN
- 0903-1936 (ISSN-L)
- 1399-3003
- OpenAlex
- W2156278784
- Cited by
- 181
- References
- 32
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 2 · 2023: 8 · 2022: 8 · 2021: 5 · 2020: 4 · 2019: 6 · 2018: 7 · 2017: 7 · 2016: 8 · 2015: 8 · 2014: 7 · 2013: 8 · 2012: 8
- FWCI
- 2.47
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- European Respiratory Journal (publishedVersion)
- PubMed (publishedVersion)
- Pure Amsterdam UMC (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (publishedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion, other-oa)
- RePub (Erasmus University Rotterdam) (submittedVersion, other-oa)
- RePub (Erasmus University Rotterdam) (submittedVersion)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Allergic Rhinitis and Sensitization
- Health Sciences › Medicine › Physiology › Asthma and respiratory diseases
- Health Sciences › Medicine › Immunology and Allergy › Food Allergy and Anaphylaxis Research
- Keywords
- allergic rhinitis, nasal mucosa, B cells, plasma cells, grass pollen allergy, house dust mite allergy, specific IgE, inferior turbinate
- MeSH terms
- Adolescent, Adult, B-Lymphocytes, Female, Rhinitis, Allergic, Seasonal, Humans, Immunoglobulin E, Immunohistochemistry, Male, Middle Aged, Nose, Plasma Cells, Rhinitis, Allergic, Perennial
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