Early nucleolar disorganization in Dictyostelium cell death
Open accessAbstract
Cell death occurs in all eukaryotes, but it is still not known whether some core steps of the cell death process are conserved. We investigated this using the protist Dictyostelium. The dissection of events in Dictyostelium vacuolar developmental cell death was facilitated by the sequential requirement for two distinct exogenous signals. An initial exogenous signal (starvation and cAMP) recruited some cells into clumps. Only within these clumps did subsequent cell death events take place. Contrary to our expectations, already this initial signal provoked nucleolar disorganization and irreversible inhibition of rRNA and DNA synthesis, reflecting marked cell dysfunction. The initial signal also primed clumped cells to respond to a second exogenous signal (differentiation-inducing factor-1 or c-di-GMP), which led to vacuolization and synthesis of cellulose encasings. Thus, the latter prominent hallmarks of developmental cell death were induced separately from initial cell dysfunction. We propose that (1) in Dictyostelium vacuolization and cellulose encasings are late, organism-specific, hallmarks, and (2) on the basis of our observations in this protist and of similar previous observations in some cases of mammalian cell death, early inhibition of rRNA synthesis and nucleolar disorganization may be conserved in some eukaryotes to usher in developmental cell death.
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In D. discoideum, potentially lethal ATP depletion rapidly causes nucleolar disorganization and irreversible block in rRNA and DNA synthesis.
“Interestingly, one of the most rapid consequences of potentially lethal ATP depletion in D. discoideum is an abrupt nucleolar disorganization coupled to an irreversible block in ribosomal RNA and DNA synthesis [1050].”
From Molecular mechanisms of cell death: recommendations of the nomenclature committee on cell death 2018. (2018) · cites this paper as [1050]
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Self-citation
- Authors
- Luciani, M. F., Song, Y., Sahrane, A., Kosta, A., & Golstein, P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Golstein, P
- Venue
- Cell Death & Disease
- Published
- 2017-01-05
- Volume
- 8
- Issue
- 1
- Pages
- e2528-e2528
- ISSN
- 2041-4889 (ISSN-L)
- PMC
- PMC5386361
- OpenAlex
- W2570059429
- Cited by
- 7
- References
- 74
- Citations by year
- 2019: 2 · 2018: 3 · 2017: 2
- FWCI
- 0.78
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Cell Death and Disease (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Association pour la Recherche sur le Cancer (ANR-10-INSB-04)
- Agence Nationale de la Recherche (ANR-10–INSB–04, ANR-10–INSB–04-01, 10-INSB-04-01, ANR-10, ANR-10-INBS-0004)
- Institut National de la Santé et de la Recherche Médicale
- Centre National de la Recherche Scientifique
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Physical Sciences › Engineering › Biomedical Engineering › Slime Mold and Myxomycetes Research
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- developmental cell death, Dictyostelium, vacuolization, rRNA synthesis inhibition, DNA synthesis inhibition, starvation, cyclic AMP, c-di-GMP, DIF-1, clumping
- MeSH terms
- Cyclic AMP, Animals, Autophagy, Cell Nucleolus, Dictyostelium, Vacuoles, Protozoan Proteins, Cell Death, Apoptosis
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