Cited by 94Genomics and Chromatin DynamicsNuclear Structure and FunctionEpigenetics and DNA Methylation

Epigenetic regulation of TTF-I-mediated promoter-terminator interactions of rRNA genes

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Németh, Attila, Guibert, Sylvain, Tiwari, Vijay Kumar, Ohlsson, Rolf, Längst, Gernot·2008·The EMBO Journal

Abstract

Ribosomal RNA synthesis is the eukaryotic cell's main transcriptional activity, but little is known about the chromatin domain organization and epigenetics of actively transcribed rRNA genes. Here, we show epigenetic and spatial organization of mouse rRNA genes at the molecular level. TTF-I-binding sites subdivide the rRNA transcription unit into functional chromatin domains and sharply delimit transcription factor occupancy. H2A.Z-containing nucleosomes occupy the spacer promoter next to a newly characterized TTF-I-binding site. The spacer and the promoter proximal TTF-I-binding sites demarcate the enhancer. DNA from both the enhancer and the coding region is hypomethylated in actively transcribed repeats. 3C analysis revealed an interaction between promoter and terminator regions, which brings the beginning and end of active rRNA genes into close contact. Reporter assays show that TTF-I mediates this interaction, thereby linking topology and epigenetic regulation of the rRNA genes.

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Crossref · 10.1038/emboj.2008.57

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Authors
Németh, A., Guibert, S., Tiwari, V. K., Ohlsson, R., & Längst, G.
Affiliations
  • University of Regensburg (DE)
  • Uppsala University (SE)
Corresponding author
Längst, Gernot
Venue
The EMBO Journal
Published
2008-03-20
Volume
27
Issue
8
Pages
1255-1265
ISSN
  • 0261-4189 (ISSN-L)
  • 1460-2075
OpenAlex
W2045240569
Cited by
94
References
39
Citations by year
2026: 1 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 5 · 2021: 4 · 2020: 4 · 2019: 5 · 2018: 4 · 2017: 4 · 2016: 7 · 2015: 5 · 2014: 6 · 2013: 9 · 2012: 13
FWCI
3.02
Type
journal article
Language
en
Open access
Locations
  • The EMBO Journal (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • University of Regensburg Publication Server (University of Regensburg) (submittedVersion)
  • Research Portal (Queen's University Belfast) (submittedVersion)
Funders
Deutsche Forschungsgemeinschaft
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
TATA-binding protein, ribosomal DNA, rDNA transcription, epigenetic regulation, chromatin domains, DNA methylation, enhancers, chromatin organization, 3D genome organization, mice
MeSH terms
Animals, Binding, Competitive, Cricetulus, DNA-Binding Proteins, Cricetinae, Promoter Regions, Genetic, Protein Binding, Terminator Regions, Genetic, Transcription Factors, Transcription, Genetic, CHO Cells, Genes, rRNA, NIH 3T3 Cells, Epigenesis, Genetic, Mice

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