Fast transcription rates of RNA polymerase II in human cells
Open accessAbstract
Averaged estimates of RNA polymerase II (RNAPII) elongation rates in mammalian cells have been shown to range between 1.3 and 4.3 kb min(-1). In this work, nascent RNAs from an integrated human immunodeficiency virus type 1-derived vector were detectable at the single living cell level by fluorescent RNA tagging. At steady state, a constant number of RNAs was measured corresponding to a minimal density of polymerases with negligible fluctuations over time. Recovery of fluorescence after photobleaching was complete within seconds, indicating a high rate of RNA biogenesis. The calculated transcription rate above 50 kb min(-1) points towards a wide dynamic range of RNAPII velocities in living cells.
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Maiuri et al reported an extremely high RNA Pol II speed (about 80 kb/min) on an HIV-based vector integrated in one copy, whereas when integrated in 35 copies, RNA Pol II velocity was estimated at 1.6 kb/min.
“Along the same line, Maiuri et al (2011) even reported an extremely high RNA Pol II speed (about 80 kb/min) on an HIV‐based vector integrated in one copy, whereas when integrated in 35 copies, RNA Pol II velocity was estimated at 1.6 kb/min, similar to earlier estimates from multicopy HIV‐derived vector insertions (Boireau et al, 2007).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Maiuri et al, 2011
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Cites
A guided tour into subcellular colocalization analysis in light microscopy
Bolte, S., & Cordelières, F. (2006) · Journal of Microscopy · doi:10.1111/j.1365-2818.2006.01706.x · Cited by 5,238 · see citing works
Stochastic mRNA Synthesis in Mammalian Cells
Raj, A., Peskin, C. S., Tranchina, D., Vargas, D. Y., & Tyagi, S. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040309 · Cited by 1,737 · see citing works
Localization of ASH1 mRNA particles in living yeast.
Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S., Singer, R., & Long, R. M. (1998) · Molecules and Cells · doi:10.1016/s1097-2765(00)80143-4 · Cited by 1,597 · see citing works
Visualization of Single RNA Transcripts in Situ
Femino, A. M., Fay, F. S., Fogarty, K., & Singer, R. H. (1998) · Science · doi:10.1126/science.280.5363.585 · Cited by 1,504 · see citing works
Real-Time Kinetics of Gene Activity in Individual Bacteria
Golding, I., Paulsson, J., Zawilski, S. M., & Cox, E. C. (2005) · Cell · doi:10.1016/j.cell.2005.09.031 · Cited by 1,468 · see citing works
High mobility of proteins in the mammalian cell nucleus
Phair, R. D., & Misteli, T. (2000) · Nature · doi:10.1038/35007077 · Cited by 1,212 · see citing works
Force and velocity measured for single molecules of RNA polymerase.
Wang, M. D., Schnitzer, M., Yin, H., Landick, R., Gelles, J., & Block, S. (1998) · Science · doi:10.1126/science.282.5390.902 · Cited by 894 · see citing works
Transcriptional Pulsing of a Developmental Gene
Chubb, J. R., Trcek, T., Shenoy, S. M., & Singer, R. H. (2006) · Current Biology · doi:10.1016/j.cub.2006.03.092 · Cited by 714 · see citing works
From Silencing to Gene Expression
Janicki, S. M., Tsukamoto, T., Salghetti, S. E., Tansey, W. P., Sachidanandam, R., Prasanth, K. V., Ried, T., Shav-Tal, Y., Bertrand, E., Singer, R. H., et al. (2004) · Cell · doi:10.1016/s0092-8674(04)00171-0 · Cited by 704 · see citing works
In vivo dynamics of RNA polymerase II transcription
Darzacq, X., Shav-Tal, Y., de Turris, V., Brody, Y., Shenoy, S. M., Phair, R. D., & Singer, R. H. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1280 · Cited by 665 · see citing works
Analysis of Binding Reactions by Fluorescence Recovery after Photobleaching
Sprague, B. L., Pego, R. L., Stavreva, D. A., & McNally, J. G. (2004) · Biophysical Journal · doi:10.1529/biophysj.103.026765 · Cited by 617 · see citing works
Self-citationTranscription Against an Applied Force
Yin, H. Y., Wang, M. D., Svoboda, K., Landick, R. C., Block, S. M., & Gelles, J. (1995) · Science · doi:10.1126/science.270.5242.1653 · Cited by 592 · see citing works
Single mRNA Molecules Demonstrate Probabilistic Movement in Living Mammalian Cells
Fusco, D. N., Accornero, N., Lavoie, B. A., Shenoy, S. M., Blanchard, J., Singer, R. H., & Bertrand, É. (2003) · Current Biology · doi:10.1016/s0960-9822(02)01436-7 · Cited by 583 · see citing works
Dynamics of Single mRNPs in Nuclei of Living Cells
Shav‐Tal, Y., Darzacq, X., Shenoy, S. M., Fusco, D. N., Janicki, S. M., Spector, D. L., & Singer, R. H. (2004) · Science · doi:10.1126/science.1099754 · Cited by 494 · see citing works
Rates of in situ transcription and splicing in large human genes
Singh, J., & Padgett, R. A. (2009) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1666 · Cited by 487 · see citing works
Sage, D., Neumann, F. R., Hediger, F., Gasser, S. M., & Unser, M. A. (2005) · IEEE Transactions on Image Processing · doi:10.1109/tip.2005.852787 · Cited by 456 · see citing works
A Kinetic Framework for a Mammalian RNA Polymerase in Vivo
Dundr, M., Hoffmann‐Rohrer, U., Hu, Q., Grummt, I., Rothblum, L. I., Phair, R. D., & Misteli, T. (2002) · Science · doi:10.1126/science.1076164 · Cited by 436 · see citing works
Forrest, K. M., & Gavis, E. R. (2003) · Current Biology · doi:10.1016/s0960-9822(03)00451-2 · Cited by 419 · see citing works
A transgenic mouse for in vivo detection of endogenous labeled mRNA
Lionnet, T., Czaplinski, K., Darzacq, X., Shav-Tal, Y., Wells, A. L., Chao, J., Park, H., de Turris, V., Lopez-Jones, M., & Singer, R. (2011) · Nature Methods · doi:10.1038/nmeth.1551 · Cited by 372 · see citing works
The human dystrophin gene requires 16 hours to be transcribed and is cotranscriptionally spliced
Tennyson, C. N., Klamut, H. J., & Worton, R. G. (1995) · Nature Genetics · doi:10.1038/ng0295-184 · Cited by 369 · see citing works
Progression of the cell cycle through mitosis leads to abortion of nascent transcripts
Shermoen, A. W., & O'Farrell, P. H. (1991) · Cell · doi:10.1016/0092-8674(91)90182-x · Cited by 360 · see citing works
Processive translocation and DNA unwinding by individual RecBCD enzyme molecules
Bianco, P. R., Brewer, L. R., Corzett, M., et al. (2001) · Nature · doi:10.1038/35053131 · Cited by 347 · see citing works
Extensions of DAMAS and Benefits and Limitations of Deconvolution in Beamforming
Dougherty, R. P. (2005) · AIAA/CEAS Aeroacoustics Conference · doi:10.2514/6.2005-2961 · Cited by 338 · see citing works
Transcription by single molecules of RNA polymerase observed by light microscopy
Schafer, D. A., Gelles, J., Sheetz, M. P., & Landick, R. C. (1991) · Nature · doi:10.1038/352444a0 · Cited by 295 · see citing works
RNA dynamics in live <i>Escherichia coli</i> cells
Golding, I., & Cox, E. C. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0404443101 · Cited by 288 · see citing works
Backtracking determines the force sensitivity of RNAP II in a factor-dependent manner
Galburt, E. A., Grill, S. W., Wiedmann, A., Lubkowska, L., Choy, J., Nogales, E., Kashlev, M., & Bustamante, C. (2007) · Nature · doi:10.1038/nature05701 · Cited by 284 · see citing works
Lušić, M., Marcello, A., Cereseto, A., & Giacca, M. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg631 · Cited by 250 · see citing works
Self-citationThe transcriptional cycle of HIV-1 in real-time and live cells
Boireau, S., Maiuri, P., Basyuk, E., de la Mata, M., Knezevich, A., Pradet-Balade, B., Bäcker, V., Kornblihtt, A., Marcello, A., & Bertrand, E. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200706018 · Cited by 191 · see citing works
Self-citationSingle-allele analysis of transcription kinetics in living mammalian cells
Yunger, S., Rosenfeld, L., Garini, Y., & Shav-Tal, Y. (2010) · Nature Methods · doi:10.1038/nmeth.1482 · Cited by 170 · see citing works
Mueller, F., Wach, P., & McNally, J. G. (2008) · Biophysical Journal · doi:10.1529/biophysj.107.123182 · Cited by 166 · see citing works
Self-citationRapid Changes in Drosophila Transcription After an Instantaneous Heat Shock
O’Brien, T., & Lis, J. T. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.6.3456 · Cited by 163 · see citing works
A wave of nascent transcription on activated human genes
Wada, Y., Ohta, Y., Xu, M., Tsutsumi, S., Minami, T., Inoue, K., Komura, D., Kitakami, J.-I., Oshida, N., Papantonis, A., et al. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0902573106 · Cited by 152 · see citing works
Fast, DNA‐sequence independent translocation by FtsK in a single‐molecule experiment
Saleh, O. A., Pérals, C., Barre, F., & Allemand, J. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600242 · Cited by 138 · see citing works
Latency: the hidden HIV-1 challenge
Marcello, A. (2006) · Retrovirology · doi:10.1186/1742-4690-3-7 · Cited by 126 · see citing works
Self-citationTracking rates of transcription and splicing in vivo
Ardehali, M. B., & Lis, J. T. (2009) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1109-1123 · Cited by 125 · see citing works
A thermodynamic analysis of the sequence-specific binding of RNA by bacteriophage MS2 coat protein
Johansson, H. E., Dertinger, D., LeCuyer, K. A., et al. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.16.9244 · Cited by 104 · see citing works
Sehgal, P. B., Derman, E., Molloy, G. R., Tamm, I., & Darnell, J. E., Jr. (1976) · Science · doi:10.1126/science.982026 · Cited by 101 · see citing works
Multiple Modes of Transcriptional Regulation by the HIV‐1 Tat Transactivator
Marcello, A., Zoppè, M., & Giacca, M. (2001) · IUBMB Life · doi:10.1080/152165401753544241 · Cited by 89 · see citing works
Self-citationReal-time RNA profiling within a single bacterium
Le, T. T., Harlepp, S., Guet, C. C., Dittmar, K. A., Emonet, T., Pan, T., & Cluzel, P. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0503311102 · Cited by 88 · see citing works
Kula, A., Guerra, J., Knezevich, A., Kleva, D., Myers, M., & Marcello, A. (2011) · Retrovirology · doi:10.1186/1742-4690-8-60 · Cited by 82 · see citing works
Self-citationTranscriptional competence of the integrated HIV‐1 provirus at the nuclear periphery
Dieudonné, M., Maiuri, P., Biancotto, C., Knezevich, A., Kula, A., Lušić, M., & Marcello, A. (2009) · The EMBO Journal · doi:10.1038/emboj.2009.141 · Cited by 73 · see citing works
Self-citationA real-time view of the TAR:Tat:P-TEFb complex at HIV-1 transcription sites
Molle, D., Maiuri, P., Boireau, S., Bertrand, É., Knezevich, A., Marcello, A., & Basyuk, E. I. (2007) · Retrovirology · doi:10.1186/1742-4690-4-36 · Cited by 56 · see citing works
Self-citationWang, F., Koyama, N., Nishida, H., et al. (2006) · Molecular and Cellular Biology · doi:10.1128/mcb.02189-05 · Cited by 50 · see citing works
Real-time imaging of the HIV-1 transcription cycle in single living cells
Maiuri, P., Knezevich, A., Bertrand, É., & Marcello, A. (2010) · Methods · doi:10.1016/j.ymeth.2010.06.015 · Cited by 29 · see citing works
Self-citationde Marco, A., Biancotto, C., Knezevich, A., Maiuri, P., Vardabasso, C., & Marcello, A. (2008) · Retrovirology · doi:10.1186/1742-4690-5-98 · Cited by 23 · see citing works
Self-citationNuclear positional control of HIV transcription in 4D
Marcello, A., Dhir, S., & Dieudonné, M. (2010) · Nucleus · doi:10.4161/nucl.1.1.10136 · Cited by 17 · see citing works
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RNA polymerase II speed: a key player in controlling and adapting transcriptome composition
Muniz, L., Nicolas, E., & Trouche, D. (2021) · The EMBO Journal · doi:10.15252/embj.2020105740 · Cited by 203 · see citing works
“…recovery after photobleaching (FRAP) experiments using MS2 fluorescent proteins to label nascent MS2 repeat-containing RNAs (Boireau et al , 2007; Darzacq et al , 2007; Ben-Ari et al , 2010; Yunger et al , 2010; Brody et al , 2011; Maiuri et al , 2011; Muramoto et al , 2012; Corrigan et al , 2016).”
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Da, L.-T., Pardo Avila, F., Wang, D., & Huang, X. (2013) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1003020 · Cited by 54 · see citing works
Annibale, P., & Gratton, E. (2015) · Scientific Reports · doi:10.1038/srep09258 · Cited by 24 · see citing works
Cell-cycle dependence of transcription dominates noise in gene expression
Zopf, C. J., Quinn, K., Zeidman, J., & Maheshri, N. (2013) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1003161 · Cited by 4 · see citing works
- Authors
- Maiuri, P., Knezevich, A., De Marco, A., Mazza, D., Kula, A., McNally, J. G., & Marcello, A.
- Affiliations
- International Centre for Genetic Engineering and Biotechnology (IT)
- National Institutes of Health (US)
- Corresponding author
- Maiuri, Paolo
- Marcello, Alessandro
- Venue
- The EMBO Reports
- Published
- 2011-10-21
- Volume
- 12
- Issue
- 12
- Pages
- 1280-1285
- ISSN
- 1469-221X (ISSN-L)
- 1469-3178
- PMC
- PMC3245692
- OpenAlex
- W2071759249
- Cited by
- 91
- References
- 46
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 5 · 2021: 4 · 2020: 4 · 2019: 6 · 2018: 6 · 2017: 5 · 2016: 5 · 2015: 6 · 2014: 6 · 2013: 13 · 2012: 18
- FWCI
- 5.03
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- EMBO Reports (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Archivio Istituzionale della Ricerca - Università Vita-Salute San Raffaele (submittedVersion)
- Funders
- Beneficentia Stiftung
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- RNA polymerase II, nascent RNA, FRAP, HIV-1 vectors, RNA biogenesis
- MeSH terms
- Cell Line, Cell Survival, Gene Expression Regulation, Humans, Retroviridae, RNA Polymerase II, RNA, Messenger, Transcription, Genetic, In Situ Hybridization, Fluorescence Recovery After Photobleaching
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