Cited by 176Gene Regulatory Network AnalysisGenomics and Chromatin DynamicsAdvanced Fluorescence Microscopy Techniques

Single-allele analysis of transcription kinetics in living mammalian cells

Yunger, Sharon, Rosenfeld, Liat, Garini, Yuval, Shav-Tal, Yaron·2010·Nature Methods

Abstract

We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.

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Crossref · 10.1038/nmeth.1482

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  • Single integration of the CCND1 gene under the control of an endogenous or viral promoter led to an RNA Pol II speed of 0.31-0.78 kb/min.

    “Nevertheless, single integration of the CCND1 gene under the control of an endogenous or viral promoter led to an RNA Pol II speed of 0.31–0.78 kb/min (Yunger et al, 2010) and the number of gene units in the tandem array had no effect on RNA Pol II speed in another study (Brody et al, 2011).”

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Authors
Yunger, S., Rosenfeld, L., Garini, Y., & Shav-Tal, Y.
Affiliations
Bar-Ilan University (IL)
Venue
Nature Methods
Published
2010-07-18
Volume
7
Issue
8
Pages
631-633
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W2069280583
Cited by
170
References
21
Citations by year
2026: 3 · 2025: 7 · 2024: 8 · 2023: 5 · 2022: 7 · 2021: 4 · 2020: 12 · 2019: 14 · 2018: 3 · 2017: 16 · 2016: 17 · 2015: 12 · 2014: 10 · 2013: 19 · 2012: 15
FWCI
6.77
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Methods (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
Keywords
transcription kinetics, transcriptional bursting, cyclin D1, cell cycle-dependent transcription, DNA replication, live-cell imaging, viral promoters
MeSH terms
Alleles, DNA Replication, Humans, Kinetics, Transcription, Genetic, Cyclin D1

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