Single-allele analysis of transcription kinetics in living mammalian cells
Abstract
We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.
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Single integration of the CCND1 gene under the control of an endogenous or viral promoter led to an RNA Pol II speed of 0.31-0.78 kb/min.
“Nevertheless, single integration of the CCND1 gene under the control of an endogenous or viral promoter led to an RNA Pol II speed of 0.31–0.78 kb/min (Yunger et al, 2010) and the number of gene units in the tandem array had no effect on RNA Pol II speed in another study (Brody et al, 2011).”
From RNA polymerase II speed: a key player in controlling and adapting transcriptome composition (2021) · cites this paper as Yunger et al, 2010
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- Authors
- Yunger, S., Rosenfeld, L., Garini, Y., & Shav-Tal, Y.
- Affiliations
- Bar-Ilan University (IL)
- ORCID
- Garini, Yuval
- Venue
- Nature Methods
- Published
- 2010-07-18
- Volume
- 7
- Issue
- 8
- Pages
- 631-633
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2069280583
- Cited by
- 170
- References
- 21
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 8 · 2023: 5 · 2022: 7 · 2021: 4 · 2020: 12 · 2019: 14 · 2018: 3 · 2017: 16 · 2016: 17 · 2015: 12 · 2014: 10 · 2013: 19 · 2012: 15
- FWCI
- 6.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Methods (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gene Regulatory Network Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- transcription kinetics, transcriptional bursting, cyclin D1, cell cycle-dependent transcription, DNA replication, live-cell imaging, viral promoters
- MeSH terms
- Alleles, DNA Replication, Humans, Kinetics, Transcription, Genetic, Cyclin D1
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