CoChat
Sign Up
Cited by 78Alzheimer's disease research and treatmentsNeuroinflammation and Neurodegeneration MechanismsDementia and Cognitive Impairment Research

Identification of amyloid β-peptide responsive genes by cDNA microarray technology: Involvement of RTP801 in amyloid β-peptide toxicity

Open access
Kim, Jae-Ryong, Lee, Seung-Rock, Chung, Hyun Jin, Kim, Seongyong, Baek, Suk-Hwan, Kim, Jung Hye, Kim, Yong-Sun·2003·Experimental & Molecular Medicine
DOI 10.1038/emm.2003.53
Open this paper in CoChat — free accountOpen PDF

Abstract

Amyloid beta-peptide (Abeta), a causative molecule in the pathogenesis of Alzheimer's disease and the main component of senile plaques, is known to be neurotoxic in vitro and in vivo. The mechanisms involved in this Ab-mediated neurotoxicity are not fully understood, although there is evidence to suggest the involvement of oxidative stress, alterations in calcium homeostasis, and/or of CDK activators. Many studies have suggested that Ab may exert its toxic effect via the activation of transcription factors. Therefore, we investigated Ab- responsive genes in human neuroblastoma CHP134 cells using 3.1K human DNA microarrays. Among the several genes overexpressed or repressed by Ab, RTP801, Hi95/sestrin 2, and stanniocalcin 2 were confirmed to be Ab-mediated overexpression in the cells by semiquantitative RT-PCR. Transient expression of the sense RTP801 gene in CHP134 cells increased sensitivity to Abeta cytotoxicity and the expression of the antisense RTP801 gene protected the cells from the Abeta toxicity. These results suggest that RTP801 might play important roles in Abeta toxicity and the pathogenesis of Alzheimer's disease.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/emm.2003.53

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 36

  1. Mol Neurosci 1998;10:181-92
  2. Behl C, Davis JB, Lesley R, Schubert D. Hydrogen peroxide mediates amyloid β protein toxicity. Cell 1994;77:817-27
  3. Bouras T, Southey MC, Chang AC, Reddel RR, Willhite D, Glynne R, Henderson MA, Armes JE, Venter DJ. Stannio- calcin 2 is an estrogen-responsive gene coexpressed with the estrogen receptor in human breast cancer. Cancer Res 2002;62:1289-95
  4. Budanov AV, Shoshani T, Faerman A, Zelin E, Kamer I, Kalinski H, Gorodin S, Fishman A, Chajut A, Einat P, Skaliter R, Gudkov AV, Chumakov PM, Feinstein E. Identification of a novel stress-responsive gene Hi95 involved in regulation of cell viability. Oncogene 2002;21:6017-31
  5. Butterfield DA, Hensley K, Harris M, Mattson M, Carney J. β-Amyloid peptide free radical fragments initiate synapto- somal lipoperoxidation in a sequence-specific fashion: impli- cations to Alzheimer's disease. Biochem Biophys Res Com- mun 1994;200:710-5
  6. Chomczynski P, Sacchi N. Single-step method of RNA isola- tion by acid guanidinium thiocyanate-phenol-chloroform extra- ction. Anal Biochem 1987;162:156-9
  7. Green KN, Boyle JP, Peers C. Hypoxia potentiates exocyto- sis and Ca 2+ channels in PC12 cells via increased amyloid β peptide formation and reactive oxygen species generation. J Physiol 2002;541:1013-23
  8. Greeve I, Hermans-Borgmeyer I, Brellinger C, Kasper D, Gomez-Isla T, Behl C, Levkau B, Nitsch RM. The human DIMINUTO/DWARF1 homolog seladin-1 confers resistance to Alzheimer's disease-associated neurodegeneration and oxi- dative stress. J Neurosci 2000;20:7345-52
  9. Hata R, Masumura M, Akatsu H, Li F, Fujita H, Nagai Y, Yamamoto T, Okada H, Kosaka K, Sakanaka M, Sawada T. Up-regulation of calcineurin Aβ mRNA in the Alzheimer's disease brain: assessment by cDNA microarray. Biochem Biophys Res Commun 2001;284:310-6
  10. Huang X, Cuajungco MP, Atwood CS, Hartshorn MA, Tyndall JD, Hanson GR, Stokes KC, Leopold M, Multhaup G, Gold- stein LE, Scarpa RC, Saunders AJ, Lim J, Moir RD, Glabe C, Bowden EF, Masters CL, Fairlie DP, Tanzi RE, Bush AI. Cu (II) potentiation of Alzheimer Aβ neurotoxicity: Correlation with cell-free hydrogen peroxide production and metal reduction. J Biol Chem 1999;74:37111-6
  11. Ishibashi K, Miyamoto K, Taketani Y, Morita K, Takeda E, Sasaki S, Imai M. Molecular cloning of a second human stanniocalcin homologue (STC2). Biochem Biophys Res Com- mun 1998;250:252-8
  12. Jang JH, Surh YJ. β-Amyloid induces oxidative DNA damage and cell death through activation of c-Jun N terminal kinase. Ann N Y Acad Sci 2002;973:228-36
  13. Jellinek DA, Chang AC, Larsen MR, Wang X, Robinson PJ, Reddel RR. Stanniocalcin 1 and 2 are secreted as phos- phoproteins from human fibrosarcoma cells. Biochem J 2000; 350:2453-61
  14. Kaltschmidt B, Uherek M, Wellmann H, Volk B, Kaltschmidt C. Inhibition of NF-κB potentiates amyloid β-mediated neuro- nal apoptosis. Proc Natl Acad Sci USA 1999;96:9409-14
  15. Lovell MA, Gabbita SP, Markesbery WR. Increased DNA oxidation and decreased levels of repair products in Al- zheimer's disease ventricular CSF. J Neurochem 1999;72: 771-6
  16. Maccioni RB, Otth C, Concha II, Munoz JP. The protein kinase Cdk5. Structural aspects, roles in neurogenesis and involvement in Alzheimer's pathology. Eur J Biochem 2001; 268:1518-27
  17. Marcus DL, Strafaci JA, Miller DC, Masia S, Thomas CG, Rosman J, Hussain S, Freedman ML. Quantitative neuronal c-fos and c-jun expression in Alzheimer's disease. Neurobiol Aging 1998;19:393-400
  18. Mark RJ, Blanc EM, Mattson MP. Amyloid β-peptide and oxidative cellular injury in Alzheimer's disease. Mol Neurobiol 1996;12:211-24
  19. Markesbery WR. Oxidative stress hypothesis in Alzheimer's disease. Free Radic Biol Med 1997;23:134-47
  20. Mattson MP, Guo Q, Furukawa K, Pedersen WA. Presenilins, the endoplasmic reticulum, and neuronal apoptosis in Alzhei- mer's disease. J Neurochem 1998;70:1-14
  21. Miranda S, Opazo C, Larrondo LF, Munoz FJ, Ruiz F, Leigh- ton F, Inestrosa NC. The role of oxidative stress in the toxicity induced by amyloid β-peptide in Alzheimer's disease. Prog Neurobiol 2000;62:633-48
  22. Moore EE, Kuestner RE, Conklin DC, Whitmore TE, Downey W, Buddle MM, Adams RL, Bell LA, Thompson DL, Wolf A, Chen L, Stamm MR, Grant FJ, Lok S, Ren H, De Jongh KS. Stanniocalcin 2: characterization of the protein and its localization to human pancreatic cells. Horm Metab Res 1999;31:406-14
  23. Noh YH, Kim JA, Lim GR, Ro YT, Koo JH, Lee YS, Han DS, Park HK, Ahn MJ. Detection of circulating tumor cells in patients with gastrointestinal tract cancer using RT-PCR and its clinical implications. Exp Mol Med 2001;33:8-14
  24. Pappolla MA, Omar RA, Kim KS, Robakis NK. Immuno- histochemical evidence of oxidative stress in Alzheimer's disease. Am J Pathol 1992;140:621-8
  25. Paradis E, Douillard H, Koutroumanis M, Goodyer C, Le- Blanc A. Amyloid β peptide of Alzheimer's disease down- regulates Bcl-2 and upregulates bax expression in human neurons. J Neurosci 1996;16:7533-9
  26. Park GH, Choe J, Choo HJ, Park YG, Sohn J, Kim MK. Genome-wide expression profiling of 8-chloroadenosine-and 8-chloro-cAMP-treated human neuroblastoma cells using radioactive human cDNA microarray. Exp Mol Med 2002;34: 184-93
  27. Peeters H, Debeer P, Bairoch A, Wilquet V, Huysmans C, Parthoens E, Fryns JP, Gewillig M, Nakamura Y, Niikawa N, Van De Ven W, Devriendt K. PA26 is a candidate gene for heterotaxia in humans: identification of a novel PA26- related gene family in human and mouse. Hum Genet 2003; 112:573-80.
  28. Santiard-Baron D, Gosset P, Nicole A, Sinet PM, Christen Y, Ceballos-Picot I. Identification of β-amyloid-responsive ge- nes by RNA differential display: early induction of a DNA damage-inducible gene, gadd45. Exp Neurol 1999;158:206- 13
  29. Schippling S, Kontush A, Arlt S, Buhmann C, Sturenburg HJ, Mann U, Muller-Thomsen T, Beisiegel U. Increased lipo- protein oxidation in Alzheimer's disease. Free Radic Biol Med 2000;28:351-60
  30. Shoshani T, Faerman A, Mett I, Zelin E, Tenne T, Gorodin S, Moshel Y, Elbaz S, Budanov A, Chajut A, Kalinski H, Kamer I, Rozen A, Mor O, Keshet E, Leshkowitz D, Einat P, Skaliter R, Feinstein E. Identification of a novel hypoxia- inducible factor 1-responsive gene, RTP801, involved in apoptosis. Mol Cell Biol 2002;22:2283-93
  31. Smith CD, Carney JM, Starke-Reed PE, Oliver CN, Stadtman ER, Floyd RA, Markesbery WR. Excess brain protein oxi- dation and enzyme dysfunction in normal aging and in Alzheimer disease. Proc Natl Acad Sci USA 1991;88:10540-3
  32. Suh SW, Jensen KB, Jensen MS, Silva DS, Kesslak PJ, Danscher G, Frederickson CJ. Histochemically-reactive zinc in amyloid plaques, angiopathy, and degenerating neurons of Alzheimer's diseased brains. Brain Res 2000;852:274-8
  33. Sundell K, Bjornsson BT, Itoh H, Kawauchi H. Chum salmon (Oncorhynchus keta) stanniocalcin inhibits in vitro intestinal calcium uptake in Atlantic cod (Gadus morhua). J Comp Physiol [B] 1992;162:489-95
  34. Thompson CM, Markesbery WR, Ehmann WD, Mao YX, Vance DE. Regional brain trace-element studies in Alzhei- mer's disease. Neurotoxicology 1988;9:1-7
  35. Vitek MP, Bhattacharya K, Glendening JM, Stopa E, Vlas- sara H, Bucala R, Manogue K, Cerami A. Advanced glyca- tion end products contribute to amyloidosis in Alzheimer disease. Proc Natl Acad Sci USA 1994;91:4766-70
  36. Walker DG, Lue LF, Beach TG. Gene expression profiling of amyloid β peptide-stimulated human post-mortem brain microglia. Neurobiol Aging 2001;22:957-66

Cited by

Sorted by citations

Authors
Kim, J.-R., Lee, S.-R., Chung, H. J., Kim, S., Baek, S.-H., Kim, J. H., & Kim, Y.-S.
Affiliations
  • Yeungnam University College (KR)
  • Yeungnam University (KR)
Corresponding author
  • Kim, Jung Hye
  • Kim, Yong-Sun
ORCID
Venue
Experimental & Molecular Medicine
Publisher
Springer Science and Business Media LLC
Published
2003-10-01
Volume
35
Issue
5
Pages
403-411
ISSN
  • 1226-3613 (ISSN-L)
  • 2092-6413
DOI
10.1038/emm.2003.53
OpenAlex
W2081572505
Cited by
78
References
1
Citations by year
2026: 2 · 2025: 3 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 3 · 2019: 5 · 2018: 3 · 2016: 2 · 2015: 2 · 2014: 2 · 2013: 2 · 2012: 3
FWCI
3.65
Type
journal article
Language
en
Open access
Licence
Locations
  • Experimental & Molecular Medicine (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
Keywords
amyloid-beta, Alzheimer's disease, RTP801, STC2, gene expression profiling, transcriptional response, antisense gene expression, neuroblastoma cells, amyloid plaques
MeSH terms
Sestrins, Alzheimer Disease, Base Sequence, Gene Expression Regulation, Glycoproteins, Humans, Molecular Sequence Data, Nuclear Proteins, RNA, Messenger, Transcription Factors, Amyloid beta-Peptides, DNA, Complementary, Reverse Transcriptase Polymerase Chain Reaction, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Intercellular Signaling Peptides and Proteins, Cell Line, Tumor

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review