The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Butyrate-producing Clostridium cluster XIVa species specifically colonize mucins in an in vitro gut model
Open accessAbstract
The human gut is colonized by a complex microbiota with multiple benefits. Although the surface-attached, mucosal microbiota has a unique composition and potential to influence human health, it remains difficult to study in vivo. Therefore, we performed an in-depth microbial characterization (human intestinal tract chip (HITChip)) of a recently developed dynamic in vitro gut model, which simulates both luminal and mucosal gut microbes (mucosal-simulator of human intestinal microbial ecosystem (M-SHIME)). Inter-individual differences among human subjects were confirmed and microbial patterns unique for each individual were preserved in vitro. Furthermore, in correspondence with in vivo studies, Bacteroidetes and Proteobacteria were enriched in the luminal content while Firmicutes rather colonized the mucin layer, with Clostridium cluster XIVa accounting for almost 60% of the mucin-adhered microbiota. Of the many acetate and/or lactate-converting butyrate producers within this cluster, Roseburia intestinalis and Eubacterium rectale most specifically colonized mucins. These 16S rRNA gene-based results were confirmed at a functional level as butyryl-CoA:acetate-CoA transferase gene sequences belonged to different species in the luminal as opposed to the mucin-adhered microbiota, with Roseburia species governing the mucosal butyrate production. Correspondingly, the simulated mucosal environment induced a shift from acetate towards butyrate. As not only inter-individual differences were preserved but also because compared with conventional models, washout of relevant mucin-adhered microbes was avoided, simulating the mucosal gut microbiota represents a breakthrough in modeling and mechanistically studying the human intestinal microbiome in health and disease. Finally, as mucosal butyrate producers produce butyrate close to the epithelium, they may enhance butyrate bioavailability, which could be useful in treating diseases, such as inflammatory bowel disease.
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Cites
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“As colonization of mucus by different mucolytic species may be crucial with respect to human health (Png et al., 2010), the M-SHIME may be improved by adding a distal colon region.”
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Introduction to the human gut microbiota
Thursby, E., & Juge, N. (2017) · Biochemical Journal · doi:10.1042/bcj20160510 · Cited by 3,600
Bile acid-microbiota crosstalk in gastrointestinal inflammation and carcinogenesis
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Probiotics, gut microbiota, and their influence on host health and disease
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The neuropharmacology of butyrate: the bread and butter of the microbiota-gut-brain axis
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Metadata
- Authors
- Van den Abbeele, P., Belzer, C., Goossens, M., Kleerebezem, M., De Vos, W. M., Thas, O., De Weirdt, R., Kerckhof, F.-M., & Van de Wiele, T.
- Venue
- The ISME Journal
- Publisher
- Oxford University Press (OUP)
- Published
- 2012-12-13
- Volume
- 7
- Issue
- 5
- Pages
- 949-961
- ISSN
- 1751-7362 (ISSN-L)
- 1751-7370
- OpenAlex
- W2051312690
- Cited by
- 638
- References
- 11
- FWCI
- 6.04
- Type
- journal article
- Language
- en
- Open access
- bronze
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