Selective colonization of insoluble substrates by human faecal bacteria

Leitch, E. Carol McWilliam; Walker, Alan W.; Duncan, Sylvia H.; Holtrop, Grietje; Flint, Harry J.

doi:10.1111/j.1462-2920.2006.01186.x

Cited by 297Gut microbiota and healthProbiotics and Fermented FoodsClostridium difficile and Clostridium perfringens research

Selective colonization of insoluble substrates by human faecal bacteria

Leitch, E. Carol McWilliam, Walker, Alan W., Duncan, Sylvia H., Holtrop, Grietje, Flint, Harry J.·2007·Environmental Microbiology

DOI 10.1111/j.1462-2920.2006.01186.x

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Abstract

Insoluble plant polysaccharides and endogenous mucin are important energy sources for human colonic microorganisms. The object of this study was to determine whether or not specific communities colonize these substrates. Using faecal samples from four individuals as inocula for an anaerobic in vitro continuous flow system, the colonization of wheat bran, high amylose starch and porcine gastric mucin was examined. Recovered substrates were extensively washed and the remaining tightly attached bacterial communities were identified using polymerase chain reaction-amplified 16S rRNA gene sequences and fluorescent in situ hybridization. The substrate had a major influence on the species of attached bacteria detected. Sequences retrieved from bran were dominated by clostridial cluster XIVa bacteria, including uncultured relatives of Clostridium hathewayi, Eubacterium rectale and Roseburia species. Bacteroides species were also detected. The most abundant sequences recovered from starch were related to the cultured species Ruminococcus bromii, Bifidobacterium adolescentis, Bifidobacterium breve and E. rectale. The most commonly recovered sequences from mucin were from Bifidobacterium bifidum and uncultured bacteria related to Ruminococcus lactaris. This study suggests that a specific subset of bacteria is likely to be the primary colonizers of particular insoluble colonic substrates. For a given substrate, however, the primary colonizing species may vary between host individuals.

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Crossref · 10.1111/j.1462-2920.2006.01186.x

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These taxa were shown to selectively metabolize specific insoluble carbohydrate substrates, based on in vitro analyses of human fecal samples.
“These taxa were shown to selectively metabolize specific insoluble carbohydrate substrates, based on in vitro analyses of human fecal samples33.”
From Diet and the Intestinal Microbiome: Associations, Functions, and Implications for Health and Disease (2014) · cites this paper as [33]

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Authors: Leitch, E. C. M., Walker, A. W., Duncan, S. H., Holtrop, G., & Flint, H. J.
Affiliations: Wellcome Sanger Institute (GB)
Biomathematics and Statistics Scotland (GB)
Corresponding author: Flint, Harry J.
ORCID: Leitch, E. Carol McWilliam
Walker, Alan W.
Duncan, Sylvia H.
Holtrop, Grietje
Flint, Harry J.
Venue: Environmental Microbiology
Publisher: Wiley
Published: 2006-12-06
Volume: 9
Issue: 3
Pages: 667-679
ISSN: 1462-2912 (ISSN-L)
1462-2920
DOI: 10.1111/j.1462-2920.2006.01186.x
OpenAlex: W2019521764
Cited by: 297
References: 51
Citations by year: 2026: 1 · 2025: 15 · 2024: 16 · 2023: 18 · 2022: 19 · 2021: 25 · 2020: 23 · 2019: 22 · 2018: 15 · 2017: 14 · 2016: 12 · 2015: 11 · 2014: 11 · 2013: 15 · 2012: 17
FWCI: 4.19
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Environmental Microbiology (publishedVersion)
PubMed (publishedVersion)
Funders: Biotechnology and Biological Sciences Research Council
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Health Sciences › Medicine › Infectious Diseases › Clostridium difficile and Clostridium perfringens research
Keywords: insoluble substrates, plant polysaccharides, wheat bran, gastric mucin, Eubacterium rectale, Roseburia, Bifidobacterium, Ruminococcus bromii, Bacteroides, 16S rRNA gene sequencing, fluorescence in situ hybridization, host variation
MeSH terms: Amylose, Bacteria, Bacterial Adhesion, Dietary Fiber, DNA, Bacterial, DNA, Ribosomal, Feces, Gastric Mucins, Humans, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Solubility, Substrate Specificity, Colony Count, Microbial, Polymerase Chain Reaction, In Situ Hybridization, Fluorescence, Genes, rRNA

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Selective colonization of insoluble substrates by human faecal bacteria

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