ALCAM (CD166) is involved in extravasation of monocytes rather than T cells across the blood–brain barrier
Open accessAbstract
Activated leukocyte cell adhesion molecule (ALCAM) has been proposed to mediate leukocyte migration across the blood–brain barrier (BBB) in multiple sclerosis or experimental autoimmune encephalomyelitis (EAE). Here, we confirmed vascular ALCAM expression in human brain tissue samples in situ and on two different human in vitro BBB models. Antibody-mediated inhibition of ALCAM reduced diapedesis of human CD4 + Th1 but not of Th17 cells across the human BBB in vitro. In accordance to human Th1 cells, mouse Th1 cells showed reduced diapedesis across an ALCAM −/− in vitro BBB model under static but no longer under flow conditions. In contrast to the limited role of ALCAM in T cell extravasation across the BBB, we found a contribution of ALCAM to rolling, adhesion, and diapedesis of human CD14 + monocytes across the human BBB under flow and static conditions. Taken together, our study highlights the potential differences in the CNS expression of ALCAM in mouse and human and supports a prominent role for ALCAM in the multi-step extravasation of monocytes across the BBB.
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Activated leukocyte cell adhesion molecule (CD166) can foster diapedesis of monocytes and, to a lesser extent, CD4 T cells during EAE.
“Activated leukocyte cell adhesion molecule (CD166) can also foster diapedesis of monocytes and, to a lesser extent, CD4 T cells during EAE (111, 112).”
From The anatomy and immunology of vasculature in the central nervous system (2019) · cites this paper as [111]
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The anatomy and immunology of vasculature in the central nervous system
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- Authors
- Lyck, R., Lécuyer, M.-A., Abadier, M., Wyss, C. B., Matti, C., Rosito, M., Enzmann, G., Zeis, T., Michel, L., García Martín, A. B., Sallusto, F., Gosselet, F., Deutsch, U., Weiner, J. A., Schaeren-Wiemers, N., Prat, A., & Engelhardt, B.
- Affiliations
- University of Bern (CH)
- Centre Hospitalier de l’Université de Montréal (CA)
- University of Basel (CH)
- Theodor Kocher Institute, University of Bern, Bern, Switzerland
- Institute for Research in Biomedicine (CH)
- Université d'Artois (FR)
- University of Iowa (US)
- Neurobiology, Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland
- Corresponding author
- Lyck, Ruth
- ORCID
- Venue
- Journal of Cerebral Blood Flow & Metabolism
- Publisher
- SAGE Publications
- Published
- 2016-11-14
- Volume
- 37
- Issue
- 8
- Pages
- 2894-2909
- ISSN
- 0271-678X (ISSN-L)
- 1559-7016
- PMC
- PMC5536797
- OpenAlex
- W2551079320
- Cited by
- 75
- References
- 61
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 4 · 2023: 10 · 2022: 17 · 2021: 5 · 2020: 11 · 2019: 7 · 2018: 9 · 2017: 3
- FWCI
- 3.47
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, cc-by-nc)
- Bern Open Repository and Information System (University of Bern) (submittedVersion)
- edoc (University of Basel) (submittedVersion)
- IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (133092)
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Keywords
- ALCAM, CD166, blood-brain barrier, leukocyte transmigration, monocytes, T cells, Th1 cells, Th17 cells, transendothelial migration, leukocyte rolling and adhesion, multiple sclerosis, experimental autoimmune encephalomyelitis, in vitro blood-brain barrier model
- MeSH terms
- Animals, Blood-Brain Barrier, Cells, Cultured, Encephalomyelitis, Autoimmune, Experimental, Endothelium, Vascular, Fetal Proteins, Humans, Mice, Inbred C57BL, Monocytes, Multiple Sclerosis, T-Lymphocytes, Antigens, CD, Cell Adhesion Molecules, Neuronal, Mice, Knockout, Activated-Leukocyte Cell Adhesion Molecule, Endothelial Cells, Transendothelial and Transepithelial Migration
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