Energy demand of synaptic transmission at the hippocampal Schaffer-collateral synapse
Open accessAbstract
Neuroenergetic models of synaptic transmission predicted that energy demand is highest for action potentials (APs) and postsynaptic ion fluxes, whereas the presynaptic contribution is rather small. Here, we addressed the question of energy consumption at Schaffer-collateral synapses. We monitored stimulus-induced changes in extracellular potassium, sodium, and calcium concentration while recording partial oxygen pressure (pO(2)) and NAD(P)H fluorescence. Blockade of postsynaptic receptors reduced ion fluxes as well as pO(2) and NAD(P)H transients by ∼50%. Additional blockade of transmitter release further reduced Na(+), K(+), and pO(2) transients by ∼30% without altering presynaptic APs, indicating considerable contribution of Ca(2+)-removal, transmitter and vesicle turnover to energy consumption.
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In brain slices, [O2] shows a pronounced dip with stimulation, corresponding with increased O2 consumption.
“in brain slices, [O2] shows a pronounced dip with stimulation, corresponding with increased O2 consumption (Hall et al., 2012; Liotta et al., 2012; Ivanov et al., 2014).”
From Fueling thought: Management of glycolysis and oxidative phosphorylation in neuronal metabolism (2018) · cites this paper as Liotta et al., 2012
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- Authors
- Liotta, A., Rösner, J., Huchzermeyer, C., Wojtowicz, A., Kann, O., Schmitz, D., Heinemann, U., & Kovács, R.
- Affiliations
- Charité – Universitätsmedizin Berlin (DE)
- Heidelberg University (DE)
- German Center for Neurodegenerative Diseases (DE)
- Corresponding author
- Heinemann, Uwe
- Venue
- Journal of Cerebral Blood Flow & Metabolism
- Publisher
- SAGE Publications
- Published
- 2012-08-29
- Volume
- 32
- Issue
- 11
- Pages
- 2076-2083
- ISSN
- 0271-678X (ISSN-L)
- 1559-7016
- PMC
- PMC3493999
- OpenAlex
- W1999132438
- Cited by
- 48
- References
- 22
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 5 · 2019: 1 · 2018: 7 · 2017: 3 · 2016: 5 · 2015: 5 · 2014: 5 · 2013: 2 · 2012: 1
- FWCI
- 1.71
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
- DZNE Pub (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Keywords
- synaptic transmission, energy demand, Schaffer collateral synapses, hippocampus, presynaptic action potential, extracellular ion concentration, NAD(P)H fluorescence, neurotransmitter release, calcium removal, neuroenergetics
- MeSH terms
- Action Potentials, Animals, Calcium, Energy Metabolism, gamma-Aminobutyric Acid, Glutamates, Hippocampus, Kinetics, Male, NADP, Synaptic Transmission, Oxygen Consumption, Potassium, Sodium, Synapses, Calcium Channels, Ion Channel Gating, Rats, Wistar, Pyramidal Cells, Excitatory Postsynaptic Potentials, Rats, CA1 Region, Hippocampal, In Vitro Techniques
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