Oxygen Consumption Rates during Three Different Neuronal Activity States in the Hippocampal CA3 Network
Open accessAbstract
The brain is an organ with high metabolic rate. However, little is known about energy utilization during different activity states of neuronal networks. We addressed this issue in area CA3 of hippocampal slice cultures under well-defined recording conditions using a 20% O 2 gas mixture. We combined recordings of local field potential and interstitial partial oxygen pressure (pO 2 ) during three different activity states, namely fast network oscillations in the gamma-frequency band (30 to 100 Hz), spontaneous network activity and absence of spiking (action potentials). Oxygen consumption rates were determined by pO 2 depth profiles with high spatial resolution and a mathematical model that considers convective transport, diffusion, and activity-dependent consumption of oxygen. We show that: (1) Relative oxygen consumption rate during cholinergic gamma oscillations was 2.2-fold and 5.3-fold higher compared with spontaneous activity and absence of spiking, respectively. (2) Gamma oscillations were associated with a similar large decrease in pO 2 as observed previously with a 95% O 2 gas mixture. (3) Sufficient oxygenation during fast network oscillations in vivo is ensured by the calculated critical radius of 30 to 40 μm around a capillary. We conclude that the structural and biophysical features of brain tissue permit variations in local oxygen consumption by a factor of about five.
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The rate of oxygen consumption during hippocampal gamma oscillations can equal the rate during a seizure.
“Studies have shown that the rate of oxygen consumption during hippocampal gamma oscillations can be equivalent to the rate of oxygen spent during a seizure [83,84].”
From Parvalbumin interneuron vulnerability and brain disorders (2021) · cites this paper as [84]
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- Authors
- Huchzermeyer, C., Berndt, N., Holzhütter, H.-G., & Kann, O.
- Affiliations
- Charité – Universitätsmedizin Berlin (DE)
- Heidelberg University (DE)
- Corresponding author
- Holzhütter, Hermann-Georg
- Venue
- Journal of Cerebral Blood Flow & Metabolism
- Publisher
- SAGE Publications
- Published
- 2012-11-21
- Volume
- 33
- Issue
- 2
- Pages
- 263-271
- ISSN
- 0271-678X (ISSN-L)
- 1559-7016
- PMC
- PMC3564197
- OpenAlex
- W2033642739
- Cited by
- 81
- References
- 46
- Citations by year
- 2026: 5 · 2025: 4 · 2024: 5 · 2023: 4 · 2022: 4 · 2021: 9 · 2020: 11 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 7 · 2015: 10 · 2014: 7 · 2013: 3 · 2012: 1
- FWCI
- 3.44
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Keywords
- oxygen consumption, hippocampal CA3, gamma oscillations, local field potentials, brain energy metabolism, capillary oxygenation, brain slice culture
- MeSH terms
- Action Potentials, Animals, Hippocampus, Models, Neurological, Nerve Net, Oxygen Consumption, Rats, Wistar, Microdissection, Tissue Culture Techniques, Rats
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