The DOI matches its Crossref record; 48 of the 49 references it cites were found in a registry or an index.
Human neutrophils produce interferon gamma upon stimulation by interleukin-12
Open accessAbstract
Interferon gamma (IFNgamma) is a Th1 cytokine mainly produced by T cells, NK cells and macrophages in response to interleukin (IL)-12. As polymorphonuclear neutrophils (PMN) have been shown to produce and to release numerous cytokines, in particular upon IL-12 stimulation, we investigated the ability of highly purified PMN to secrete IFNgamma. We found that PMN contained a small store of IFNgamma, and that this store was rapidly secreted upon stimulation by degranulating agents such as formyl peptides. Moreover, after a few hours of stimulation with appropriate agents, PMN synthesized IFNgamma. The effect of IL-12 was time- and concentration-dependent, and IL-12 combinations with IL-2, IL-15, IL-18 or LPS were highly synergistic. Cycloheximide inhibited IFNgamma release in such optimal conditions, confirming the ability of PMN to synthesize IFNgamma. IFNgamma synthesis was associated with an increase in specific mRNA content, pointing to a transcriptional mechanism. The IFNgamma produced by PMN was biologically active, as demonstrated by its ability to induce TNFalpha synthesis by PMN themselves or to induce IL-10 synthesis by peripheral blood mononuclear cells. These findings reveal a novel pathway of autocrine and paracrine PMN activation. They also identified a new role for IFNgamma, bridging innate and adaptive immune responses.
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“Interferon-γ released by activated neutrophils induces macrophage activation [219].”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [219]
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“Neutrophils release MPO, which is taken up by residential macrophages expressing macrophage mannose receptors (MMR).”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [219]
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“The interaction between MPO and MMRs leads to the release of ROS (reactive oxygen species) and proinflammatory cytokines (IL-6, IL-8, TNF-α, IL-1, GM-CSF) by macrophages.”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [219]
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Metadata
- Authors
- Ethuin, F., Gérard, B., Benna, J. E., Boutten, A., Gougereot-Pocidalo, M.-A., Jacob, L., & Chollet-Martin, S.
- Affiliations
- Inserm (FR)
- Hôpital Bichat-Claude-Bernard (FR)
- Hôpital Saint-Louis (FR)
- Venue
- Laboratory Investigation
- Publisher
- Elsevier BV
- Published
- 2004-10
- Volume
- 84
- Issue
- 10
- Pages
- 1363-1371
- ISSN
- 0023-6837 (ISSN-L)
- OpenAlex
- W2014770545
- Cited by
- 141
- References
- 49
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 6 · 2023: 6 · 2022: 8 · 2021: 6 · 2020: 5 · 2019: 8 · 2018: 5 · 2017: 3 · 2016: 8 · 2015: 4 · 2014: 6 · 2013: 11 · 2012: 11
- FWCI
- 1.80
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Laboratory Investigation (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Keywords
- interferon-gamma, neutrophils, IL-12, cytokine production, degranulation, formyl peptides, cytokine synergy, IL-15, IL-18, lipopolysaccharide, autocrine activation, innate-adaptive immune bridge, TNF-alpha induction, transcriptional regulation
- MeSH terms
- Adjuvants, Immunologic, Cell Separation, Cells, Cultured, Dose-Response Relationship, Drug, Humans, Interferon-gamma, Lipopolysaccharides, Lymphocyte Activation, N-Formylmethionine Leucyl-Phenylalanine, Neutrophils, RNA, Messenger, Blotting, Western, Cell Degranulation, Interleukin-12, Interleukin-15, Reverse Transcriptase Polymerase Chain Reaction
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