The DOI matches its Crossref record; 48 of the 49 references it cites were found in a registry or an index.
Human neutrophils produce interferon gamma upon stimulation by interleukin-12
Open accessAbstract
Interferon gamma (IFNgamma) is a Th1 cytokine mainly produced by T cells, NK cells and macrophages in response to interleukin (IL)-12. As polymorphonuclear neutrophils (PMN) have been shown to produce and to release numerous cytokines, in particular upon IL-12 stimulation, we investigated the ability of highly purified PMN to secrete IFNgamma. We found that PMN contained a small store of IFNgamma, and that this store was rapidly secreted upon stimulation by degranulating agents such as formyl peptides. Moreover, after a few hours of stimulation with appropriate agents, PMN synthesized IFNgamma. The effect of IL-12 was time- and concentration-dependent, and IL-12 combinations with IL-2, IL-15, IL-18 or LPS were highly synergistic. Cycloheximide inhibited IFNgamma release in such optimal conditions, confirming the ability of PMN to synthesize IFNgamma. IFNgamma synthesis was associated with an increase in specific mRNA content, pointing to a transcriptional mechanism. The IFNgamma produced by PMN was biologically active, as demonstrated by its ability to induce TNFalpha synthesis by PMN themselves or to induce IL-10 synthesis by peripheral blood mononuclear cells. These findings reveal a novel pathway of autocrine and paracrine PMN activation. They also identified a new role for IFNgamma, bridging innate and adaptive immune responses.
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“Interferon-γ released by activated neutrophils induces macrophage activation [219].”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [219]
In the citing paper
“Neutrophils release MPO, which is taken up by residential macrophages expressing macrophage mannose receptors (MMR).”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [219]
In the citing paper
“The interaction between MPO and MMRs leads to the release of ROS (reactive oxygen species) and proinflammatory cytokines (IL-6, IL-8, TNF-α, IL-1, GM-CSF) by macrophages.”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [219]
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Cites
Interleukin-12 and the regulation of innate resistance and adaptive immunity
Trinchieri, G. (2003) · Nature reviews. Immunology · doi:10.1038/nri1001 · Cited by 4,007
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Neutrophil-Derived Proteins: Selling Cytokines by the Pound
Cassatella, M. A. (1999) · Advances in immunology · doi:10.1016/s0065-2776(08)60791-9 · Cited by 558
The intriguing role of polymorphonuclear neutrophils in antitumor reactions
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Yoshimoto, T., Okamura, H., Tagawa, Y., Iwakura, Y., & Nakanishi, K. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.8.3948 · Cited by 380
Intracellular Pool of Vascular Endothelial Growth Factor in Human Neutrophils
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Self-citationG-CSF–stimulated Neutrophils Are a Prominent Source of Functional BLyS
Scapini, P., Nardelli, B., Nadali, G., Calzetti, F., Pizzolo, G., Montecucco, C., & Cassatella, M. A. (2003) · The Journal of Experimental Medicine · doi:10.1084/jem.20021343 · Cited by 324
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Interferon‐γ activation of polymorphonuclear neutrophil function
Ellis, T. N., & Beaman, B. L. (2004) · Immunology · doi:10.1111/j.1365-2567.2004.01849.x · Cited by 285
Cited by
Sorted by citations
Neutrophils in the activation and regulation of innate and adaptive immunity
Mantovani, A., Cassatella, M. A., Costantini, C., & Jaillon, S. (2011) · Nature reviews. Immunology · doi:10.1038/nri3024 · Cited by 3,159
Neutrophils and immunity: challenges and opportunities
Nathan, C. (2006) · Nature reviews. Immunology · doi:10.1038/nri1785 · Cited by 2,971
Neutrophil-derived cytokines: facts beyond expression
Tecchio, C., Micheletti, A., & Cassatella, M. A. (2014) · Frontiers in Immunology · doi:10.3389/fimmu.2014.00508 · Cited by 699
Solana, R., Tarazona, R., Gayoso, I., Lesur, O., Dupuis, G., & Fulop, T. (2012) · Seminars in Immunology · doi:10.1016/j.smim.2012.04.008 · Cited by 567
Neutrophils: Cinderella of innate immune system
Kumar, V., & Sharma, A. (2010) · International Immunopharmacology · doi:10.1016/j.intimp.2010.08.012 · Cited by 455
Metadata
- Authors
- Ethuin, F., Gérard, B., Benna, J. E., Boutten, A., Gougereot-Pocidalo, M.-A., Jacob, L., & Chollet-Martin, S.
- Affiliations
- Inserm (FR)
- Hôpital Bichat-Claude-Bernard (FR)
- Hôpital Saint-Louis (FR)
- Venue
- Laboratory Investigation
- Publisher
- Elsevier BV
- Published
- 2004-10
- Volume
- 84
- Issue
- 10
- Pages
- 1363-1371
- ISSN
- 0023-6837 (ISSN-L)
- OpenAlex
- W2014770545
- Cited by
- 141
- References
- 49
- Citations by year
- 2026: 4 · 2025: 5 · 2024: 6 · 2023: 6 · 2022: 8 · 2021: 6 · 2020: 5 · 2019: 8 · 2018: 5 · 2017: 3 · 2016: 8 · 2015: 4 · 2014: 6 · 2013: 11 · 2012: 11
- FWCI
- 1.80
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Laboratory Investigation (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Life Sciences › Immunology and Microbiology › Immunology › interferon and immune responses
- Keywords
- interferon-gamma, neutrophils, IL-12, cytokine production, degranulation, formyl peptides, cytokine synergy, IL-15, IL-18, lipopolysaccharide, autocrine activation, innate-adaptive immune bridge, TNF-alpha induction, transcriptional regulation
- MeSH terms
- Adjuvants, Immunologic, Cell Separation, Cells, Cultured, Dose-Response Relationship, Drug, Humans, Interferon-gamma, Lipopolysaccharides, Lymphocyte Activation, N-Formylmethionine Leucyl-Phenylalanine, Neutrophils, RNA, Messenger, Blotting, Western, Cell Degranulation, Interleukin-12, Interleukin-15, Reverse Transcriptase Polymerase Chain Reaction
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