Production of lentiviral vectors

A new technique for the assay of infectivity of human adenovirus 5 DNA

Graham, F. L., & van der Eb, A. J. (1973) · Virology · doi:10.1016/0042-6822(73)90341-3 · Cited by 9,734 · see citing works

A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

Boussif, O., Lezoualc’h, F., Zanta, M. A., Djavaheri‐Mergny, M., Scherman, D., DEMENEIX, B. A., & Behr, J. (1995) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.92.16.7297 · Cited by 6,256 · see citing works

Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.

Gossen, M., & Bujard, H. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.12.5547 · Cited by 4,943 · see citing works

In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector

Naldini, L., Blömer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M., & Trono, D. (1996) · Science · doi:10.1126/science.272.5259.263 · Cited by 4,903 · see citing works

A Third-Generation Lentivirus Vector with a Conditional Packaging System

Dull, T., Zufferey, R., Kelly, M., Mandel, R. J., Nguyen, M., Trono, D., & Naldini, L. (1998) · Journal of Virology · doi:10.1128/jvi.72.11.8463-8471.1998 · Cited by 3,341 · see citing works

Gene therapy of human severe combined immunodeficiency (SCID)-X1 disease.

Cavazzana-Calvo, M., Hacein-Bey, S., Basile, G. D. S., Gross, F., Yvon, E., Nusbaum, P., Selz, F., Hue, C., Certain, S., Casanova, J., et al. (2000) · Science · doi:10.1126/science.288.5466.669 · Cited by 2,703 · see citing works

Self-Inactivating Lentivirus Vector for Safe and Efficient In Vivo Gene Delivery

Zufferey, R., Dull, T., Mandel, R. J., Bukovsky, A., Quiroz, D., Naldini, L., & Trono, D. (1998) · Journal of Virology · doi:10.1128/jvi.72.12.9873-9880.1998 · Cited by 1,815 · see citing works

Hematopoietic Stem Cell Gene Therapy with a Lentiviral Vector in X-Linked Adrenoleukodystrophy

Cartier, N., Hacein-Bey-Abina, S., Bartholomae, C. C., Veres, G., Schmidt, M., Kutschera, I., Vidaud, M., Abel, U., Dal-Cortivo, L., Caccavelli, L., et al. (2009) · Science · doi:10.1126/science.1171242 · Cited by 1,508 · see citing works

Transfusion independence and HMGA2 activation after gene therapy of human β-thalassaemia

Cavazzana-Calvo, M., Payen, E., Negre, O., Wang, G., Hehir, K., Fusil, F., Down, J., Denaro, M., Brady, T., Westerman, K., et al. (2010) · Nature · doi:10.1038/nature09328 · Cited by 1,276 · see citing works

Development of a self-inactivating lentivirus vector

Miyoshi, H., Blömer, U., Takahashi, M., Gage, F. H., & Verma, I. M. (1998) · Journal of Virology · doi:10.1128/jvi.72.10.8150-8157.1998 · Cited by 1,196 · see citing works

Lentiviral Hematopoietic Stem Cell Gene Therapy Benefits Metachromatic Leukodystrophy

Biffi, A., Montini, E., Lorioli, L., Cesani, M., Fumagalli, F., Plati, T., Baldoli, C., Martino, S., Calabria, A., Canale, S., et al. (2013) · Science · doi:10.1126/science.1233158 · Cited by 1,180 · see citing works

High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells

Durocher, Y. (2002) · Nucleic Acids Research · doi:10.1093/nar/30.2.e9 · Cited by 1,041 · see citing works

Lentiviral Hematopoietic Stem Cell Gene Therapy in Patients with Wiskott-Aldrich Syndrome

Aiuti, A., Biasco, L., Scaramuzza, S., Ferrua, F., Cicalese, M. P., Baricordi, C., Dionisio, F., Calabria, A., Giannelli, S., Castiello, M. C., et al. (2013) · Science · doi:10.1126/science.1233151 · Cited by 1,025 · see citing works

Production and purification of lentiviral vectors

Tiscórnia, G., Singer, O., & Verma, I. M. (2006) · Nature Protocols · doi:10.1038/nprot.2006.37 · Cited by 974 · see citing works

Production, concentration and titration of pseudotyped HIV-1-based lentiviral vectors

Kutner, R. H., Zhang, X., & Reiser, J. (2009) · Nature Protocols · doi:10.1038/nprot.2009.22 · Cited by 670 · see citing works

The genotoxic potential of retroviral vectors is strongly modulated by vector design and integration site selection in a mouse model of HSC gene therapy

Montini, E., Cesana, D., Schmidt, M., Sanvito, F., Bartholomae, C. C., Ranzani, M., Benedicenti, F., Sergi, L. S., Ambrosi, A., Ponzoni, M., et al. (2009) · Journal of Clinical Investigation · doi:10.1172/jci37630 · Cited by 571 · see citing works

Synthesis, Assembly, and Processing of Viral Proteins

Swanstrom, R. I., & Wills, J. (1997) · Europe PMC (PubMed Central) · Cited by 513 · see citing works

Gene transfer in humans using a conditionally replicating lentiviral vector

Levine, B. L., Humeau, L. M. P. F., Boyer, J., MacGregor, R.-R., Rebello, T., Lü, X., Binder, G. K., Slepushkin, V., Lemiale, F., Mascola, J. R., et al. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0608138103 · Cited by 505 · see citing works

Endogenous microRNA regulation suppresses transgene expression in hematopoietic lineages and enables stable gene transfer

Brown, B. D., Venneri, M. A., Zingale, A., Sergi, L. S., & Naldini, L. (2006) · Nature Medicine · doi:10.1038/nm1398 · Cited by 484 · see citing works

Long-term safety and tolerability of ProSavin, a lentiviral vector-based gene therapy for Parkinson's disease: a dose escalation, open-label, phase 1/2 trial

Palfi, S., Gurruchaga, J. M., Ralph, G. S., Lepetit, H., Lavisse, S., Buttery, P. C., Watts, C., Miskin, J., Kelleher, M., Deeley, S., et al. (2014) · The Lancet · doi:10.1016/s0140-6736(13)61939-x · Cited by 452 · see citing works

Optimized large-scale production of high titer lentivirus vector pseudotypes

Sena‐Esteves, M., Tebbets, J. C., Steffens, S., Crombleholme, T. M., & Flake, A. W. (2004) · Journal of Virological Methods · doi:10.1016/j.jviromet.2004.08.017 · Cited by 287 · see citing works

Stable gene transfer to the nervous system using a non-primate lentiviral vector

Mitrophanous, K. A., Yoon, S., Rohll, J. B., Patil, D., Wilkes, F. J., Kim, V. N., Kingsman, S. M., Kingsman, A. J., & Mazarakis, N. D. (1999) · Gene Therapy · doi:10.1038/sj.gt.3301023 · Cited by 277 · see citing works

A Packaging Cell Line for Lentivirus Vectors

Kafri, T., van Praag, H., Ouyang, L., Gage, F. H., & Verma, I. (1999) · Journal of Virology · doi:10.1128/jvi.73.1.576-584.1999 · Cited by 276 · see citing works

Minimal Requirement for a Lentivirus Vector Based on Human Immunodeficiency Virus Type 1

Kim, V. N., Mitrophanous, K. A., Kingsman, S. M., & Kingsman, A. J. (1998) · Journal of Virology · doi:10.1128/jvi.72.1.811-816.1998 · Cited by 269 · see citing works

Large-Scale transfection of mammalian cells for the fast production of recombinant protein

Pham, P. L., Kamen, A., & Durocher, Y. (2006) · Molecular Biotechnology · doi:10.1385/mb:34:2:225 · Cited by 236 · see citing works

A Rev-Independent Human Immunodeficiency Virus Type 1 (HIV-1)-Based Vector That Exploits a Codon-Optimized HIV-1 gag-pol Gene

Kotsopoulou, E., Kim, V. N., Kingsman, A. J., Kingsman, S. M., & Mitrophanous, K. A. (2000) · Journal of Virology · doi:10.1128/jvi.74.10.4839-4852.2000 · Cited by 228 · see citing works

A Stable System for the High-Titer Production of Multiply Attenuated Lentiviral Vectors

Klages, N., Zufferey, R., & Trono, D. (2000) · Molecular Therapy · doi:10.1006/mthe.2000.0103 · Cited by 219 · see citing works

Continuous high-titer HIV-1 vector production

Ikeda, Y., Takeuchi, Y., Martı́n, F., Cosset, F., Mitrophanous, K. A., & Collins, M. K. (2003) · Nature Biotechnology · doi:10.1038/nbt815 · Cited by 198 · see citing works

Mystery solved: VSV-G-LVs do not allow efficient gene transfer into unstimulated T cells, B cells, and HSCs because they lack the LDL receptor

Amirache, F., Lévy, C., Costa, C., Mangeot, P.-E., Torbett, B. E., Wang, C. X., Nègre, D., Cosset, F.-L., & Verhoeyen, E. (2014) · Blood · doi:10.1182/blood-2013-11-540641 · Cited by 197 · see citing works

Large-Scale Manufacture and Characterization of a Lentiviral Vector Produced for Clinical Ex Vivo Gene Therapy Application

Merten, O.-W., Charrier, S., Laroudie, N., Fauchille, S., Dugué, C., Jenny, C., Audit, M., Zanta, M.-A., Chautard, H., Radrizzani, M., et al. (2010) · Human Gene Therapy · doi:10.1089/hum.2010.060 · Cited by 186 · see citing works

Continuous propagation of RRE(-) and Rev(-)RRE(-) human immunodeficiency virus type 1 molecular clones containing a cis-acting element of simian retrovirus type 1 in human peripheral blood lymphocytes

Zolotukhin, A. S., Valentin, A., Pavlakis, G. N., & Felber, B. K. (1994) · Journal of Virology · doi:10.1128/jvi.68.12.7944-7952.1994 · Cited by 176 · see citing works

Production and concentration of pseudotyped HIV-1-based gene transfer vectors

Reiser, J. (2000) · Gene Therapy · doi:10.1038/sj.gt.3301188 · Cited by 176 · see citing works

Generation of a Stable Cell Line Producing High-Titer Self-Inactivating Lentiviral Vectors

Xu, K., Ma, H., McCown, T. J., Verma, I. M., & Kafri, T. (2001) · Molecular Therapy · doi:10.1006/mthe.2000.0238 · Cited by 168 · see citing works

A New-Generation Stable Inducible Packaging Cell Line for Lentiviral Vectors

Farson, D., Witt, R., McGuinness, R., Dull, T., Kelly, M. C., Song, J.-P., Radeke, R., Bukovsky, A., Consiglio, A., & Naldini, L. (2001) · Human Gene Therapy · doi:10.1089/104303401750195935 · Cited by 166 · see citing works

Baboon envelope pseudotyped LVs outperform VSV-G-LVs for gene transfer into early-cytokine-stimulated and resting HSCs.

Girard-Gagnepain, A., Amirache, F., Costa, C., Lévy, C., Frecha, C., Fusil, F., Nègre, D., Lavillette, D., Cosset, F., & Verhoeyen, E. (2014) · Blood · doi:10.1182/blood-2014-02-558163 · Cited by 160 · see citing works

Liver-directed lentiviral gene therapy in a dog model of hemophilia B

Cantore, A., Ranzani, M., Bartholomae, C. C., Volpin, M., Valle, P. D., Sanvito, F., Sergi, L. S., Gallina, P., Benedicenti, F., Bellinger, D., et al. (2015) · Science Translational Medicine · doi:10.1126/scitranslmed.aaa1405 · Cited by 158 · see citing works

Upscaling of lentiviral vector production by tangential flow filtration

Geraerts, M., Michiels, M., Baekelandt, V., Debyser, Z., & Gijsbers, R. (2005) · The Journal of Gene Medicine · doi:10.1002/jgm.778 · Cited by 158 · see citing works

Optimized lentiviral vector production and purification procedure prevents immune response after transduction of mouse brain

Baekelandt, V, Eggermont, K., Michiels, M., Nuttin, B., & Debyser, Z. (2003) · Gene Therapy · doi:10.1038/sj.gt.3302094 · Cited by 153 · see citing works

Efficient construction of producer cell lines for a SIN lentiviral vector for SCID-X1 gene therapy by concatemeric array transfection

Throm, R. E., Ouma, A. A., Zhou, S., Chandrasekaran, A., Lockey, T. D., Greene, M. R., Ravin, S. S. D., Moayeri, M., Malech, H. L., Sorrentino, B. P., et al. (2009) · Blood · doi:10.1182/blood-2008-11-191049 · Cited by 149 · see citing works

HIV-Based Vectors: Preparation and Use

Follenzi, A., & Naldini, L. (2003) · Humana Press eBooks · doi:10.1385/1-59259-141-8:259 · Cited by 144 · see citing works

Development of a scalable process for high‐yield lentiviral vector production by transient transfection of HEK293 suspension cultures

Ansorge, S., Lanthier, S., Transfiguracion, J. C., Durocher, Y., Henry, O., & Kamen, A. (2009) · The Journal of Gene Medicine · doi:10.1002/jgm.1370 · Cited by 144 · see citing works

Gene therapy for PIDs: Progress, pitfalls and prospects

Mukherjee, S., & Thrasher, A. J. (2013) · Gene · doi:10.1016/j.gene.2013.03.098 · Cited by 143 · see citing works

Rev-Independent Expression of Synthetic gag-pol Genes of Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus: Implications for the Safety of Lentiviral Vectors

Wagner, R., Graf, M., Bieler, K., Wolf, H. J., Grunwald, T., Foley, P., & Überla, K. (2000) · Human Gene Therapy · doi:10.1089/104303400750038507 · Cited by 138 · see citing works

Inducible Packaging Cells for Large-scale Production of Lentiviral Vectors in Serum-free Suspension Culture

Broussau, S., Jabbour, N., Lachapelle, G., Durocher, Y., Tom, R. L., Transfiguracion, J. C., Gilbert, R., & Bernard Massie, B. (2008) · Molecular Therapy · doi:10.1038/sj.mt.6300383 · Cited by 135 · see citing works

Lentiviral vectors: optimization of packaging, transduction and gene expression

Delenda, C. (2004) · The Journal of Gene Medicine · doi:10.1002/jgm.501 · Cited by 133 · see citing works

Production of lentiviral vectors by large‐scale transient transfection of suspension cultures and affinity chromatography purification

Segura, M. M., Garnier, A., Durocher, Y., Coelho, H., & Kamen, A. (2007) · Biotechnology and Bioengineering · doi:10.1002/bit.21467 · Cited by 130 · see citing works

Requirements for Efficient Production and Transduction of Human Immunodeficiency Virus Type 1-Based Vectors

Gasmi, M., Glynn, J. M., Jin, M., Jolly, D. J., Yee, J., & Chen, S. (1999) · Journal of Virology · doi:10.1128/jvi.73.3.1828-1834.1999 · Cited by 129 · see citing works

Performance-enhancing drugs: design and production of redirected chimeric antigen receptor (CAR) T cells

Levine, B. L. (2015) · Cancer Gene Therapy · doi:10.1038/cgt.2015.5 · Cited by 126 · see citing works

Simplified lentivirus vector production in protein-free media using polyethylenimine-mediated transfection

Kuroda, H., Kutner, R. H., Bazan, N. G., & Reiser, J. (2009) · Journal of Virological Methods · doi:10.1016/j.jviromet.2008.11.021 · Cited by 112 · see citing works

Efficient Transduction of Nondividing Cells by Optimized Feline Immunodeficiency Virus Vectors

Curran, M. A., Kaiser, S. M., Achacoso, P. L., & Nolan, G. P. (2000) · Molecular Therapy · doi:10.1006/mthe.1999.0007 · Cited by 111 · see citing works

Large-Scale Processing of Recombinant Retroviruses for Gene Therapy

Andreadis, S. T., Roth, C. M., Doux, J. M. L., Morgan, J. R., & Yarmush, M. L. (1999) · Biotechnology Progress · doi:10.1021/bp980106m · Cited by 108 · see citing works

Construction of stable packaging cell lines for clinical lentiviral vector production

Sanber, K. S., Knight, S. B., Stephen, S. L., Bailey, R., Escors, D., Minshull, J., Santilli, G., Thrasher, A. J., Collins, M. K., & Takeuchi, Y. (2015) · Scientific Reports · doi:10.1038/srep09021 · Cited by 108 · see citing works

Identification of a Human Immunodeficiency Virus Type 2 (HIV-2) Encapsidation Determinant and Transduction of Nondividing Human Cells by HIV-2-Based Lentivirus Vectors

Poeschla, E. M., Gilbert, J., Li, X., Huang, S., Ho, A. D., & Wong‐Staal, F. (1998) · Journal of Virology · doi:10.1128/jvi.72.8.6527-6536.1998 · Cited by 99 · see citing works

Purification of retroviral vectors for clinical application: Biological implications and technological challenges

Rodrigues, T., Carrondo, M. J. T., Alves, P. M., & Cruz, P. E. (2006) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2006.07.028 · Cited by 98 · see citing works

Safety profile of recombinant adeno-associated viral vectors: focus on alipogene tiparvovec (Glybera ® )

Salmon, F., Grosios, K., & Petry, H. (2013) · Expert Review of Clinical Pharmacology · doi:10.1586/17512433.2014.852065 · Cited by 93 · see citing works

Downstream processing of oncoretroviral and lentiviral gene therapy vectors

Segura, M. M., Kamen, A., & Garnier, A. (2006) · Biotechnology Advances · doi:10.1016/j.biotechadv.2005.12.001 · Cited by 93 · see citing works

Lentivirus Vector Purification Using Anion Exchange HPLC Leads to Improved Gene Transfer

Yamada, K., McCarty, D. M., Madden, V. J., & Walsh, C. (2003) · BioTechniques · doi:10.2144/03345dd04 · Cited by 92 · see citing works

Development of a sensitive assay for detection of replication-competent recombinant lentivirus in large-scale HIV-based vector preparations

Escarpe, P. A., Zayek, N., Chin, P. S., Borellini, F., Zufferey, R., Veres, G., & Kiermer, V. (2003) · Molecular Therapy · doi:10.1016/s1525-0016(03)00167-9 · Cited by 86 · see citing works

Gene transduction efficiency in cells of different species by HIV and EIAV vectors

Ikeda, Y., Collins, M. K., Radcliffe, P. A., Mitrophanous, K., & Takeuchi, Y. (2002) · Gene Therapy · doi:10.1038/sj.gt.3301708 · Cited by 82 · see citing works

Strategies for Targeting Lentiviral Vectors

Frecha, C. A., Szécsi, J., Cosset, F., & Verhoeyen, E. (2008) · Current Gene Therapy · doi:10.2174/156652308786848003 · Cited by 82 · see citing works

The HIV-1 Rev response element

Fernandes, J. D., Jayaraman, B., & Frankel, A. D. (2012) · RNA Biology · doi:10.4161/rna.9.1.18178 · Cited by 81 · see citing works

Production of CGMP-Grade Lentiviral Vectors.

Ausubel, L. J., Hall, C., Sharma, A., Shakeley, R., Lopez, P., Quezada, V., Couture, S., Laderman, K., McMahon, R., Huang, P., et al. (2012) · PubMed · Cited by 79 · see citing works

Cocal-pseudotyped Lentiviral Vectors Resist Inactivation by Human Serum and Efficiently Transduce Primate Hematopoietic Repopulating Cells

Trobridge, G. D., Wu, R. A., Hansen, M. J., Ironside, C. M., Watts, K. L., Olsen, P., Beard, B. C., & Kiem, H. (2009) · Molecular Therapy · doi:10.1038/mt.2009.282 · Cited by 79 · see citing works

Generation of a packaging cell line for prolonged large‐scale production of high‐titer HIV‐1‐based lentiviral vector

Ni, Y., Sun, S. Y., Oparaocha, I., Humeau, L. M. P. F., Davis, B., Cohen, R., Binder, G. K., Chang, Y., Slepushkin, V., & Dropulić, B. (2005) · The Journal of Gene Medicine · doi:10.1002/jgm.726 · Cited by 77 · see citing works

Efficient gene transfer into the CNS by lentiviral vectors purified by anion exchange chromatography

Scherr, M., Battmer, K., Eder, M., Schüle, S., Hohenberg, H., Ganser, A., Grez, M., & Blömer, U. (2002) · Gene Therapy · doi:10.1038/sj.gt.3301848 · Cited by 77 · see citing works

Inducible human immunodeficiency virus type 1 packaging cell lines

Yu, H., Rabson, A. B., Kaul, M., Ron, Y., & Dougherty, J. (1996) · Journal of Virology · doi:10.1128/jvi.70.7.4530-4537.1996 · Cited by 76 · see citing works

An Inducible Packaging Cell System for Safe, Efficient Lentiviral Vector Production in the Absence of HIV-1 Accessory Proteins

Pacchia, A. L., Adelson, M. E., Kaul, M., Ron, Y., & Dougherty, J. P. (2001) · Virology · doi:10.1006/viro.2000.0787 · Cited by 73 · see citing works

Large-scale Purification of a Lentiviral Vector by Size Exclusion Chromatography or Mustang Q Ion Exchange Capsule

Slepushkin, V., Dropulić, B., Chang, N., Gan, Y., Jiang, B., Deausen, E., Berlinger, D., Binder, G. K., Andre, K., Humeau, L. M. P. F., et al. (2003) · BioProcessing Journal · doi:10.12665/j25.dropulic · Cited by 73 · see citing works

Comparison of Transfection Conditions for a Lentivirus Vector Produced in Large Volumes

Karolewski, B. A., Watson, D. J., Parente, M. K., & Wolfe, J. H. N. (2003) · Human Gene Therapy · doi:10.1089/104303403322319372 · Cited by 70 · see citing works

[27] Design, production, safety, evaluation, and clinical applications of nonprimate lentiviral vectors

Rohll, J. B., Mitrophanous, K. A., Martin‐Rendon, E., Ellard, F. M., Radcliffe, P. A., Mazarakis, N. D., & Kingsman, S. M. (2002) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(02)46072-7 · Cited by 68 · see citing works

Safe two‐plasmid production for the first clinical lentivirus vector that achieves >99% transduction in primary cells using a one‐step protocol

Lü, X., Humeau, L. M. P. F., Slepushkin, V., Binder, G. K., Yu, Q., Slepushkina, T., Chen, Z., Merling, R. K., Davis, B., Chang, Y., et al. (2004) · The Journal of Gene Medicine · doi:10.1002/jgm.593 · Cited by 68 · see citing works

A New Generation of Retroviral Producer Cells: Predictable and Stable Virus Production by Flp-Mediated Site-Specific Integration of Retroviral Vectors

Schucht, R., Coroadinha, A. S., Zanta, M.-A., Verhoeyen, E., Carrondo, M. J. T., Häuser, H., & Wirth, D. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2005.12.003 · Cited by 62 · see citing works

Transfection of HEK293-EBNA1 Cells in Suspension with Linear PEI for Production of Recombinant ProteinsPreprint

Tom, R., Bisson, L., & Durocher, Y. (2008) · Cold Spring Harbor Protocols · doi:10.1101/pdb.prot4977 · Cited by 62 · see citing works

The use of recombinase mediated cassette exchange in retroviral vector producer cell lines: Predictability and efficiency by transgene exchange

Coroadinha, A. S., Schucht, R., Gama-Norton, L., Wirth, D., Häuser, H., & Carrondo, M. J. T. (2006) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2006.01.037 · Cited by 61 · see citing works

Certification assays for HIV-1-based vectors: frequent passage of gag sequences without evidence of replication-competent viruses

Sastry, L., Xu, Y., Johnson, T., Desai, K., Rissing, D., Marsh, J. A., & Cornetta, K. G. (2003) · Molecular Therapy · doi:10.1016/j.ymthe.2003.08.003 · Cited by 60 · see citing works

A trans-lentiviral packaging cell line for high-titer conditional self-inactivating HIV-1 vectors

Cockrell, A. S., Ma, H., Fu, K., McCown, T. J., & Kafri, T. (2006) · Molecular Therapy · doi:10.1016/j.ymthe.2005.12.015 · Cited by 58 · see citing works

Strategies in Downstream Processing

Chisti, Y. (1998) · doi:10.1002/9783527619641.ch18 · Cited by 56 · see citing works

Optimisation of a multipartite human immunodeficiency virus based vector system; control of virus infectivity and large-scale production

Koldej, R. M., Cmielewski, P., Stocker, A. G., Parsons, D., & Anson, D. S. (2005) · The Journal of Gene Medicine · doi:10.1002/jgm.803 · Cited by 56 · see citing works

Regulated Lentiviral Packaging Cell Line Devoid of Most Viralcis-Acting Sequences

Kaul, M., Yu, H., Ron, Y., & Dougherty, J. P. (1998) · Virology · doi:10.1006/viro.1998.9327 · Cited by 54 · see citing works

Generation of a Flexible Cell Line with Regulatable, High-Level Expression of HIV Gag/Pol Particles Capable of Packaging HIV-Derived Vectors

Sparacio, S., Pfeiffer, T., Schaal, H., & Bosch, V. (2001) · Molecular Therapy · doi:10.1006/mthe.2001.0296 · Cited by 54 · see citing works

Mobilization and Mechanism of Transcription of Integrated Self-Inactivating Lentiviral Vectors

Hanawa, H., Persons, D. A., & Nienhuis, A. W. (2005) · Journal of Virology · doi:10.1128/jvi.79.13.8410-8421.2005 · Cited by 54 · see citing works

Gene Transfer Systems Derived from Visna Virus: Analysis of Virus Production and Infectivity

Berkowitz, R. D., Ilves, H., Plavec, I., & Veres, G. (2001) · Virology · doi:10.1006/viro.2000.0659 · Cited by 51 · see citing works

Development of inducible EIAV-based lentiviral vector packaging and producer cell lines

Stewart, H. J., Leroux-Carlucci, M. A., Sion, C. J. M., Mitrophanous, K. A., & Radcliffe, P. A. (2009) · Gene Therapy · doi:10.1038/gt.2009.20 · Cited by 51 · see citing works

Small- to Large-Scale Production of Lentivirus Vectors

Marino, M. P., Luce, M. J., & Reiser, J. (2003) · Humana Press eBooks · doi:10.1385/1-59259-393-3:43 · Cited by 48 · see citing works

Transduction of Human CD34 + Repopulating Cells with a Self-Inactivating Lentiviral Vector for SCID-X1 Produced at Clinical Scale by a Stable Cell Line

Greene, M. R., Lockey, T. D., Mehta, P. K., Kim, Y., Eldridge, P. W., Gray, J. T., & Sorrentino, B. P. (2012) · Human Gene Therapy Methods · doi:10.1089/hgtb.2012.150 · Cited by 47 · see citing works

Overview of the HIV‐1 Lentiviral Vector System

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Identification of a Novel Posttranscriptional Regulatory Element by Using a rev- and RRE-Mutated Human Immunodeficiency Virus Type 1 DNA Proviral Clone as a Molecular Trap

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Mesenchymal stem cells as the game‐changing tools in the treatment of various organs disorders: Mirage or reality?

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Integrated development of enzymatic DNA digestion and membrane chromatography processes for the purification of therapeutic adenoviruses

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Cellular Proteo-Transcriptomic Changes in the Immediate Early-Phase of Lentiviral Transduction

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Lentiviral Vector Production from a Stable Packaging Cell Line Using a Packed Bed Bioreactor

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Analytics of host cell proteins (HCPs): lessons from biopharmaceutical mAb analysis for Gene therapy products

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Influence of the isolation method on characteristics and functional activity of mesenchymal stromal cell-derived extracellular vesicles

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Optimized protocol for high‐titer lentivirus production and transduction of primary fibroblasts

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An Update on the HIV DNA Vaccine Strategy

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Possibility of exosome‑based coronavirus disease 2019 vaccine (Review)

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A lentiviral vector for the production of T cells with an inducible transgene and a constitutively expressed tumour-targeting receptor

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Establishment of a simple and efficient platform for car-t cell generation and expansion: from lentiviral production to in vivo studies

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Benchmarking of Scale-X Bioreactor System in Lentiviral and Adenoviral Vector Production

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Production of Lentiviral Vectors Using a HEK-293 Producer Cell Line and Advanced Perfusion Processing

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A Microfluidic Device to Enhance Viral Transduction Efficiency During Manufacture of Engineered Cellular Therapies

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Characterization of Extracellular Vesicles Secreted in Lentiviral Producing HEK293SF Cell Cultures

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Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair

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The transformative potential of HSC gene therapy as a genetic medicine

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Size-Controlled and Shelf-Stable DNA Particles for Production of Lentiviral Vectors

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The FDA’s Regulatory Framework for Chimeric Antigen Receptor‐T Cell Therapies

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Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region

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Cell-based artificial APC resistant to lentiviral transduction for efficient generation of CAR-T cells from various cell sources

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Improved GaLV-TR Glycoproteins to Pseudotype Lentiviral Vectors: Impact of Viral Protease Activity in the Production of LV Pseudotypes

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CRISPR/Cas9-Mediated Genome Editing for Huntington’s Disease

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Preclinical Proof-of-Concept, Analytical Development, and Commercial Scale Production of Lentiviral Vector in Adherent Cells

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Enhancing the purification of Lentiviral vectors for clinical applications

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CURING HEMOGLOBINOPATHIES: CHALLENGES AND ADVANCES OF CONVENTIONAL AND NEW GENE THERAPY APPROACHES.

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Gene therapy process change evaluation framework: Transient transfection and stable producer cell line comparison

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Recent Developments in SARS-CoV-2 Neutralizing Antibody Detection Methods

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Process intensification for lentiviral vector manufacturing using tangential flow depth filtration

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50‐nm Gas‐Filled Protein Nanostructures to Enable the Access of Lymphatic Cells by Ultrasound Technologies

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Multivariate data analysis in cell gene therapy manufacturing

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Update for Advance CAR-T Therapy in Solid Tumors, Clinical Application in Peritoneal Carcinomatosis From Colorectal Cancer and Future Prospects

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A robust and efficient alluvial filtration method for the clarification of adeno-associated viruses from crude cell lysates

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Transient gene expression optimization and expression vector comparison to improve HIV-1 VLP production in HEK293 cell lines

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Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell

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Myocardial mitochondrial antiviral signaling protein promotes heart Ischemia-reperfusion injury via RIG-I signaling in mice

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Development of a perfusion process for continuous lentivirus production using stable suspension producer cell lines

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Delivery of large transgene cassettes by foamy virus vector

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Evaluation of lentiviral vector stability and development of ion exchange purification processes

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Time‐dependent sorption behavior of lentiviral vectors during anion‐exchange chromatography

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Possibilities and efficiency of MSC co-transfection for gene therapy

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Generation of High-Titer Self-Inactivated γ-Retroviral Vector Producer Cells

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A new simplified clarification approach for lentiviral vectors using diatomaceous earth improves throughput and safe handling

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Envelope-Specific Adaptive Immunity following Transplantation of Hematopoietic Stem Cells Modified with VSV-G Lentivirus

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Advances in HIV Gene Therapy

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Current Update on Severe Acute Respiratory Syndrome Coronavirus 2 Vaccine Development with a Special Emphasis on Gene Therapy Viral Vector Design and Construction for Vaccination

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Targeted delivery of genome editors in vivo

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Viral Generation, Packaging, and Transduction on a Digital Microfluidic Platform

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A Scalable Lentiviral Vector Production and Purification Method Using Mustang Q Chromatography and Tangential Flow Filtration

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Correcting inborn errors of immunity: From viral mediated gene addition to gene editing

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Large-Scale Production Means for the Manufacturing of Lentiviral Vectors

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Large-Scale Production of Lentiviral Vectors: Current Perspectives and Challenges

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Recent progress in lentiviral vector mass production

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Upstream insights for lentiviral vector production: cell platforms, culture parameters, and titer yields

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Enabling development of commercial-ready lentiviral vector manufacturing processes using stable producer cell lines

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