Cohesin relocation from sites of chromosomal loading to places of convergent transcription
Open accessAbstract
Sister chromatids, the products of eukaryotic DNA replication, are held together by the chromosomal cohesin complex after their synthesis. This allows the spindle in mitosis to recognize pairs of replication products for segregation into opposite directions. Cohesin forms large protein rings that may bind DNA strands by encircling them, but the characterization of cohesin binding to chromosomes in vivo has remained vague. We have performed high resolution analysis of cohesin association along budding yeast chromosomes III-VI. Cohesin localizes almost exclusively between genes that are transcribed in converging directions. We find that active transcription positions cohesin at these sites, not the underlying DNA sequence. Cohesin is initially loaded onto chromosomes at separate places, marked by the Scc2/Scc4 cohesin loading complex, from where it appears to slide to its more permanent locations. But even after sister chromatid cohesion is established, changes in transcription lead to repositioning of cohesin. Thus the sites of cohesin binding and therefore probably sister chromatid cohesion, a key architectural feature of mitotic chromosomes, display surprising flexibility. Cohesin localization to places of convergent transcription is conserved in fission yeast, suggesting that it is a common feature of eukaryotic chromosomes.
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Cohesin can remain topologically engaged and slide on both naked DNA and chromatin.
“Since cohesin can remain topologically engaged and slide on both naked DNA42–44 and chromatin,45,46”
From Recent evidence that TADs and chromatin loops are dynamic structures (2018) · cites this paper as [45]
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- Authors
- Lengronne, A., Katou, Y., Mori, S., Yokobayashi, S., Kelly, G. P., Itoh, T., Watanabe, Y., Shirahige, K., & Uhlmann, F.
- Affiliations
- Cancer Research UK (GB)
- RIKEN (JP)
- Tokyo Institute of Technology (JP)
- Institute for Quantitative Biosciences, The University of Tokyo (JP)
- Mitsubishi Research Institute (Japan) (JP)
- Japan Science and Technology Agency (JP)
- Corresponding author
- Uhlmann, Frank
- ORCID
- Venue
- Nature
- Published
- 2004-06-30
- Volume
- 430
- Issue
- 6999
- Pages
- 573-578
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC2610358
- OpenAlex
- W1978696228
- Cited by
- 588
- References
- 34
- Citations by year
- 2026: 16 · 2025: 17 · 2024: 25 · 2023: 19 · 2022: 22 · 2021: 33 · 2020: 29 · 2019: 37 · 2018: 25 · 2017: 32 · 2016: 25 · 2015: 17 · 2014: 26 · 2013: 22 · 2012: 20
- FWCI
- 12.34
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Institutional Repositories DataBase (IRDB) (submittedVersion)
- Tokyo Tech Research Repository (Tokyo Institute of Technology) (submittedVersion)
- Funders
- Cancer Research UK (A3592)
- Ministry of Education, Culture, Sports, Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Keywords
- cohesin, sister chromatid cohesion, convergent transcription, metaphase chromosomes, sister chromatid segregation, DNA replication, Saccharomyces cerevisiae, Schizosaccharomyces pombe
- MeSH terms
- Cohesins, Chromatids, Chromosomal Proteins, Non-Histone, Fungal Proteins, Genes, Fungal, Nuclear Proteins, Protein Binding, Saccharomyces cerevisiae, Schizosaccharomyces, Transcription, Genetic, Chromosomes, Fungal, Conserved Sequence, Cell Cycle Proteins, Chromosome Segregation, DNA, Intergenic
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