A histone H3 methyltransferase controls epigenetic events required for meiotic prophase
Abstract
Epigenetic modifications of histones regulate gene expression and chromatin structure. Here we show that Meisetz (meiosis-induced factor containing a PR/SET domain and zinc-finger motif) is a histone methyltransferase that is important for the progression of early meiotic prophase. Meisetz transcripts are detected only in germ cells entering meiotic prophase in female fetal gonads and in postnatal testis. Notably, Meisetz has catalytic activity for trimethylation, but not mono- or dimethylation, of lysine 4 of histone H3, and a transactivation activity that depends on its methylation activity. Mice in which the Meisetz gene is disrupted show sterility in both sexes due to severe impairment of the double-stranded break repair pathway, deficient pairing of homologous chromosomes and impaired sex body formation. In Meisetz-deficient testis, trimethylation of lysine 4 of histone H3 is attenuated and meiotic gene transcription is altered. These findings indicate that meiosis-specific epigenetic events in mammals are crucial for proper meiotic progression.
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Prdm9 knockout mice demonstrate abnormal location of recombination hotspots with enrichment at gene promoters and enhancers, fail to properly repair double-stranded breaks, and are infertile.
“Prdm9 knockout mice demonstrate abnormal location of recombination hotspots with enrichment at gene promoters and enhancers, and also fail to properly repair double-stranded breaks and are infertile (both sexes sterile)(21, 22).”
From Health and population effects of rare gene knockouts in adult humans with related parents (2016) · cites this paper as [21]
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- Authors
- Hayashi, K., Yoshida, K., & Matsui, Y.
- Affiliations
- Osaka Women's and Children's Hospital (JP)
- Osaka City University (JP)
- Tohoku University (JP)
- Corresponding author
- Matsui, Yasuhisa
- Venue
- Nature
- Published
- 2005-11
- Volume
- 438
- Issue
- 7066
- Pages
- 374-378
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2071936353
- Cited by
- 472
- References
- 28
- Citations by year
- 2026: 10 · 2025: 14 · 2024: 10 · 2023: 12 · 2022: 18 · 2021: 24 · 2020: 28 · 2019: 23 · 2018: 28 · 2017: 27 · 2016: 39 · 2015: 26 · 2014: 25 · 2013: 22 · 2012: 43
- FWCI
- 5.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Core Research for Evolutional Science and Technology
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Keywords
- histone methyltransferase, meiotic prophase, meiotic recombination, homologous chromosome pairing, DNA double-strand break repair, spermatocytes, transcriptional activation, germ cells
- MeSH terms
- Histone Methyltransferases, Animals, Cloning, Molecular, Female, Gene Expression Regulation, Gonads, Histones, Male, Meiosis, Methylation, Mice, Inbred C57BL, Prophase, Histone-Lysine N-Methyltransferase, Protein Methyltransferases, Spermatocytes, Transcription, Genetic, Epigenesis, Genetic, Mice
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