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Direct reprogramming of human neural stem cells by OCT4

Kim, Jeong Beom, Greber, Boris, Araúzo-Bravo, Marcos J., Meyer, Johann, Park, Kook In, Zaehres, Holm, Schöler, Hans R.·2009·Nature
DOI 10.1038/nature08436
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Abstract

Induced pluripotent stem (iPS) cells have been generated from mouse and human somatic cells by ectopic expression of four transcription factors (OCT4 (also called POU5F1), SOX2, c-Myc and KLF4). We previously reported that Oct4 alone is sufficient to reprogram directly adult mouse neural stem cells to iPS cells. Here we report the generation of one-factor human iPS cells from human fetal neural stem cells (one-factor (1F) human NiPS cells) by ectopic expression of OCT4 alone. One-factor human NiPS cells resemble human embryonic stem cells in global gene expression profiles, epigenetic status, as well as pluripotency in vitro and in vivo. These findings demonstrate that the transcription factor OCT4 is sufficient to reprogram human neural stem cells to pluripotency. One-factor iPS cell generation will advance the field further towards understanding reprogramming and generating patient-specific pluripotent stem cells.

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Crossref · 10.1038/nature08436

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2 of 2 references verified0 citation errors · 0 not checkable

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  • OCT4 overexpression alone is sufficient to induce pluripotency when other canonical reprogramming factors are endogenously expressed or are in the presence of other chromatin remodeling chemical factors.

    “as OCT4 overexpression alone is sufficient to induce pluripotency when other canonical reprogramming factors are endogenously expressed or are in the presence of other chromatin remodeling chemical factors 32,34,35 .”

    From Mechanisms, pathways and strategies for rejuvenation through epigenetic reprogramming (2023) · cites this paper as [34]

Citations

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Research works printed but not linked: 23

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Metadata

Authors
Kim, J. B., Greber, B., Araúzo-Bravo, M. J., Meyer, J., Park, K. I., Zaehres, H., & Schöler, H. R.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2009-08-28
Volume
461
Issue
7264
Pages
649-653
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/nature08436
Cited by
577
References
2
Language
en
Licence
www.springer.com/tdm (tdm)

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