Cited by 547Protein Structure and DynamicsAdvanced NMR Techniques and ApplicationsDiffusion and Search Dynamics

Protein activity regulation by conformational entropy

Tzeng, Shiou-Ru, Kalodimos, Charalampos G.·2012·Nature

Abstract

How the interplay between protein structure and internal dynamics regulates protein function is poorly understood. Often, ligand binding, post-translational modifications and mutations modify protein activity in a manner that is not possible to rationalize solely on the basis of structural data. It is likely that changes in the internal motions of proteins have a major role in regulating protein activity, but the nature of their contributions remains elusive, especially in quantitative terms. Here we show that changes in conformational entropy can determine whether protein-ligand interactions will occur, even among protein complexes with identical binding interfaces. We have used NMR spectroscopy to determine the changes in structure and internal dynamics that are elicited by the binding of DNA to several variants of the catabolite activator protein (CAP) that differentially populate the inactive and active DNA-binding domain states. We found that the CAP variants have markedly different affinities for DNA, despite the CAP−DNA-binding interfaces being essentially identical in the various complexes. Combined with thermodynamic data, the results show that conformational entropy changes can inhibit the binding of CAP variants that are structurally poised for optimal DNA binding or can stimulate the binding activity of CAP variants that only transiently populate the DNA-binding-domain active state. Collectively, the data show how changes in fast internal dynamics (conformational…

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Crossref · 10.1038/nature11271

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Authors
Tzeng, S.-R., & Kalodimos, C. G.
Affiliations
Rutgers, The State University of New Jersey (US)
Corresponding author
Kalodimos, Charalampos G.
Venue
Nature
Published
2012-07-11
Volume
488
Issue
7410
Pages
236-240
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2009239521
Cited by
545
References
30
Citations by year
2026: 25 · 2025: 27 · 2024: 33 · 2023: 43 · 2022: 26 · 2021: 30 · 2020: 29 · 2019: 42 · 2018: 53 · 2017: 40 · 2016: 42 · 2015: 48 · 2014: 47 · 2013: 51 · 2012: 9
FWCI
19.86
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
National Science Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Diffusion and Search Dynamics
Keywords
CAP, protein-DNA interactions, conformational entropy, protein internal dynamics, protein conformational states, DNA-binding domain, protein structure-function relationship, protein variants, binding affinity, binding thermodynamics
MeSH terms
Binding Sites, Cyclic AMP Receptor Protein, DNA, Models, Molecular, Motion, Protein Binding, Time Factors, Protein Structure, Tertiary, Entropy, Nuclear Magnetic Resonance, Biomolecular

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