The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Arteriolar niches maintain haematopoietic stem cell quiescence
Open accessAbstract
Cell cycle quiescence is a critical feature contributing to haematopoietic stem cell (HSC) maintenance. Although various candidate stromal cells have been identified as potential HSC niches, the spatial localization of quiescent HSCs in the bone marrow remains unclear. Here, using a novel approach that combines whole-mount confocal immunofluorescence imaging techniques and computational modelling to analyse significant three-dimensional associations in the mouse bone marrow among vascular structures, stromal cells and HSCs, we show that quiescent HSCs associate specifically with small arterioles that are preferentially found in endosteal bone marrow. These arterioles are ensheathed exclusively by rare NG2 (also known as CSPG4)+ pericytes, distinct from sinusoid-associated leptin receptor (LEPR)+ cells. Pharmacological or genetic activation of the HSC cell cycle alters the distribution of HSCs from NG2+ periarteriolar niches to LEPR+ perisinusoidal niches. Conditional depletion of NG2+ cells induces HSC cycling and reduces functional long-term repopulating HSCs in the bone marrow. These results thus indicate that arteriolar niches are indispensable for maintaining HSC quiescence.
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Citations
Cites
Hematopoiesis: An Evolving Paradigm for Stem Cell Biology
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IFNα activates dormant haematopoietic stem cells in vivo
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Coexistence of Quiescent and Active Adult Stem Cells in Mammals
Li, L., & Clevers, H. (2010) · Science · doi:10.1126/science.1180794 · Cited by 1,247
Bone-marrow adipocytes as negative regulators of the haematopoietic microenvironment
Naveiras, O., Nardi, V., Wenzel, P. L., Hauschka, P. V., Fahey, F., & Daley, G. Q. (2009) · Nature · doi:10.1038/nature08099 · Cited by 1,129 · cited 1×
“Unlike long bones which are mostly occupied by adipocytes in adult humans 29 , the sternum exhibits rich hematopoietic activity in both species.”
Quiescent haematopoietic stem cells are activated by IFN-γ in response to chronic infection
Baldridge, M. T., King, K. Y., Boles, N. C., Weksberg, D. C., & Goodell, M. A. (2010) · Nature · doi:10.1038/nature09135 · Cited by 877
Deconstructing stem cell self-renewal: genetic insights into cell-cycle regulation
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Age-dependent fate and lineage restriction of single NG2 cells
Zhu, X., Hill, R. A., Dietrich, D., Komitova, M., Suzuki, R., & Nishiyama, A. (2011) · Development · doi:10.1242/dev.047951 · Cited by 464
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“The association and quiescence of both haematopoietic and mesenchymal stem cells at this site may thus endow arterioles with the potential for orchestrating haematopoietic and stromal regeneration 40 .”
Self-citation
Cited by
The bone marrow niche for haematopoietic stem cells
Morrison, S. J., & Scadden, D. T. (2014) · Nature · doi:10.1038/nature12984 · Cited by 2,451
Zhou, B. O., Yue, R., Murphy, M. M., Peyer, J. G., & Morrison, S. J. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.06.008 · Cited by 1,381
“Furthermore, Nestin-GFP cells are heterogeneous, including both Nestin-GFP cells that localize mainly around arterioles and Nestin-GFP cells that localize mainly around sinusoids, raising questions about the distribution of HSC niche factors and MSCs among these cell populations (Kunisaki et al., 2013).”
InfluentialImmunoregulatory mechanisms of mesenchymal stem and stromal cells in inflammatory diseases
Shi, Y., Wang, Y., Li, Q., Liu, K., Hou, J., Shao, C., & Wang, Y. (2018) · Nature Reviews Nephrology · doi:10.1038/s41581-018-0023-5 · Cited by 1,223
Direct measurement of local oxygen concentration in the bone marrow of live animals
Spencer, J. A., Ferraro, F., Roussakis, E., Klein, A., Wu, J., Runnels, J. M., Zaher, W., Mortensen, L. J., Alt, C., Turcotte, R., et al. (2014) · Nature · doi:10.1038/nature13034 · Cited by 1,167
Baccin, C., Al-Sabah, J., Velten, L., Helbling, P. M., Grünschläger, F., Hernández-Malmierca, P., Nombela-Arrieta, C., Steinmetz, L. M., Trumpp, A., & Haas, S. (2020) · Nature Cell Biology · doi:10.1038/s41556-019-0439-6 · Cited by 904
Metadata
- Authors
- Kunisaki, Y., Bruns, I., Scheiermann, C., Ahmed, J., Pinho, S., Zhang, D., Mizoguchi, T., Wei, Q., Lucas, D., Ito, K., Mar, J. C., Bergman, A., & Frenette, P. S.
- Venue
- Nature
- Published
- 2013-10-09
- Volume
- 502
- Issue
- 7473
- Pages
- 637-643
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2060930865
- Cited by
- 1,231
- References
- 9
- FWCI
- 59.08
- Type
- journal article
- Language
- en
- Open access
- green
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