The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds
Open accessAbstract
We present a yeast chemical-genomics approach designed to identify genes that when mutated confer drug resistance, thereby providing insight about the modes of action of compounds. We developed a molecular barcoded yeast open reading frame (MoBY-ORF) library in which each gene, controlled by its native promoter and terminator, is cloned into a centromere-based vector along with two unique oligonucleotide barcodes. The MoBY-ORF resource has numerous genetic and chemical-genetic applications, but here we focus on cloning wild-type versions of mutant drug-resistance genes using a complementation strategy and on simultaneously assaying the fitness of all transformants with barcode microarrays. The complementation cloning was validated by mutation detection using whole-genome yeast tiling microarrays, which identified unique polymorphisms associated with a drug-resistant mutant. We used the MoBY-ORF library to identify the genetic basis of several drug-resistant mutants and in this analysis discovered a new class of sterol-binding compounds.
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Self-citationSystematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants
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Self-citationSchuldiner, M., Collins, S. R., Thompson, N. J., Denic, V., Bhamidipati, A., Punna, T., Ihmels, J., Andrews, B., Boone, C., Greenblatt, J. F., et al. (2005) · Cell · doi:10.1016/j.cell.2005.08.031 · Cited by 870 · cited 1×
“Similarly, a strain carrying a different MVD1 hypomorphic allele—an mvd1-DAmP mutant expressing less wild-type protein owing to a deletion of the 3′ untranslated region of MVD1 that reduces mRNA tenfol”
BackgroundSelf-citationCharacteristic genome rearrangements in experimental evolution of Saccharomyces cerevisiae
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Self-citationGresham, D., Desai, M. M., Tucker, C. M., Jenq, H. T., Pai, D. A., Ward, A., DeSevo, C. G., Botstein, D., & Dunham, M. J. (2008) · PLoS Genetics · doi:10.1371/journal.pgen.1000303 · Cited by 482
Self-citationRAPT1, a mammalian homolog of yeast Tor, interacts with the FKBP12/rapamycin complex.
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What would you do if you could sequence everything?
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Natural Products for Drug Discovery in the 21st Century: Innovations for Novel Drug Discovery
Thomford, N. E., Senthebane, D. A., Rowe, A., Munro, D., Seele, P., Maroyi, A., & Dzobo, K. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19061578 · Cited by 1,241
Metabolic engineering of Escherichia coli using synthetic small regulatory RNAs
Na, D., Yoo, S. M., Chung, H., Park, H., Park, J. H., & Lee, S. Y. (2013) · Nature Biotechnology · doi:10.1038/nbt.2461 · Cited by 621
Engineering microbial biofuel tolerance and export using efflux pumps
Dunlop, M. J., Dossani, Z. Y., Szmidt, H. L., Chu, H. C., Lee, T. S., Keasling, J. D., Hadi, M. Z., & Mukhopadhyay, A. (2011) · Molecular Systems Biology · doi:10.1038/msb.2011.21 · Cited by 512
Chromosomal duplication is a transient evolutionary solution to stress
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Quantitative phenotyping via deep barcode sequencing
Smith, A. M., Heisler, L. E., Mellor, J., Kaper, F., Thompson, M. J., Chee, M., Roth, F. P., Giaever, G., & Nislow, C. (2009) · Genome Research · doi:10.1101/gr.093955.109 · Cited by 298
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Metadata
- Authors
- Ho, C. H., Magtanong, L., Barker, S. L., Gresham, D., Nishimura, S., Natarajan, P., Koh, J. L. Y., Porter, J., Gray, C. A., Andersen, R. J., Giaever, G., Nislow, C., Andrews, B., Botstein, D., Graham, T. R., Yoshida, M., & Boone, C.
- Venue
- Nature Biotechnology
- Published
- 2009-04
- Volume
- 27
- Issue
- 4
- Pages
- 369-377
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2108098152
- Cited by
- 292
- References
- 7
- FWCI
- 10.56
- Type
- journal article
- Language
- en
- Open access
- green
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- Ho, C. H., Magtanong, L., Barker, S. L., Gresham, D., Nishimura, S., Natarajan, P., Koh, J. L. Y., Porter, J., Gray, C. A., Andersen, R. J., Giaever, G., Nislow, C., Andrews, B., Botstein, D., Graham, T. R., Yoshida, M., & Boone, C. (2009). A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds. Nature Biotechnology. https://doi.org/10.1038/nbt.1534
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