The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.
Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy
Open accessAbstract
During embryonic development, skeletal muscles arise from somites, which derive from the presomitic mesoderm (PSM). Using PSM development as a guide, we establish conditions for the differentiation of monolayer cultures of mouse embryonic stem (ES) cells into PSM-like cells without the introduction of transgenes or cell sorting. We show that primary and secondary skeletal myogenesis can be recapitulated in vitro from the PSM-like cells, providing an efficient, serum-free protocol for the generation of striated, contractile fibers from mouse and human pluripotent cells. The mouse ES cells also differentiate into Pax7+ cells with satellite cell characteristics, including the ability to form dystrophin+ fibers when grafted into muscles of dystrophin-deficient mdx mice, a model of Duchenne muscular dystrophy (DMD). Fibers derived from ES cells of mdx mice exhibit an abnormal branched phenotype resembling that described in vivo, thus providing an attractive model to study the origin of the pathological defects associated with DMD.
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Self-Organizing 3D Human Trunk Neuromuscular Organoids
Faustino Martins, J.-M., Fischer, C., Urzi, A., Vidal, R., Kunz, S., Ruffault, P.-L., Kabuss, L., Hube, I., Gazzerro, E., Birchmeier, C., et al. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2019.12.007 · Cited by 336
Engineering human pluripotent stem cells into a functional skeletal muscle tissue
Rao, L., Qian, Y., Khodabukus, A., Ribar, T., & Bursac, N. (2018) · Nature Communications · doi:10.1038/s41467-017-02636-4 · Cited by 334
Tissue Stem Cells: Architects of Their Niches
Fuchs, E., & Blau, H. M. (2020) · Cell Stem Cell · doi:10.1016/j.stem.2020.09.011 · Cited by 332
Chal, J., Al Tanoury, Z., Hestin, M., Gobert, B., Aivio, S., Hick, A., Cherrier, T., Nesmith, A. P., Parker, K. K., & Pourquié, O. (2016) · Nature Protocols · doi:10.1038/nprot.2016.110 · Cited by 299
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Metadata
- Authors
- Chal, J., Oginuma, M., Al Tanoury, Z., Gobert, B., Sumara, O., Hick, A., Bousson, F., Zidouni, Y., Mursch, C., Moncuquet, P., Tassy, O., Vincent, S., Miyanari, A., Bera, A., Garnier, J.-M., Guevara, G., Hestin, M., Kennedy, L., Hayashi, S., Drayton, B., Cherrier, T., Gayraud-Morel, B., Gussoni, E., Relaix, F., Tajbakhsh, S., & Pourquié, O.
- Venue
- Nature Biotechnology
- Published
- 2015-09
- Volume
- 33
- Issue
- 9
- Pages
- 962-969
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1953958400
- Cited by
- 427
- References
- 65
- FWCI
- 14.02
- Type
- journal article
- Language
- en
- Open access
- green
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- Chal, J., Oginuma, M., Al Tanoury, Z., Gobert, B., Sumara, O., Hick, A., Bousson, F., Zidouni, Y., Mursch, C., Moncuquet, P., Tassy, O., Vincent, S., Miyanari, A., Bera, A., Garnier, J.-M., Guevara, G., Hestin, M., Kennedy, L., Hayashi, S., . . . Pourquié, O. (2015). Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy. Nature Biotechnology. https://doi.org/10.1038/nbt.3297
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