The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown
Open accessAbstract
A central hurdle in developing small interfering RNAs (siRNAs) as therapeutics is the inefficiency of their delivery across the plasma and endosomal membranes to the cytosol, where they interact with the RNA interference machinery. With the aim of improving endosomal release, a poorly understood and inefficient process, we studied the uptake and cytosolic release of siRNAs, formulated in lipoplexes or lipid nanoparticles, by live-cell imaging and correlated it with knockdown of a target GFP reporter. siRNA release occurred invariably from maturing endosomes within ~5-15 min of endocytosis. Cytosolic galectins immediately recognized the damaged endosome and targeted it for autophagy. However, inhibiting autophagy did not enhance cytosolic siRNA release. Gene knockdown occurred within a few hours of release and required <2,000 copies of cytosolic siRNAs. The ability to detect cytosolic release of siRNAs and understand how it is regulated will facilitate the development of rational strategies for improving the cytosolic delivery of candidate drugs.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source says what the claim attributes to it.
In the citing paper
“∼2000 cytoplasmic siRNAs are estimated to achieve maximal silencing, whereas ∼50 000 are required for an ASO [54, 55].”
From Elucidating brain transport pathways and cell type-dependent gene silencing of a durable lipid–siRNA conjugate administered into cerebrospinal fluid (2025) · cites this paper as [54]
In the citing paper
“Indeed, only a small amount of lipid nanoparticles may be able to escape from the endosome137–139.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [139]
In the citing paper
“Following cellular internalization, lipid nanoparticles are usually trapped in endosomal compartments137–139.”
From Lipid nanoparticles for mRNA delivery (2021) · cites this paper as [139]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
Felgner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan, H. W., Wenz, M., Northrop, J. P., RINGOLD, G. M., & Danielsen, M. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7413 · Cited by 4,954
Huotari, J., & Helenius, A. (2011) · The EMBO Journal · doi:10.1038/emboj.2011.286 · Cited by 2,448
Kimura, S., Noda, T., & Yoshimori, T. (2007) · Autophagy · doi:10.4161/auto.4451 · Cited by 2,278
Rational design of cationic lipids for siRNA delivery
Semple, S. C., Akinc, A., Chen, J., Sandhu, A. P., Mui, B. L., Cho, C. K., Sah, D. W. Y., Stebbing, D., Crosley, E. J., Yaworski, E., et al. (2010) · Nature Biotechnology · doi:10.1038/nbt.1602 · Cited by 1,822
Self-citationDelivery materials for siRNA therapeutics
Kanasty, R., Dorkin, J. R., Vegas, A., & Anderson, D. (2013) · Nature Materials · doi:10.1038/nmat3765 · Cited by 1,800
Gilleron, J., Querbes, W., Zeigerer, A., Borodovsky, A., Marsico, G., Schubert, U., Manygoats, K., Seifert, S., Andree, C., Stöter, M., et al. (2013) · Nature Biotechnology · doi:10.1038/nbt.2612 · Cited by 1,681
Lentivirus-delivered stable gene silencing by RNAi in primary cells.
Stewart, S. A., Dykxhoorn, D. M., Palliser, D., Mizuno, H., Yu, E. Y., An, D., Sabatini, D., Chen, I. S. Y., Hahn, W., Sharp, P., et al. (2003) · RNA: A publication of the RNA Society · doi:10.1261/rna.2192803 · Cited by 1,619
RNAi-mediated gene silencing in non-human primates
Zimmermann, T. S., Lee, A. C. H., Akinc, A., Bramlage, B., Bumcrot, D., Fedoruk, M. N., Harborth, J., Heyes, J. A., Jeffs, L. B., John, M., et al. (2006) · Nature · doi:10.1038/nature04688 · Cited by 1,357
Self-citationMaximizing the potency of siRNA lipid nanoparticles for hepatic gene silencing in vivo
Jayaraman, M., Ansell, S. M., Mui, B. L., Tam, Y. K., Chen, J., Du, X., Butler, D., Eltepu, L., Matsuda, S., Narayanannair, J. K., et al. (2012) · Angewandte Chemie International Edition · doi:10.1002/anie.201203263 · Cited by 1,267
Self-citationTargeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms
Akinc, A., Querbes, W., De, S., Qin, J., Frank-Kamenetsky, M., Jayaprakash, K. N., Jayaraman, M., Rajeev, K. G., Cantley, W. L., Dorkin, J. R., et al. (2010) · Molecular Therapy · doi:10.1038/mt.2010.85 · Cited by 1,261
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Items 401–500 of 690
Deng, S., Shao, H., Shang, H., Pang, L., Chen, X., Cao, J., Wang, Y., & Zhao, Z. (2024) · Vaccines · doi:10.3390/vaccines13010025 · Cited by 14
Unleashing the power of nucleic acid therapeutics through efficient cytosolic delivery
Cao, T. G. N., & Meng, F. (2025) · Journal of Controlled Release · doi:10.1016/j.jconrel.2025.113774 · Cited by 14
Lentiviral Vectors: From Wild-Type Viruses to Efficient Multi-Functional Delivery Vectors
Arrasate, A., Lopez-Robles, C., Zuazo, M., Baños-Mateos, S., Martín, C., Lamsfus‐Calle, A., & Fertin, M. J. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26178497 · Cited by 14
Wonder, E. A., Ewert, K. K., Liu, C., Steffes, V. M., Kwak, J., Qahar, V., Majzoub, R. N., Zhang, Z., Carragher, B. O., Potter, C. S., et al. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.0c10972 · Cited by 14
Genetic analysis of North American Phragmites australis guides management approaches
Lindsay, D. L., Freeland, J. R., Gong, P., Guan, X., Harms, N. E., Kowalski, K. P., Lance, R. F., Oh, D., Sartain, B., & Wendell, D. L. (2022) · Aquatic Botany · doi:10.1016/j.aquabot.2022.103589 · Cited by 14
Translation of in vitro-transcribed RNA therapeutics
von der Haar, T., Mulroney, T. E., Hedayioglu, F. D. L., Kurusamy, S., Rust, M., Lilley, K. S., Thaventhiran, J. E. D., Willis, A. E., & Smales, C. M. (2023) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2023.1128067 · Cited by 14
Polyresorcinols for intracellular protein delivery
Li, Y., Yu, X., Chen, Q., Wang, H., Lv, J., & Cheng, Y. (2024) · Nano Today · doi:10.1016/j.nantod.2024.102287 · Cited by 14
Blitek, M., Phongsavanh, X., & Goyenvalle, A. (2024) · RSC Medicinal Chemistry · doi:10.1039/d4md00394b · Cited by 14
Guo, Z., Gao, S., Xu, F., Chen, Z., Wang, Q., Liu, Z., Wang, Z., Qin, W., Zeng, C., Liu, Z., et al. (2025) · Journal of Nanobiotechnology · doi:10.1186/s12951-025-03108-4 · Cited by 14
Ionizable Lipids with Branched Linkers Enhance the Delivery of mRNA Vaccines
Liu, J., Sun, W., Xiao, B., Xu, H., Fan, J., Shi, X., Pan, Y., Qi, W. D., Li, R., Wang, H., et al. (2025) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.4c21289 · Cited by 14
Cheng, M., Jiao, Z., Lei, J., Li, M., Yang, K., Qi, S., Yu, X., Wang, Y., Yan, L., & Yu, G. (2025) · Journal of the American Chemical Society · doi:10.1021/jacs.5c03033 · Cited by 14
Tanaka, H., Sato, Y., Harashima, H., & Akita, H. (2016) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2016.1154531 · Cited by 15
Addison, M., Ranasinghe, P. D., & Webb, D. J. (2023) · Expert Review of Clinical Pharmacology · doi:10.1080/17512433.2023.2277330 · Cited by 14
Dharshini, K., Mehnath, S., Sathishkumar, G., Ambigaibalan, A. C., & Jeyaraj, M. (2025) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2025.106810 · Cited by 13
Takechi‐Haraya, Y. (2025) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.b25-00158 · Cited by 13
Xu, X., Cui, L., Zhang, Y., & Gu, J. (2026) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2025.11.023 · Cited by 13
Caballero, G. G., Schmidt, S., Schnölzer, M., Schlötzer-Schrehardt, U. M., Knospe, C., Ludwig, A., Manning, J. C., Muschler, P., Kaltner, H., Kopitz, J., et al. (2018) · Cell and Tissue Research · doi:10.1007/s00441-018-2931-x · Cited by 13
Surface Design Options in Polymer- and Lipid-Based siRNA Nanoparticles Using Antibodies
Gabel, M., Knauss, A., Fischer, D., Neurath, M. F., & Weigmann, B. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232213929 · Cited by 13
Repositioning the antihistamine ebastine as an intracellular siRNA delivery enhancer
Muntean, C., Blondeel, E., Harinck, L., Pednekar, K. P., Prakash, J., Wever, O. D., Chain, J. L., Smedt, S. C. D., Remaut, K., & Raemdonck, K. (2023) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2023.123348 · Cited by 13
Wang, S., Guo, B., Wang, H., & Yang, F. (2024) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2024.105547 · Cited by 14
A review of the complex interplay between chemoresistance and lncRNAs in lung cancer
Alnefaie, G. (2024) · Journal of Translational Medicine · doi:10.1186/s12967-024-05877-2 · Cited by 13
Chen, Y., Lombaerde, E. D., Bugler-Lamb, A. R., Zhong, Z., Schuijs, M. J., Gomez, C. M. B., Baere, J. D., Gontsarik, M., Lauwers, H., Deswarte, K., et al. (2025) · Angewandte Chemie International Edition · doi:10.1002/anie.202506954 · Cited by 13
Dissolving Microneedles for Nucleic Acid Delivery: A Systematic Search, Review, and Data Synthesis
Tobos, C. I., & Woodrow, K. A. (2024) · Acta Biomaterialia · doi:10.1016/j.actbio.2025.05.025 · Cited by 13
Precise Enzymatic Cleavage Sites for Improved Bioactivity of siRNA Lipo-Polyplexes
Reinhard, S., Wang, Y., Dengler, S., & Wagner, E. (2018) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.8b00585 · Cited by 13
De, A., & Ko, Y. T. (2022) · Drug Delivery · doi:10.1080/10717544.2022.2108523 · Cited by 13
Yang, Q., Dong, Y., Wang, X., Lin, Z., Yan, M., Wang, W., Dong, A., Zhang, J., Huang, P., & Wang, C. (2021) · Macromolecular Bioscience · doi:10.1002/mabi.202100025 · Cited by 13
Improving the Delivery of Drugs and Nucleic Acids to T Cells Using Nanotechnology
Lou, J. W. H., Heater, A., & Zheng, G. (2021) · Small Structures · doi:10.1002/sstr.202100026 · Cited by 13
Acid‐Responsive Polymer Additives Increase RNA Transfection from Lipid Nanoparticles
Cheung, T. H., Fuchs, A., & Shoichet, M. S. (2024) · Advanced Functional Materials · doi:10.1002/adfm.202413220 · Cited by 13
Nagaraj, H., Lehot, V., Nasim, N., Çiçek, Y. A., Goswami, R., Jeon, T., & Rotello, V. M. (2025) · RSC Pharmaceutics · doi:10.1039/d5pm00129c · Cited by 12
Spatial Conformation of Ionizable Lipids Regulates Endosomal Membrane Disruption
Zhao, Y., Qu, Z., Paloncýová, M., Wang, Z., Weng, B., Zhao, Y., Liu, L., Song, D., Wich, D., Otyepka, M., et al. (2025) · Journal of the American Chemical Society · doi:10.1021/jacs.5c10908 · Cited by 13
Lipid-Based Intelligent Vehicle Capabilitized with Physical and Physiological Activation
Zhang, F., Xia, B., Sun, J., Wang, Y., Wang, J., Xu, F., Chen, J., Lu, M., Yao, X., Timashev, P. S., et al. (2022) · Research · doi:10.34133/2022/9808429 · Cited by 12
Shaharyar, M. A., Bhowmik, R., Al-Abbasi, F. A., AlGhamdi, S. A., Alghamdi, A. M., Sarkar, A., Kazmi, I. A., & Karmakar, S. (2023) · Vaccines · doi:10.3390/vaccines11020241 · Cited by 12
Salvador, C., Andreozzi, P., Romero, G., Loinaz, I., Dupin, D., & Moya, S. E. (2023) · ACS Applied Bio Materials · doi:10.1021/acsabm.2c00845 · Cited by 12
Synthetic Ionizable Colloidal Drug Aggregates Enable Endosomal Disruption
Donders, E. N., Slaughter, K. V., Dank, C., Ganesh, A. N., Shoichet, B. K., Lautens, M., & Shoichet, M. S. (2023) · Advanced Science · doi:10.1002/advs.202300311 · Cited by 12
Castanotto, D., Lin, M., Kowolik, C. M., Koch, T., Hansen, B. R., Oerum, H., & Stein, C. A. (2016) · Molecular Therapy · doi:10.1038/mt.2016.54 · Cited by 12
Artificial peptides for antitumoral siRNA delivery
Luo, J., Wagner, E., & Wang, Y. (2020) · Journal of Materials Chemistry B · doi:10.1039/c9tb02756d · Cited by 12
Kay, E. I., Stulz, R., Becquart, C., Lovrić, J., Tängemo, C., Thomen, A., Baždarević, D., Najafinobar, N., Dahlén, A., Pielach, A., et al. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14020463 · Cited by 12
A multiobjective AI model for LNP engineering enhances tissue-selective mRNA delivery
Zhou, M., Xu, Y., Li, G., Chen, J., Savguira, M., Seto, B. Y., Gong, F., Thomson, T., Chen, J., Lu, R. X. Z., et al. (2026) · Nature Biotechnology · doi:10.1038/s41587-026-03109-0 · Cited by 11
Cheng, Y., Zhao, E., Yang, X., Luo, C., Zi, G., Wang, R., Xu, Y., & Peng, B. (2024) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2024.125024 · Cited by 11
Exploring the promise of lipoplexes: From concept to clinical applications
Al-Shihabi, A. M., Al-Mohaya, M., Haider, M. A., & Demiralp, B. (2025) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2025.125424 · Cited by 11
Membrane-modified lipid nanoparticles for RNA delivery
Chowdhury, C. R., Hoover, E. C., & Day, E. S. (2025) · Molecular Therapy — Methods & Clinical Development · doi:10.1016/j.omtm.2025.101505 · Cited by 11
Lau, Y. M. A., Pang, J., Tilstra, G., Couture‐Senécal, J., & Khan, O. F. (2022) · Expert Opinion on Drug Delivery · doi:10.1080/17425247.2022.2144827 · Cited by 11
Archaeal ether lipids improve internalization and transfection with mRNA lipid nanoparticles
Sedlmayr, V. L., Schobesberger, S., Spitz, S., Ertl, P., Wurm, D. J., Quehenberger, J., & Oliver Spadiut, A. (2024) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2024.114213 · Cited by 11
Intracellular miRNA or siRNA delivery and function
Zhdanov, V. P. (2018) · Biosystems · doi:10.1016/j.biosystems.2018.05.007 · Cited by 11
Lv, W., & Champion, J. A. (2020) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2020.102315 · Cited by 11
Wang, J., Yu, L., Zhou, A., Liu, J., Wang, K., Luo, Y., & Wang, F. (2021) · Pharmaceutics · doi:10.3390/pharmaceutics13091520 · Cited by 11
Gao, D., Xu, Z., Li, X., Zhao, Y., Min, Q., Chen, Z., Xu, Q., Tian, Y., Xu, J., & Zhu, J. (2025) · Angewandte Chemie International Edition · doi:10.1002/anie.202505290 · Cited by 11
Lipid nanoparticles with prazole adjuvant to enhance the efficacy of mRNA cancer vaccines
Kim, Y. A., Jeong, H., Kim, H., Lee, S., Kim, K. S., & Na, K. (2025) · Journal of Controlled Release · doi:10.1016/j.jconrel.2025.113756 · Cited by 10
Sjöberg, M., Olsén, E., Mapar, M., Parkkila, P., Niederkofler, S., Mohammadi, S., Jing, Y., Emilsson, G., Lindfors, L., Agnarsson, B., et al. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2426601122 · Cited by 10
Narum, S. M., Zhang, J., Vo, B., Mancuso, J. N., & Salaita, K. S. (2025) · ACS Nano · doi:10.1021/acsnano.5c00177 · Cited by 10
VirPorters: Insights into the action of cationic and histidine-rich cell-penetrating peptides
Váňová, J., Číhařová, B., Hejtmánková, A., Epperla, C. P., Škvára, P., Forstová, J., Kalbáčová, M. H., & Španielová, H. (2021) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2021.121308 · Cited by 10
mRNA-Based Cancer Vaccines: Advancements and Prospects
Wu, L., Yi, W., Yao, S., Xie, S., Peng, R., Zhang, J., & Tan, W. (2024) · Nano Letters · doi:10.1021/acs.nanolett.4c03296 · Cited by 10
Blersch, J., Francisco, V., Rebelo, C., Jiménez‐Balsa, A., Antunes, H., Pinto, S., Simões, S. M. N., Rai, A., & Ferreira, L. (2020) · Nanoscale · doi:10.1039/c9nr10503d · Cited by 10
Revival of Bioengineered Proteins as Carriers for Nucleic Acids
Scherer, D., Burger, M., & Leroux, J. (2024) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.4c00079 · Cited by 10
Jagaran, K., Habib, S., & Singh, M. (2024) · Polymers · doi:10.3390/polym16233265 · Cited by 9
Malong, L., Roskosch, J., Hager, C., Fortin, J., Schmucki, R., Callow, M. G., Weile, C., Romeo, V., Patsch, C., Martin, S. E., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-61039-y · Cited by 10
Impact of Nanoparticle Stiffness on Endosomal Escape and Signaling Pathways in Cytosolic Delivery
Zhang, Y., Hui, Y., Guo, Z., Liu, D., Liu, Y., Gao, H., & Zhao, C. (2025) · Advanced Healthcare Materials · doi:10.1002/adhm.202501706 · Cited by 10
Zhang, Y., Li, J., Wang, Z., Kuang, Y., Li, S., & Wang, X. (2025) · Animal Diseases · doi:10.1186/s44149-025-00186-7 · Cited by 9
Iqbal, S., Luo, B., Melamed, J. R., & Day, E. S. (2021) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.1c00405 · Cited by 9
Targeting the Inside of Cells with Biologicals: Toxin Routes in a Therapeutic Context
Ruschig, M., & Marschall, A. L. J. (2023) · BioDrugs · doi:10.1007/s40259-023-00580-y · Cited by 9
Merckx, P., Conickx, G., Blomme, E. E., Maes, T., Bracke, K. R., Brusselle, G., Smedt, S. C. D., & Raemdonck, K. (2024) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2024.114223 · Cited by 9
Solid-phase supported design of carriers for therapeutic nucleic acid delivery
Levačić, A. K., Morys, S., & Wagner, E. (2017) · Bioscience Reports · doi:10.1042/bsr20160617 · Cited by 9
Interdisciplinary advances reshape the delivery tools for effective NASH treatment
Chen, L., & Wang, Y. (2023) · Molecular Metabolism · doi:10.1016/j.molmet.2023.101730 · Cited by 9
Hayashi, Y., Higashi, T., Motoyama, K., Jono, H., Ando, Y., & Arima, H. (2017) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2017.09.085 · Cited by 9
Ji, Q., Kowalski, K. P., Golenberg, E. M., Chung, S. H., Barker, N. D., Bickford, W. A., & Gong, P. (2025) · Plants · doi:10.3390/plants14030458 · Cited by 8
Chen, L., Bosmajian, C., & Woo, S. (2025) · Molecular Therapy — Nucleic Acids · doi:10.1016/j.omtn.2025.102516 · Cited by 9
Cui, S., Wu, X., Yu, X., Huang, Y., Zhang, D., Chen, H., Heng, B. C., Cai, Q., Wang, Y., Gu, Y., et al. (2025) · Advanced Materials · doi:10.1002/adma.202410507 · Cited by 10
Egberink, R. O., van Schie, D. M., Joosten, B., de Muynck, L. T. A., Jacobs, W., van Oostrum, J., & Brock, R. E. (2024) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2024.114414 · Cited by 8
Advancing the use of noncoding RNA in regulatory toxicology: Report of an ECETOC workshop
Aigner, A. M., Buesen, R., Gant, T. W., Gooderham, N. J., Greim, H. A., Jörg Hackermüller, A., Hubesch, B., Laffont, M., Marczylo, E. L., Meister, G., et al. (2016) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2016.09.018 · Cited by 8
Liang, M., Cheng, Y., & Wang, H. (2023) · Chemistry - A European Journal · doi:10.1002/chem.202300131 · Cited by 8
Francisco, V., Rebelo, C., Rodrigues, A. F., Blersch, J., Fernandes, H., & Ferreira, L. (2020) · Methods · doi:10.1016/j.ymeth.2020.12.002 · Cited by 8
Wang, D., Wang, X., Wang, L., Zhang, J., Ma, J., Xia, G., & Hong, B. (2021) · European Journal of Pharmaceutical Sciences · doi:10.1016/j.ejps.2021.105803 · Cited by 8
Cationic lipid nanoparticles for nucleic acid delivery: microfluidics versus thin film hydration
Park, H., Lee, J., Kang, J.-W., Min, J.-Y., Lee, J., Hong, J., & Shim, G. (2025) · Journal of Pharmaceutical Investigation · doi:10.1007/s40005-025-00737-7 · Cited by 7
Knaggs, K. L. M., Sun, Y., Walz, B. A., Pang, J., & Khan, O. F. (2025) · Therapeutic Delivery · doi:10.1080/20415990.2025.2506977 · Cited by 7
Zhang, H., Li, Y., Li, J., Li, X., & Li, T. (2025) · Pharmaceutics · doi:10.3390/pharmaceutics17111405 · Cited by 8
Design of messenger RNA vaccines based on lipid-polymer hybrid nanoparticles
Baghel, S. S., Lokras, A. G., Dias, B. A., Landry, M. R., Cérda, S. L., Wadhwa, A., Herrera‐Barrera, M., Thakur, A., Rades, T., Franzyk, H., et al. (2025) · Journal of Controlled Release · doi:10.1016/j.jconrel.2025.114385 · Cited by 7
Intracellular Communication between Synthetic Macromolecules
Evans, C. W., Ho, D., Marlow, J. B., King, J. J., Hee, C., Wong, L. N., Atkin, R., Smith, N. M., Warr, G. G., Nörret, M., et al. (2022) · Journal of the American Chemical Society · doi:10.1021/jacs.2c02793 · Cited by 7
Dave, P., Anand, P., Kothawala, A., Srikaram, P., SHASTRI, D. D., Uddin, A., Bhavsar, J., & Winer, A. (2024) · Cureus · doi:10.7759/cureus.62981 · Cited by 8
Biotransformation novel advances – 2021 year in review
Khojasteh, S. C., Argikar, U. A., Cho, S., Crouch, R. D., Heck, C. J. S., Johnson, K. M., Kalgutkar, A. S. S., King, L. M., Maw, H. H., Seneviratne, H. K., et al. (2022) · Drug Metabolism Reviews · doi:10.1080/03602532.2022.2097253 · Cited by 7
Chang, R., Yan, Q., Kingshott, P., Tsai, W., & Wang, P. (2020) · Acta Biomaterialia · doi:10.1016/j.actbio.2020.05.015 · Cited by 8
Zhang, Y., Dong, Y., Zhou, J., Huang, W., Wu, Y., Zhao, S., Shi, Y., Bai, S., Li, C., Huang, Y., et al. (2021) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2021.112178 · Cited by 7
Greco, C. T., Epps, T. H. I., & Sullivan, M. O. (2017) · Journal of Visualized Experiments · doi:10.3791/55803 · Cited by 7
The RNA delivery dilemma—lipid versus polymer nanoparticle platforms
Martins, C., Mitchell, M. J., Peer, D., Perrie, Y., Siegwart, D. J., Alonso, M. J., & Aparicio-Blanco, J. (2026) · Drug Delivery and Translational Research · doi:10.1007/s13346-026-02044-6 · Cited by 10
Sorets, A. G., Schwensen, K. R., Francini, N., Kjar, A., Abdulrahman, A. M., Shostak, A., Katdare, K. A., Schoch, K. M., Cowell, R. P., Park, J. C., et al. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf600 · Cited by 6
“In addition, siRNAs are more potent on a per-molecule basis compared to ASO; ∼ 2000 cytoplasmic siRNAs are estimated to achieve maximal silencing, whereas ∼ 50 000 are required for an ASO [ 54 , 55 ].”
Lee, S., Park, J., Jung, H. N., Li, S., Lin, Z., & Im, H. (2025) · Advanced Healthcare Materials · doi:10.1002/adhm.202503055 · Cited by 7
Xu, Z., Cheng, X., Guo, X., Li, P., Li, J., Zhao, J., & Wang, Z. (2025) · Angewandte Chemie International Edition · doi:10.1002/anie.202516456 · Cited by 6
A machine learning benchmarking framework for lipid nanoparticle transfection efficiency prediction
Mehradfar, A., Sepehri, M. S., Hernández-Lobato, J. M., Kwon, G. S., Soltanolkotabi, M., Avestimehr, S., & Rasoulianboroujeni, M. (2026) · Communications AI & Computing · doi:10.1038/s44488-026-00007-x · Cited by 6
Samanta, A., Malle, M. G., Tsang, E., Omer, M., Skaanning, M. K., Youssef, S., Kjems, J., & Gothelf, K. V. (2026) · Advanced Science · doi:10.1002/advs.202512844 · Cited by 6
Conti, L., Rojas‐Rodríguez, M., Caselli, L., Bonavolontà, D., Sanità, G., Esposito, E., Calamai, M., Marradi, M., Berti, D., & Montis, C. (2026) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2026.139966 · Cited by 6
Truong, H. Q., Kafle, U., Cao, T. G. N., Li, Y., & Meng, F. (2026) · Journal of Nanobiotechnology · doi:10.1186/s12951-026-04383-5 · Cited by 6
Delivery Vehicles for Self-amplifying RNA
Bathula, N. V., Popova, P. G., & Blakney, A. K. (2022) · RNA technologies · doi:10.1007/978-3-031-08415-7_16 · Cited by 6
Yu, S., Zheng, J., Xie, H., Deng, Q., Wang, J., Chen, J., Zhou, H., & Wang, J. (2024) · ACS Applied Nano Materials · doi:10.1021/acsanm.4c00466 · Cited by 6
Ionizable Lipids from Click Reactions for Lipid Nanoparticle Assembling and mRNA Delivery
Xu, F., Si, X., Wang, Y., Sun, C., Liu, M., Zhang, Y., Xu, X., & Tian, T. (2024) · The Journal of Physical Chemistry B · doi:10.1021/acs.jpcb.3c07600 · Cited by 7
The role and research progress of macrophages after heart transplantation
Chen, Y., Wang, J., An, C., Bao, S., & Zhang, C. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e33844 · Cited by 7
Norimatsu, J., Hashim, R., Tockary, T. A., Nazir, M. S., Mochida, Y., & Uchida, S. (2025) · ACS Applied Nano Materials · doi:10.1021/acsanm.5c00633 · Cited by 7
Epanchintseva, A. V., Poletaeva, J., Dome, A. S., Dovydenko, I. S., Pyshnaya, I. A., & Ryabchikova, E. I. (2022) · Nanomaterials · doi:10.3390/nano12244450 · Cited by 6
Roh, E. H., Sullivan, M. O., & Epps, T. H. I. (2024) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.3c15424 · Cited by 6
Advanced molecular imaging for the characterisation of complex medicines
Yilmaz, D., Sharp, P. S., Main, M. J., & Simpson, P. B. (2022) · Drug Discovery Today · doi:10.1016/j.drudis.2022.03.001 · Cited by 5
Polymeric Gene Delivery at Sub-Stoichiometric N/P Ratios
Wu, Y. C., Cheng, X., Wang, Y., Wang, H., Cheng, Y., & Lv, J. (2025) · Polymer science & technology. · doi:10.1021/polymscitech.5c00095 · Cited by 5
Yang, N., Yan, Y., Li, Z., Lu, A., Li, L., Zhu, Y., Shi, Y., Liu, X.-Y., & Wang, J. (2025) · Journal of Controlled Release · doi:10.1016/j.jconrel.2025.114378 · Cited by 5
Self-citation
Metadata
- Authors
- Wittrup, A., Ai, A., Liu, X., Hamar, P., Trifonova, R., Charisse, K., Manoharan, M., Kirchhausen, T., & Lieberman, J.
- Affiliations
- Boston Children's Hospital (US)
- Alnylam Pharmaceuticals (United States) (US)
- Corresponding author
- Wittrup, Anders
- Lieberman, Judy
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2015-07-20
- Volume
- 33
- Issue
- 8
- Pages
- 870-876
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC4663660
- OpenAlex
- W1844274376
- Cited by
- 693
- References
- 30
- Citations by year
- 2026: 101 · 2025: 104 · 2024: 82 · 2023: 60 · 2022: 59 · 2021: 63 · 2020: 53 · 2019: 40 · 2018: 54 · 2017: 37 · 2016: 31 · 2015: 8
- FWCI
- 18.70
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Lund University Publications (Lund University) (submittedVersion)
- Funders
- National Institutes of Health
- National Cancer Institute (R01CA139444, CA139444)
- National Institute of General Medical Sciences (GM075252, R01GM075252)
- National Institute of Allergy and Infectious Diseases (AI090671, AI057159, R01AI090671, U54AI057159)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- siRNA delivery, endosomal escape, lipoplexes, lipid nanoparticles, endocytosis, autophagy, galectins, gene knockdown, live-cell imaging, RNA interference, siRNA therapeutics
- MeSH terms
- HeLa Cells, Humans, Lipids, Luminescent Proteins, Endosomes, RNA Interference, RNA, Small Interfering, Nanoparticles, Gene Knockdown Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review