The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow
Open accessAbstract
A lack of techniques to image multiple genomic loci in living cells has limited our ability to investigate chromosome dynamics. Here we describe CRISPRainbow, a system for labeling DNA in living cells based on nuclease-dead (d) Cas9 combined with engineered single guide RNA (sgRNA) scaffolds that bind sets of fluorescent proteins. We demonstrate simultaneous imaging of up to six chromosomal loci in individual live cells and document large differences in the dynamic properties of different chromosomal loci.
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Cites
Development and Applications of CRISPR-Cas9 for Genome Engineering
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Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
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Improving CRISPR-Cas nuclease specificity using truncated guide RNAs
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Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System
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Engineering complex synthetic transcriptional programs with CRISPR RNA scaffolds
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Orthogonal Cas9 proteins for RNA-guided gene regulation and editing
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A Cas9–guide RNA complex preorganized for target DNA recognition
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Advances in fluorescence labeling strategies for dynamic cellular imaging
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Multicolor CRISPR labeling of chromosomal loci in human cells
Ma, H., Naseri, A., Reyes-Gutierrez, P., Wolfe, S. A., Zhang, S., & Pederson, T. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1420024112 · Cited by 412
Self-citationCas9 gRNA engineering for genome editing, activation and repression
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Cited by
The CRISPR tool kit for genome editing and beyond
Adli, M. (2018) · Nature Communications · doi:10.1038/s41467-018-04252-2 · Cited by 1,545
The next generation of CRISPR–Cas technologies and applications
Pickar-Oliver, A., & Gersbach, C. A. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0131-5 · Cited by 1,459
Applications of CRISPR technologies in research and beyond
Barrangou, R., & Doudna, J. A. (2016) · Nature Biotechnology · doi:10.1038/nbt.3659 · Cited by 1,005
Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells
Bintu, B., Mateo, L. J., Su, J.-H., Sinnott-Armstrong, N. A., Parker, M., Kinrot, S., Yamaya, K., Boettiger, A. N., & Zhuang, X. (2018) · Science · doi:10.1126/science.aau1783 · Cited by 976
CRISPR/Cas Systems towards Next-Generation Biosensing
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Metadata
- Authors
- Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., & Pederson, T.
- Venue
- Nature Biotechnology
- Published
- 2016-04-18
- Volume
- 34
- Issue
- 5
- Pages
- 528-530
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2336531194
- Cited by
- 470
- References
- 18
- FWCI
- 24.98
- Type
- journal article
- Language
- en
- Open access
- green
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- Ma, H., Tu, L.-C., Naseri, A., Huisman, M., Zhang, S., Grunwald, D., & Pederson, T. (2016). Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nature Biotechnology. https://doi.org/10.1038/nbt.3526
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