The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
A Cas9–guide RNA complex preorganized for target DNA recognition
Abstract
An RNA seed poised to meet its target: The CRISPR-Cas system in prokaryotes precisely identifies infecting parasitic DNAs and viruses and destroys them. The CRISPR-Cas system has been adapted for facile genome editing, heralding a new age in molecular biology. Jiang et al. show that the Cas9 nuclease adopts a distinct confirmation when it binds to the targeting guide RNA. The guide RNA then assumes a preordered shape. This RNA “seed region” is thus poised to initiate recognition of the DNA target sequence. Science , this issue p. 1477
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Self-citationMultiplex Genome Engineering Using CRISPR/Cas Systems
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Cited by
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Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., van der Oost, J., Regev, A., et al. (2015) · Cell · doi:10.1016/j.cell.2015.09.038 · Cited by 4,761
High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects
Kleinstiver, B. P., Pattanayak, V., Prew, M. S., Tsai, S. Q., Nguyen, N. T., Zheng, Z., & Joung, J. K. (2016) · Nature · doi:10.1038/nature16526 · Cited by 2,673
Crystal structure of Cas9 in complex with guide RNA and target DNA
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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Anzalone, A. V., Koblan, L. W., & Liu, D. R. (2020) · Nature Biotechnology · doi:10.1038/s41587-020-0561-9 · Cited by 2,237
CRISPR–Cas9 Structures and Mechanisms
Jiang, F., & Doudna, J. A. (2017) · Annual Review of Biophysics · doi:10.1146/annurev-biophys-062215-010822 · Cited by 2,029
Self-citation
Metadata
- Authors
- Jiang, F., Zhou, K., Ma, L., Gressel, S., & Doudna, J. A.
- Venue
- Science
- Published
- 2015-06-26
- Volume
- 348
- Issue
- 6242
- Pages
- 1477-1481
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2124812694
- Cited by
- 563
- References
- 15
- FWCI
- 23.41
- Type
- journal article
- Language
- en
- Open access
- closed
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