Cited by 1,358Insect Resistance and GeneticsInsect-Plant Interactions and ControlInsect Pest Control Strategies

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Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol

Mao, Ying-Bo, Cai, Wen-Juan, Wang, Jia-Wei, Hong, Gao-Jie, Tao, Xiao-Yuan, Wang, Ling-Jian, Huang, Yong-Ping, Chen, Xiao-Ya·2007·Nature Biotechnology

DOI 10.1038/nbt1352

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Abstract

We identify a cytochrome P450 gene (CYP6AE14) from cotton bollworm (Helicoverpa armigera), which permits this herbivore to tolerate otherwise inhibitory concentrations of the cotton metabolite, gossypol. CYP6AE14 is highly expressed in the midgut and its expression correlates with larval growth when gossypol is included in the diet. When larvae are fed plant material expressing double-stranded RNA (dsRNA) specific to CYP6AE14, levels of this transcript in the midgut decrease and larval growth is retarded. Both effects are more dramatic in the presence of gossypol. As a glutathione-S-transferase gene (GST1) is silenced in GST1 dsRNA-expressing plants, feeding insects plant material expressing dsRNA may be a general strategy to trigger RNA interference and could find applications in entomological research and field control of insect pests.

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Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana
Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 22,014
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Specific interference by ingested dsRNA
Timmons, L., & Fire, A. Z. (1998) · Nature · doi:10.1038/27579 · Cited by 1,938
Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
Timmons, L., Court, D. L., & Fire, A. Z. (2001) · Gene · doi:10.1016/s0378-1119(00)00579-5 · Cited by 1,840
Genetic and Functional Diversification of Small RNA Pathways in Plants
Xie, Z., Johansen, L. K., Gustafson, A. M., Kasschau, K. D., Lellis, A. D., Zilberman, D., Jacobsen, S. E., & Carrington, J. C. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020104 · Cited by 1,569
Construct design for efficient, effective and high‐throughput gene silencing in plants
Wesley, S. V., Helliwell, C. A., Smith, N. A., Wang, M., Rouse, D. T., Liu, Q., Gooding, P. S., Singh, S. P., Abbott, D., Stoutjesdijk, P. A., et al. (2001) · The Plant Journal · doi:10.1046/j.1365-313x.2001.01105.x · Cited by 1,542
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Tabara, H., Sarkissian, M., Kelly, W. G., Fleenor, J., Grishok, A., Timmons, L., Fire, A. Z., & Mello, C. C. (1999) · Cell · doi:10.1016/s0092-8674(00)81644-x · Cited by 1,307
Superoxide radical inhibits catalase.
Kono, Y., & Fridovich, I. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83842-5 · Cited by 1,254
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Winston, W. M., Molodowitch, C., & Hunter, C. P. (2002) · Science · doi:10.1126/science.1068836 · Cited by 849
Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline
Aravin, A. A., Nm, N., Tulin, A. V., Vagin, V. V., Rozovsky, Y. M., & Gvozdev, V. A. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00299-8 · Cited by 827

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Background
Insect resistance management in Bacillus thuringiensis cotton by MGPS (multiple genes pyramiding and silencing)
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Background
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Background
Role of RNA interference in plant improvement
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Background
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Background
Immunity or Digestion
Pauchet, Y., Freitak, D., Heidel‐Fischer, H. M., Heckel, D. G., & Vogel, H. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m806204200 · Cited by 91
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Background
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Method
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BackgroundInfluential
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Background
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BackgroundMethod
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Background
Establishing an In Vivo Assay System to Identify Components Involved in Environmental RNA Interference in the Western Corn Rootworm
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Background
Transgenic plants over-expressing insect-specific microRNA acquire insecticidal activity against Helicoverpa armigera: an alternative to Bt-toxin technology
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Background
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Background
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Method
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Background
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Background
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“In the control of plant viral diseases, transgenic plants expressing dsRNA or siRNA that target components in the vector insects have been shown to be promising (Baum et al., 2007; Mao et al., 2007; Thakur et al., 2014).”
Gossypol: phytoalexin of cotton
Tian, X., Ruan, J.-X., Huang, J., Fang, X., Mao, Y., Wang, L.-J., Chen, X., & Yang, C. (2016) · Science China Life Sciences · doi:10.1007/s11427-016-5003-z · Cited by 84
“When suppressed by plant mediated insect RNA interference (RNAi) approach, decreased gossypol tolerance of bollworm was observed (Mao et al., 2007), and transgenic cotton plants expressing the double stranded RNA against GIP (dsGIP) acquired enhanced resistance to cotton bollworms (Mao et al.,…”
Background
A Transmissible RNA Pathway in Honey Bees
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BackgroundInfluential
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Background
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BackgroundMethodInfluential
A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey
Oberemok, V. V., Laikova, K. V., Repetskaya, A., Kenyo, I., Владимирович, Г. М., Касич, И. Н., Krasnodubets, A. M., Gal’chinsky, N. V., Фомочкина, И. И., Zaitsev, A. S., et al. (2018) · Molecules · doi:10.3390/molecules23061302 · Cited by 77
“Along with using RNAi to develop therapies for human diseases, one of the earliest non-research applications envisaged was the use of plant expressed dsRNA to confer resistance to insects; the first reports of its effectiveness were in 2007 [26,97].”
Background
Mechanisms of resistance to thiamethoxam and dinotefuran compared to imidacloprid in the brown planthopper: Roles of cytochrome P450 monooxygenase and a P450 gene CYP6ER1
Sun, X., Gong, Y., Ali, S., et al. (2018) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2018.06.014 · Cited by 77
Efficient control of western flower thrips by plastid-mediated RNA interference
Wu, M., Dong, Y., Zhang, Q., Li, S., Chang, L., Loiacono, F. V., Ruf, S., Zhang, J., & Bock, R. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2120081119 · Cited by 77
“Nuclear transgenic plants expressing dsRNA against insect essential genes have been proven to confer a limited level of protection from several insects, including the cotton bollworm (Helicoverpa armigera) (1, 2), the western corn rootworm (Diabrotica virgifera) (3, 4), the brown planthopper (Nilaparvata lugens) (5),…”
Background
The possible impact of persistent virus infection on the function of the RNAi machinery in insects: a hypothesis
Swevers, L., Broeck, J. V., & Smagghe, G. J. (2013) · Frontiers in Physiology · doi:10.3389/fphys.2013.00319 · Cited by 83
“Environmental RNAi has also been used to induce toxic effects in the cotton bollworm, Helicoverpa armigera (Mao et al., 2007).”
Background
A Comparative miRNAome Analysis Reveals Seven Fiber Initiation-Related and 36 Novel miRNAs in Developing Cotton Ovules
Wang, Z., Xue, W., Dong, C., et al. (2011) · Molecular Plant · doi:10.1093/mp/ssr094 · Cited by 77
Study on the Effect of Wing Bud Chitin Metabolism and Its Developmental Network Genes in the Brown Planthopper, Nilaparvata lugens, by Knockdown of TRE Gene
Zhang, L., Qiu, L.-Y., Yang, H., et al. (2017) · Frontiers in Physiology · doi:10.3389/fphys.2017.00750 · Cited by 77
“…effective RNAi expressing dsRNA in the host plant or transgenemediated RNAi developed to decrease gene expression at the same time will be very useful for pest control (Baum et al., 2007; Mao et al., 2007; Swevers et al., 2013; Zhu, 2013; Jiang et al., 2016; Yu et al., 2016; Gillet et al., 2017).”
Background
Nucleases as a barrier to gene silencing in the cotton boll weevil, Anthonomus grandis
Garcia, R. A., Macedo, L. L. P., Cabral, D., Gillet, F., Pinto, C. E. M., Faheem, M. Q., Basso, A. M. M., Silva, M. C. M., & Grossi‐de‐Sá, M. F. (2017) · PLoS ONE · doi:10.1371/journal.pone.0189600 · Cited by 78
RNA interference in insects: the link between antiviral defense and pest control
Niu, J., Chen, R., & Wang, J. (2023) · Insect Science · doi:10.1111/1744-7917.13208 · Cited by 76
“The concept of RNAi-based insect pest control was first applied in beetles and moths, aiming to use ingested dsRNA to evoke the RNAi response against genes that are essential for insect development or survival (Baum et al. , 2007; Mao et al. , 2007).”
Background
De novo intestine-specific transcriptome of the brown planthopper Nilaparvata lugens revealed potential functions in digestion, detoxification and immune response
Bao, Y., Wang, Y., Wu, W.-J., et al. (2012) · Genomics · doi:10.1016/j.ygeno.2012.02.002 · Cited by 76
Development of an Efficient Recombinant Mosquito Densovirus-Mediated RNA Interference System and Its Preliminary Application in Mosquito Control
Gu, J., Liu, M., Deng, Y., Peng, H., & Chen, X. (2011) · PLoS ONE · doi:10.1371/journal.pone.0021329 · Cited by 76
CYP9A12 and CYP9A17 in the cotton bollworm, Helicoverpa armigera: sequence similarity, expression profile and xenobiotic response
Zhou, X., Ma, C., Li, M., Sheng, C.-F., Liu, H., & Qiu, X. (2009) · Pest Management Science · doi:10.1002/ps.1832 · Cited by 76
Global trends in research and commercialization of exogenous and endogenous RNAi technologies for crops
Jalaluddin, N. S. M., Othman, R. Y., & Harikrishna, J. A. (2018) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2018.1496064 · Cited by 75
“The most cited article was published in Nature Biotechnology, and described the development of an RNAi strategy to kill cotton bollworms Helicoverpa armigera by inserting a dsRNA construct containing a 35S promoter, a sense fragment of CYP6AE14 cDNA, a 120-nucleotide intron, an antisense fragment of CYP6AE14 cDNA, and…”
Background
UDP-glycosyltransferase genes and their association and mutations associated with pyrethroid resistance in Anopheles sinensis (Diptera: Culicidae)
Zhou, Y., Fu, W., Si, F., Yan, Z., Zhang, Y., He, Q., & Chen, B. (2019) · Malaria Journal · doi:10.1186/s12936-019-2705-2 · Cited by 71
Chitosan nanocarriers mediated dsRNA delivery in gene silencing for Helicoverpa armigera biocontrol
Kolge, H., Kadam, K., & Ghormade, V. (2022) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2022.105292 · Cited by 75
Aphid resistance in Brassica crops: challenges, biotechnological progress and emerging possibilities
Bhatia, V., Uniyal, P. L., & Bhattacharya, R. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2011.07.005 · Cited by 75
Using double-stranded RNA to explore the role of heat shock protein genes in heat tolerance in Bemisia tabaci (Gennadius)
Lü, Z., & Wan, F. (2011) · Journal of Experimental Biology · doi:10.1242/jeb.047415 · Cited by 74
“In contrast, the dsRNA feeding method is simple and easy to do (Baum et al., 2007; Mao et al., 2007).”
Method
Ingested plant miRNAs regulate gene expression in animals
Vaucheret, H., & Chupeau, Y. (2011) · Cell Research · doi:10.1038/cr.2011.164 · Cited by 75
RNA Interference based Approach to Down Regulate Osmoregulators of Whitefly (Bemisia tabaci): Potential Technology for the Control of Whitefly
Raza, A., Malik, H. J., Shafiq, M., et al. (2016) · PLoS ONE · doi:10.1371/journal.pone.0153883 · Cited by 74
“Some of the examples include cytochrome P450 gene inHelicoverpa armigera [25], V-ATPase gene in Diabrotica virgifera virgifera [26] and aquaporin and C002 genes in Acyrthosiphon pisum [27, 28].”
Background
Clathrin-dependent endocytosis is associated with RNAi response in the western corn rootworm, Diabrotica virgifera virgifera LeConte
Pinheiro, D. H., Vélez, A. M., Fishilevich, E., et al. (2018) · PLoS ONE · doi:10.1371/journal.pone.0201849 · Cited by 74
Recent trends and advances of RNA interference (RNAi) to improve agricultural crops and enhance their resilience to biotic and abiotic stresses
Bharathi, J. K., Anandan, R., Benjamin, L. K., Muneer, S., & Prakash, M. (2022) · Plant Physiology and Biochemistry · doi:10.1016/j.plaphy.2022.11.035 · Cited by 75
Induced resistance to Helicoverpa armigera through exogenous application of jasmonic acid and salicylic acid in groundnut, Arachis hypogaea
War, A. R., Paulraj, M. G., Savarimuthu Ignacimuthu, S. J., & Sharma, H. C. (2014) · Pest Management Science · doi:10.1002/ps.3764 · Cited by 74
Transcriptome Analysis and Screening for Potential Target Genes for RNAi-Mediated Pest Control of the Beet Armyworm, Spodoptera exigua
Li, H., Jiang, W., Zhang, Z., Xing, Y., & Li, F. (2013) · PLoS ONE · doi:10.1371/journal.pone.0065931 · Cited by 74
“This breakthrough occurred in 2007, when RNAi-directed knock down of insect target genes in transgenic plants was shown to protect plants from coleopteran and lepidopteran pests, opening a new era for generation of insectresistant crops [22,23].”
Background
Selection of reference genes for RT-qPCR analysis in a predatory biological control agent, Coleomegilla maculata (Coleoptera: Coccinellidae)
Yang, C., Pan, H., Noland, J. E., Zhang, D., Zhang, Z., Liu, Y., & Zhou, X. J. (2015) · Scientific Reports · doi:10.1038/srep18201 · Cited by 81
Identification, mRNA Expression, and Functional Analysis of Chitin Synthase 1 Gene and Its Two Alternative Splicing Variants in Oriental Fruit Fly, Bactrocera dorsalis
Yang, W., Xu, K.-K., Cong, L., et al. (2013) · International Journal of Biological Sciences · doi:10.7150/ijbs.6022 · Cited by 73
“In recent years, RNA interference (RNAi)-based technology has shown great potential in controlling insect pests by silencing vital genes [24, 25].”
Background
Progress and prospects of arthropod chitin pathways and structures as targets for pest management
Liu, X., Cooper, A. M. W., Yu, Z., et al. (2019) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2019.08.002 · Cited by 72
Fitness Cost of Resistance to Bt Cotton Linked with Increased Gossypol Content in Pink Bollworm Larvae
Williams, J. L., Ellers‐Kirk, C., Orth, R. G., Gassmann, A. J., Head, G., Tabashnik, B. E., & Carrière, Y. (2011) · PLoS ONE · doi:10.1371/journal.pone.0021863 · Cited by 71
Transgenic Cotton Plants Expressing Double-stranded RNAs Target HMG-CoA Reductase (HMGR) Gene Inhibits the Growth, Development and Survival of Cotton Bollworms
Tian, G., Cheng, L., Qi, X., Ge, Z., Niu, C., Zhang, X., & Jin, S. (2015) · International Journal of Biological Sciences · doi:10.7150/ijbs.12463 · Cited by 71
“Feeding insect with transgenic plant leaves expressing dsRNA has been developed as an effective method to deliver dsRNA, and first successful application for cotton bollworm control was reported in 2007 [17].”
BackgroundMethod
RNA Interference in Moths: Mechanisms, Applications, and Progress
Xu, J., Wang, X., Chen, P., Fang-tao, L., Zheng, S., Ye, H., & Mo, M. (2016) · Genes · doi:10.3390/genes7100088 · Cited by 74
“Soon after the foundation by Fire et al. [5], RNAi was successfully applied to insects [9] and rapidly developed as a widely used tool in different insect orders thereafter, such as in Diptera, Coleoptera, Hymenoptera, and Lepidoptera (reviewed in [10]).”
BackgroundMethodInfluential
A transgenic strategy for controlling plant bugs (Adelphocoris suturalis) through expression of double‐stranded RNA homologous to fatty acyl‐coenzyme A reductase in cotton
Luo, J., Liang, S., Li, J., et al. (2017) · New Phytologist · doi:10.1111/nph.14636 · Cited by 71
Transgenic potato lines expressing hairpin RNAi construct of molting-associated EcR gene exhibit enhanced resistance against Colorado potato beetle (Leptinotarsa decemlineata, Say)
Hussain, T., Aksoy, E., Çalışkan, M. E., & Bakhsh, A. (2019) · Transgenic Research · doi:10.1007/s11248-018-0109-7 · Cited by 72
Salt Stress Effects on Secondary Metabolites of Cotton in Relation to Gene Expression Responsible for Aphid Development
Wang, Q., Eneji, A. E., Kong, X., Wang, K., & Dong, H. (2015) · PLoS ONE · doi:10.1371/journal.pone.0129541 · Cited by 69
“As a polyphenolic compound, (+)—and (-)—gossypol exhibits different levels of biological activities [5] and has biochemical effects onHelicoverpa armigeraHübner [10,11].”
Background
Endocytic pathway mediates refractoriness of insect Bactrocera dorsalis to RNA interference
Li, X., Dong, X., Zou, C. M., & Zhang, H. (2015) · Scientific Reports · doi:10.1038/srep08700 · Cited by 71
Characterization of a midgut-specific chitin synthase gene (LmCHS2) responsible for biosynthesis of chitin of peritrophic matrix in Locusta migratoria
Liu, X., Zhang, H., Li, S., et al. (2012) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2012.09.002 · Cited by 71
“Recently, transgenic plants engineered to produce hairpin dsRNAs in vivo have been reported for protecting the plants from the feeding damage of herbivorous insects (Baum et al., 2007; Gordon and Waterhouse, 2007; Mao et al., 2007).”
Background
RNA interference against gut osmoregulatory genes in phloem-feeding insects
Tzin, V., Yang, X., Jing, X., Zhang, K., Jander, G., & Douglas, A. E. (2015) · Journal of Insect Physiology · doi:10.1016/j.jinsphys.2015.06.006 · Cited by 71
Transcriptome Analysis in Cotton Boll Weevil (Anthonomus grandis) and RNA Interference in Insect Pests
Firmino, A., Fonseca, F. C. D. A., de Macedo, L. L. P., Coelho, R. R., de Souza, J. D. A., Togawa, R., Silva-Junior, O., Pappas-Jr, G. J., da Silva, M. C. M., Engler, G., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0085079 · Cited by 62
“Two groups reported GM plants that express dsRNA matching essential genes in the digestive tract of two important agricultural insect pests, the cotton bollworm Helicoverpa armigera (Lepidoptera) [26] and the western corn rootworm, Diabrotica virgifera virgifera (Coleoptera) [23].”
Background
A comparative evaluation of the regulation of GM crops or products containing dsRNA and suggested improvements to risk assessments
Heinemann, J. A., Agapito-Tenfen, S. Z., & Carman, J. A. (2013) · Environment International · doi:10.1016/j.envint.2013.02.010 · Cited by 69
Silencing NADPH-cytochrome P450 reductase results in reduced acaricide resistance in Tetranychus cinnabarinus (Boisduval)
Shi, L., Zhang, J., Shen, G., Xu, Z., Peng, W., Zhang, Y., Xu, Q., & He, L. (2015) · Scientific Reports · doi:10.1038/srep15581 · Cited by 70
Exchange of Small Regulatory RNAs between Plants and Their Pests
Hudzik, C., Hou, Y., Ma, W., et al. (2019) · PLANT PHYSIOLOGY · doi:10.1104/pp.19.00931 · Cited by 70
Gossypol toxicity and detoxification in Helicoverpa armigera and Heliothis virescens
Krempl, C., Heidel‐Fischer, H. M., Jiménez‐Alemán, G. H., et al. (2016) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2016.09.003 · Cited by 69
Phloroglucinol-Mediated Hsp70 Production in Crustaceans: Protection against Vibrio parahaemolyticus in Artemia franciscana and Macrobrachium rosenbergii
Kumar, V., Baruah, K., Nguyen, D. V., et al. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.01091 · Cited by 69
Duplex Structure of Double-Stranded RNA Provides Stability against Hydrolysis Relative to Single-Stranded RNA
Zhang, K., Hodge, J., Chatterjee, A., Moon, T. S., & Parker, K. M. (2021) · Environmental Science & Technology · doi:10.1021/acs.est.1c01255 · Cited by 71
Identifying potential RNAi targets in grain aphid (Sitobion avenae F.) based on transcriptome profiling of its alimentary canal after feeding on wheat plants
Zhang, M., Zhou, Y., Wang, H., et al. (2013) · BMC Genomics · doi:10.1186/1471-2164-14-560 · Cited by 68
Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L
Vélez, A. M., Jurzenski, J., Matz, N., Zhou, X. J., Wang, H., Ellis, M. D., & Siegfried, B. D. (2015) · Chemosphere · doi:10.1016/j.chemosphere.2015.09.068 · Cited by 68
“Empirical validation of the expression of hairpin RNA in plants argeting insect pests was first reported for the cotton bollworm, elicoverpa armigera (Hübner), (Mao et al., 2007) and the western orn rootworm (WCR), Diabrotica virgifera virgifera LeConte, 1868 Baum et al., 2007).”
Background

Authors: Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y.
Affiliations: Chinese Academy of Sciences (CN)
Corresponding author: Chen, Xiao-Ya
ORCID: Mao, Ying-Bo
Wang, Ling-Jian
Chen, Xiao-Ya
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2007-11
Volume: 25
Issue: 11
Pages: 1307-1313
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt1352
OpenAlex: W2121440246
Cited by: 1,358
References: 50
Citations by year: 2026: 44 · 2025: 76 · 2024: 80 · 2023: 56 · 2022: 111 · 2021: 98 · 2020: 84 · 2019: 87 · 2018: 67 · 2017: 95 · 2016: 92 · 2015: 89 · 2014: 84 · 2013: 77 · 2012: 68
FWCI: 13.10
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
Keywords: Helicoverpa armigera, cytochrome P450 monooxygenase, dsRNA feeding, midgut gene expression, larval growth, insect pest control, plant-insect interactions
MeSH terms: Animals, Gossypium, Cytochrome P-450 Enzyme System, Digestion, Drug Resistance, Glutathione Transferase, Gossypol, Larva, Lepidoptera, Molecular Sequence Data, Pest Control, Biological, RNA, Double-Stranded, Nicotiana, Arabidopsis, RNA, Plant, Insect Proteins, Gene Silencing, Plants, Genetically Modified, RNA Interference, RNA, Small Interfering, Cytochrome P-450 Enzyme Inhibitors

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Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol

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