Cited by 1,358Insect Resistance and GeneticsInsect-Plant Interactions and ControlInsect Pest Control Strategies

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Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol

Mao, Ying-Bo, Cai, Wen-Juan, Wang, Jia-Wei, Hong, Gao-Jie, Tao, Xiao-Yuan, Wang, Ling-Jian, Huang, Yong-Ping, Chen, Xiao-Ya·2007·Nature Biotechnology

DOI 10.1038/nbt1352

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Abstract

We identify a cytochrome P450 gene (CYP6AE14) from cotton bollworm (Helicoverpa armigera), which permits this herbivore to tolerate otherwise inhibitory concentrations of the cotton metabolite, gossypol. CYP6AE14 is highly expressed in the midgut and its expression correlates with larval growth when gossypol is included in the diet. When larvae are fed plant material expressing double-stranded RNA (dsRNA) specific to CYP6AE14, levels of this transcript in the midgut decrease and larval growth is retarded. Both effects are more dramatic in the presence of gossypol. As a glutathione-S-transferase gene (GST1) is silenced in GST1 dsRNA-expressing plants, feeding insects plant material expressing dsRNA may be a general strategy to trigger RNA interference and could find applications in entomological research and field control of insect pests.

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Clough, S. J., & Bent, A. F. (1998) · The Plant Journal · doi:10.1046/j.1365-313x.1998.00343.x · Cited by 22,014
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
Specific interference by ingested dsRNA
Timmons, L., & Fire, A. Z. (1998) · Nature · doi:10.1038/27579 · Cited by 1,938
Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
Timmons, L., Court, D. L., & Fire, A. Z. (2001) · Gene · doi:10.1016/s0378-1119(00)00579-5 · Cited by 1,840
Genetic and Functional Diversification of Small RNA Pathways in Plants
Xie, Z., Johansen, L. K., Gustafson, A. M., Kasschau, K. D., Lellis, A. D., Zilberman, D., Jacobsen, S. E., & Carrington, J. C. (2004) · PLoS Biology · doi:10.1371/journal.pbio.0020104 · Cited by 1,569
Construct design for efficient, effective and high‐throughput gene silencing in plants
Wesley, S. V., Helliwell, C. A., Smith, N. A., Wang, M., Rouse, D. T., Liu, Q., Gooding, P. S., Singh, S. P., Abbott, D., Stoutjesdijk, P. A., et al. (2001) · The Plant Journal · doi:10.1046/j.1365-313x.2001.01105.x · Cited by 1,542
The rde-1 Gene, RNA Interference, and Transposon Silencing in C. elegans
Tabara, H., Sarkissian, M., Kelly, W. G., Fleenor, J., Grishok, A., Timmons, L., Fire, A. Z., & Mello, C. C. (1999) · Cell · doi:10.1016/s0092-8674(00)81644-x · Cited by 1,307
Superoxide radical inhibits catalase.
Kono, Y., & Fridovich, I. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)83842-5 · Cited by 1,254
Systemic RNAi in C. elegans Requires the Putative Transmembrane Protein SID-1
Winston, W. M., Molodowitch, C., & Hunter, C. P. (2002) · Science · doi:10.1126/science.1068836 · Cited by 849
Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline
Aravin, A. A., Nm, N., Tulin, A. V., Vagin, V. V., Rozovsky, Y. M., & Gvozdev, V. A. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00299-8 · Cited by 827

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Yang, C., Preisser, E. L., Zhang, H., Liu, Y., Dai, L., Pan, H., & Zhou, X. J. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.01672 · Cited by 40
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Co-silence of the coatomer β and v-ATPase A genes by siRNA feeding reduces larval survival rate and weight gain of cotton bollworm, Helicoverpa armigera
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Cathepsin B Cysteine Proteinase is Essential for the Development and Pathogenesis of the Plant Parasitic Nematode Radopholus similis
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“It has also been used to study the control methods of many pests including the Rice stripe virus [22], Rice tungro bacilliform virus [23], Diabrotica virgifera virgifera [24], Myzus persicae [25, 26], Nilaparvata lugens [27], Helicoverpa armigera and Spodoptera exigua [28, 29] and some sedentary plant parasitic nemato…”
Method
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Characterization and Comparative Analysis of Small RNAs in Three Small RNA Libraries of the Brown Planthopper (Nilaparvata lugens)
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“As there is increasingly more research on small RNAs,the use of small RNA was also increased, especially in the field of RNA interference (RNAi) [1,2,3,4].”
Background
Cotton roots are the major source of gossypol biosynthesis and accumulation
Zhao, T., Xie, Q., Li, C., et al. (2020) · BMC Plant Biology · doi:10.1186/s12870-020-2294-9 · Cited by 39
Transgene-Induced Gene Silencing in Plants
Jin, Y. H., & Guo, H. (2015) · Methods in molecular biology · doi:10.1007/978-1-4939-2453-0_7 · Cited by 39
A dsRNA delivery system based on the rosin‐modified polyethylene glycol and chitosan induces gene silencing and mortality in Nilaparvata lugens
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Lepidopteran insect species‐specific, broad‐spectrum, and systemic RNA interference by spraying ds RNA on larvae
Zhang, H., Li, H., Guan, R., et al. (2015) · Entomologia Experimentalis et Applicata · doi:10.1111/eea.12300 · Cited by 38
“This kind of dsRNA uptake mechanism can be applied to transgenic plants for crop protection (Baum et al., 2007; Mao et al., 2007, 2011).”
Background
Fork head transcription factor is required for ovarian mature in the brown planthopper, Nilaparvata lugens (Stål)
Dong, X., Zhai, Y., Zhang, J., et al. (2011) · BMC Molecular Biology · doi:10.1186/1471-2199-12-53 · Cited by 38
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RNA Interference in the Tobacco Hornworm, Manduca sexta, Using Plastid-Encoded Long Double-Stranded RNA
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“In fact, gene knockout of RNAi processing pathway components within nuclear dsRNA-expressing plants increases the ability of these plants to resist feeding by cotton bollworm (Mao et al., 2007), a result which suggests that the plant’s intrinsic ability to process dsRNA into siRNA is responsible for the reduced RNAi e…”
Background
Functionally characterized arthropod pest and pollinator cytochrome P450s associated with xenobiotic metabolism
Katsavou, E., Riga, M., Ioannidis, P., et al. (2021) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2021.105005 · Cited by 37
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De novo Cloning and Annotation of Genes Associated with Immunity, Detoxification and Energy Metabolism from the Fat Body of the Oriental Fruit Fly, Bactrocera dorsalis
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“In recent years, RNA interference (RNAi)-based technology has shown great potential in pest control by silencing vital genes [3,4].”
Background
RNA interference-mediated knockdown of a cytochrome P450 gene enhanced the toxicity of α-cypermethrin in xanthotoxin-fed larvae of Spodoptera exigua (Hübner)
Hafeez, M., Liu, S., Yousaf, H. K., et al. (2019) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2019.07.003 · Cited by 37
“against xenobiotics such as insecticides [12,58].”
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Zhang, B., Su, X., Xie, L., Zhen, C., Hu, G.-L., Jiang, K., Huang, Z., Liu, R., Gao, Y.-F., Chen, X., et al. (2019) · Crop Protection · doi:10.1016/j.cropro.2019.105014 · Cited by 41
Silencing arginine kinase/integrin β1subunit by transgenic plant expressing dsRNA inhibits the development and survival of Plutella xylostella
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Analysis of RNA Interference (RNAi) Biopesticides: Double-Stranded RNA (dsRNA) Extraction from Agricultural Soils and Quantification by RT-qPCR
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Zhao, J., Yan, S., Li, M., Sun, L., Dong, M., Yin, M., Shen, J., & Zhao, Z. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.125816 · Cited by 36
Endocytosis-mediated entry of a caterpillar effector into plants is countered by Jasmonate
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“However, despite many years of research there is no consensus, which genes or pathways are the best targets for RNAi-mediated pest control.”
Recent insights into the molecular mechanism of jasmonate signaling during insect-plant interaction
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“…induce a broad spectrum of defensive responses that include antioxidative enzymes, PIs, alkaloid production and trichome formation (Dickens 2006; Mao et al. 2007; Pauwels et al. 2009) and result in a significant improvement of the plant resistance to herbivore damage (Arimura et al. 2009; War…”
Background
Functional expression of a peritrophin A-like SfPER protein is required for larval development in Spodoptera frugiperda (Lepidoptera: Noctuidae)
Noval, C., Rodríguez–Cabrera, L., Izquierdo, L., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-38734-0 · Cited by 35
Contemporary evolution of a Lepidopteran species, Heliothis virescens , in response to modern agricultural practices
Fritz, M. L., DeYonke, A. M., Papanicolaou, A., Micinski, S., Westbrook, J. K., & Gould, F. L. (2017) · Molecular Ecology · doi:10.1111/mec.14430 · Cited by 35
“Indeed, RNAi silencing of Cyp6AE14 in H. armigera led to a decline in larval growth when gossypol was present in their diet (Mao et al. 2007).”
BackgroundInfluential
Transgenic rice overexpressing insect endogenous microRNA csu‐novel‐260 is resistant to striped stem borer under field conditions
Zheng, X., Weng, Z., Li, H., et al. (2020) · Plant Biotechnology Journal · doi:10.1111/pbi.13504 · Cited by 35
“In the first two proof-of-concept studies, DNA fragments derived from essential insect genes were constructed into plant expression cassettes as inverted repeats, enabling long double-stranded RNAs (dsRNAs) to be transcribed in host plants (Baum et al., 2007; Mao et al., 2007).”
Background
Disruption of the cytochrome P450 CYP6BQ7 gene reduces tolerance to plant toxicants in the red flour beetle, Tribolium castaneum
Zhang, Y.-C., Gao, S., Xue, S., An, S., & Zhang, K. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.01.054 · Cited by 35
Genetic Engineering Approaches for Enhanced Insect Pest Resistance in Sugarcane
Iqbal, A., Khan, R. S., Khan, M. A., Gul, K., Jalil, F., Shah, D. A., Rahman, H., & Ahmed, T. A.-F. (2021) · Molecular Biotechnology · doi:10.1007/s12033-021-00328-5 · Cited by 36
RNAi Targets in Agricultural Pest Insects: Advancements, Knowledge Gaps, and IPM
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“In 2007, two studies demonstrated potential for double-stranded RNA (dsRNA) to act as a nucleotide sequence-specific insecticide, as it induced RNA interference (RNAi) in insects consuming dsRNA comprising an inverted repeat of a target gene’s coding sequence (Baum et al., 2007; Mao et al., 2007).”
Background
Successful Silencing of the Mycotoxin Synthesis Gene TRI5 in Fusarium culmorum and Observation of Reduced Virulence in VIGS and SIGS Experiments
Tretiakova, P. Y., Voegele, R. T., Soloviev, A., et al. (2022) · Genes · doi:10.3390/genes13030395 · Cited by 35
“It has been used on plants affected by nematodes [13], insects [14,15], and fungi [16–19].”
Method
Insect Detoxification and Sequestration Strategies
Heckel, D. G. (2017) · Annual Plant Reviews online · doi:10.1002/9781119312994.apr0507 · Cited by 35
Development and Adoption of Genetically Engineered Plants for Virus Resistance: Advances, Opportunities and Challenges
Niraula, P. M., & Fondong, V. N. (2021) · Plants · doi:10.3390/plants10112339 · Cited by 34
“Other gene silencing approaches have developed virus resistance in insect vectors in order to limit virus transmission [32].”
Background
Chemically modified dsRNA induces RNAi effects in insects in vitro and in vivo: A potential new tool for improving RNA-based plant protection
Howard, J. D., Beghyn, M., Dewulf, N., Vos, Y. D., Philips, A., Portwood, D. E., Kilby, P. M., Oliver, D., Maddelein, W., Brown, S., et al. (2022) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2022.102311 · Cited by 37
“The first successful studies demonstrating proof of principle for this approach in insects took place 15 years ago (10, 11), and in the intervening years research has been undertaken demonstrating the possibility of using this approach for a wide range of targets and insect species (15–20).”
Background
In vitro and in vivo application of RNA interference for targeting genes involved in peritrophic matrix synthesis in a lepidopteran system
Toprak, U., Baldwin, D., Erlandson, M. A., et al. (2012) · Insect Science · doi:10.1111/j.1744-7917.2012.01562.x · Cited by 34
“This technique also has the potential to be part of new insect control strategies (Asokan, 2008) and the use of transgenic plants expressing dsRNA for target genes is promising (Baum et al., 2007; Mao et al., 2007).”
Background
Biotechnologically generating ‘super chickpea’ for food and nutritional security
Acharjee, S., & Sarmah, B. K. (2013) · Plant Science · doi:10.1016/j.plantsci.2013.02.003 · Cited by 35
RNA Interference Technology to Control Pest Sea Lampreys - A Proof-of-Concept
Heath, G., Childs, D., Docker, M. F., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0088387 · Cited by 34
“Two research groups demonstrated that plants could be genetically engineered to express insect-specific dsRNAs that would kill the insects that feed on them [22],[23].”
Background
Increased virulence in the locust‐specific fungal pathogen Metarhizium acridum expressing dsRNAs targeting the host F1F0‐ATPase subunit genes
Hu, J., & Xia, Y. (2018) · Pest Management Science · doi:10.1002/ps.5085 · Cited by 34
RNA interference in Colorado potato beetle (Leptinotarsa decemlineata): A potential strategy for pest control
Ma, M., He, W., Xu, S., Xu, L., & Zhang, J. (2020) · Journal of Integrative Agriculture · doi:10.1016/s2095-3119(19)62702-4 · Cited by 36
Silencing of multiple target genes via ingestion of dsRNA and PMRi affects development and survival in Helicoverpa armigera
Sharif, M. N., Iqbal, M. S., Alam, R., Awan, M. F., Tariq, M., Ali, Q., & Nasir, I. A. (2022) · Scientific Reports · doi:10.1038/s41598-022-14667-z · Cited by 34
Systemic RNA interference in locusts: reverse genetics and possibilities for locust pest control
Santos, D., Broeck, J. V., & Wynant, N. (2014) · Current Opinion in Insect Science · doi:10.1016/j.cois.2014.09.013 · Cited by 34
Plant disease management in India: Advances and challenges
Sanjeev, K. (2014) · African Journal of Agricultural Research · doi:10.5897/ajar2014.7311 · Cited by 34
Analysis of ESTs generated from immune-stimulated hemocytes of larval Heliothis virescens
Shelby, K. S., & Popham, H. J. R. (2009) · Journal of Invertebrate Pathology · doi:10.1016/j.jip.2009.05.002 · Cited by 33
RNA interference in shrimp and potential applications in aquaculture
Dũng, N. V., Christiaens, O., Bossier, P., et al. (2016) · Reviews in Aquaculture · doi:10.1111/raq.12187 · Cited by 33
“Finally, oral delivery of dsRNA, either in the form of naked dsRNA or in the form of bacteria that contain the specific dsRNA, has been successful in many arthropod species as well (Baum et al. 2007; Mao et al. 2007; Whyard et al. 2009; Wuriyanghan et al. 2011).”
Background
Plant training for induced defense against insect pests: a promising tool for integrated pest management in cotton
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Transgenic plants expressing the AaIT/GNA fusion protein show increased resistance and toxicity to both chewing and sucking pests
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“There have been reports of arming plants with double-stranded RNA (dsRNA) to protect against insect pests, as transgenic plants producing dsRNAs targeting selected insect genes can suppress insect growth and development (Baum et al., 2007; Mao et al., 2007; Zhu et al., 2012; Katoch & Thakur, 2013).”
Background
Detoxification enzymes of Bemisia tabaci B and Q: biochemical characteristics and gene expression profiles
Guo, L., Xie, W., Wang, S., et al. (2014) · Pest Management Science · doi:10.1002/ps.3751 · Cited by 33
“Lower expression of COE2 in Q than B was previously reported by Kang et al. (2012). Elbaz et al.”
Result
Do we have the tools to manage resistance in the future?
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Beernink, B. M., Amanat, N., Li, V. H., Manchur, C. L., Whyard, S., & Belmonte, M. F. (2024) · Canadian Journal of Plant Pathology · doi:10.1080/07060661.2024.2392610 · Cited by 38
“HIGS-based technologies have been developed to protect crop plants from viruses (Abel et al. 1986), nematodes (Huang et al. 2006), insects (Baum et al. 2007; Mao et al. 2007), fungi (M. Wang et al. 2016; Koch et al. 2016; McLoughlin et al. 2018b) and oomycetes (Vega-Arreguín et al. 2014).”
Crop protection by RNA interference: a review of recent approaches, current state of developments and use as of 2013
Germing, K., Navarrete, C. A. D., Schiermeyer, A., Hommen, U., Zühl, L., Eilebrecht, S., & Eilebrecht, E. (2025) · Environmental Sciences Europe · doi:10.1186/s12302-025-01052-6 · Cited by 32
“Only a few years after its discovery, the RNAi technique was first shown to be effective for targeting pest organisms like plant parasitic nematodes [91] and for other applications in crop protection [4, 60].”
Targeting insect mitochondrial complex I for plant protection
Wu, X., Yang, C., Mao, Y., Wang, L., Shangguan, X., & Chen, X. (2016) · Plant Biotechnology Journal · doi:10.1111/pbi.12553 · Cited by 32
“Plant-mediated RNA interference (PM-RNAi) engineers plant to express double-stranded RNA (dsRNA) targeting to herbivorous the insect gene and suppress its expression after ingestion (Baum et al., 2007; Mao et al., 2007).”
BackgroundMethodInfluential
Identification of microRNAs and their response to the stress of plant allelochemicals in Aphis gossypii (Hemiptera: Aphididae)
Ma, K., Li, F., Liu, Y., et al. (2017) · BMC Molecular Biology · doi:10.1186/s12867-017-0080-5 · Cited by 32
“armigera larvae were exposed to a high concentration of gossypol [33, 34].”
Background
Host plant driven transcriptome plasticity in the salivary glands of the cabbage looper (Trichoplusia ni)
Rivera-Vega, L. J., Galbraith, D. A., Grozinger, C. M., et al. (2017) · PLoS ONE · doi:10.1371/journal.pone.0182636 · Cited by 32
Transcription Factors AhR/ARNT Regulate the Expression of CYP6CY3 and CYP6CY4 Switch Conferring Nicotine Adaptation
Pan, Y., Peng, T., Xu, P., Zeng, X., Tian, F., Song, J., & Shang, Q. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20184521 · Cited by 31
“Larval tolerance to gossypol in the cotton bollworm was reduced following CYP6AE14 silencing with plant-mediated RNAi [29,30].”
Background
Transcriptome analysis of putative detoxification genes in the Asian citrus psyllid, Diaphorina citri
Wu, Z., Pu, X., Shu, B., et al. (2020) · Pest Management Science · doi:10.1002/ps.5937 · Cited by 32
Biotic stress triggered small RNA and RNAi defense response in plants
Ali, M., Javaid, A., Naqvi, S. H., Batcho, A., Kayani, W. K., Lal, A., Sajid, I. A., & Nwogwugwu, J. O. (2020) · Molecular Biology Reports · doi:10.1007/s11033-020-05583-4 · Cited by 32
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Noman, A., Aqeel, M. A., Islam, W., Khalid, N., Akhtar, N., Qasim, M. U., Yasin, G., Hashem, M., Alamri, S., Zoubi, O. M. A., et al. (2021) · Toxicon · doi:10.1016/j.toxicon.2021.04.004 · Cited by 33
Host induced gene silencing of Magnaporthe oryzae by targeting pathogenicity and development genes to control rice blast disease
Wang, M., & Dean, R. A. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.959641 · Cited by 34
“This concept was successful for control of rootknot nematodes and of lepidopteran and coleopteran insects feeding on transgenic plants carrying RNAi constructs targeting corresponding pest genes (Huang et al., 2006; Baum et al., 2007; Mao et al., 2007).”
Background
FAR knockout significantly inhibits Chilo suppressalis survival and transgene expression of double‐stranded FAR in rice exhibits strong pest resistance
Sun, Y., Gong, Y., He, Q., et al. (2022) · Plant Biotechnology Journal · doi:10.1111/pbi.13906 · Cited by 31
“Plant-mediated RNA interference (RNAi) is a post-transcriptional double-stranded RNA (dsRNA)-mediated gene silencing mechanism that has demonstrated excellent potential as an effective and eco-friendly alternative method of insect pest control (Aravin et al., 2001; Baum et al., 2007; Chung et al., 2021; Mao et al., 20…”
Method
Integrated resistance management for acaricide use on Varroa destructor
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Double-Stranded RNAs High-Efficiently Protect Transgenic Potato from Leptinotarsa decemlineata by Disrupting Juvenile Hormone Biosynthesis
Guo, W., Bai, C., Wang, Z., Wang, P., Fan, Q., Mi, X., Wang, L., He, J., Pang, J., Luo, X., et al. (2018) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.8b03914 · Cited by 31
RNA interference mediated knockdown of juvenile hormone esterase gene in Bemisia tabaci (Gennadius): Effects on adults and their progeny
Grover, S., Jindal, V., & Banta, G. (2018) · Journal of Asia-Pacific Entomology · doi:10.1016/j.aspen.2018.12.002 · Cited by 31
The green peach aphid gut contains host plant microRNAs identified by comprehensive annotation of Brassica oleracea small RNA data
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Candidate genetic determinants of intraspecific variation in pea aphid susceptibility to RNA interference
Yoon, J., Tian, H., McMullen, J. G., Chung, S. H., & Douglas, A. E. (2020) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2020.103408 · Cited by 31
Knockdown of the Trehalose-6-Phosphate Synthase Gene Using RNA Interference Inhibits Synthesis of Trehalose and Increases Lethality Rate in Asian Citrus Psyllid, Diaphorina citri (Hemiptera: Psyllidae)
Liu, X., Zou, Z., Zhang, C., et al. (2020) · Insects · doi:10.3390/insects11090605 · Cited by 31
Double-stranded RNA in exosomes: Potential systemic RNA interference pathway in the Colorado potato beetle, Leptinotarsa decemlineata
Yoon, J., Kim, K., & Palli, S. R. (2020) · Journal of Asia-Pacific Entomology · doi:10.1016/j.aspen.2020.09.012 · Cited by 31
Roles of the variable P450 substrate recognition sites SRS1 and SRS6 in esfenvalerate metabolism by CYP6AE subfamily enzymes in Helicoverpa armigera
Shi, Y., O’Reilly, A. O., Sun, S., et al. (2020) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2020.103486 · Cited by 31
Chromosome‐level genome reference and genome editing of the tea geometrid
Pan, Y., Fang, G., Wang, Z., et al. (2021) · Molecular Ecology Resources · doi:10.1111/1755-0998.13385 · Cited by 31
Identification and characteristics of a novel gland‐forming gene in cotton
Zang, Y., Xu, C., Xuan, L., et al. (2021) · The Plant Journal · doi:10.1111/tpj.15477 · Cited by 31
Peritrophic Matrices
Merzendorfer, H., Kelkenberg, M., & Muthukrishnan, S. (2016) · Springer eBooks · doi:10.1007/978-3-319-40740-1_8 · Cited by 30
Resistance to RNA interference by plant‐derived double‐stranded RNAs but not plant‐derived short interfering RNAs in Helicoverpa armigera
Fu, J., Xu, S., Lu, H., Li, F., Li, S., Chang, L., Heckel, D. G., Bock, R., & Zhang, J. (2022) · Plant Cell & Environment · doi:10.1111/pce.14314 · Cited by 31
Tribolium castaneum as a Model for High-Throughput RNAi Screening
Knorr, E., Bingsohn, L., Kanost, M. R., & Vilcinskas, A. (2013) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2013_208 · Cited by 32
Synergistic Interactions Between Cry1Ac and Natural Cotton Defenses Limit Survival of Cry1Ac-resistant Helicoverpa Zea (Lepidoptera: Noctuidae) on Bt Cotton
Anilkumar, K. J., Sivasupramaniam, S., Head, G., et al. (2009) · Journal of Chemical Ecology · doi:10.1007/s10886-009-9665-x · Cited by 31
“explanation for synergistic effects of gossypol and Cry1Ac only on AR-larvae is that gossypol is degraded by upregulation of a cytochrome P450 in Helicoverpa armigera (Mao et al. 2007).”
Background
Identification of differential expression genes associated with host selection and adaptation between two sibling insect species by transcriptional profile analysis
Li, H., Zhang, H., Guan, R., et al. (2013) · BMC Genomics · doi:10.1186/1471-2164-14-582 · Cited by 31
RNA interference-based pesticides: Mechanism, application, and commercialization in sustainable pest management
Li, Z., Liu, Y., Liang, Y., Pan, T., & Liu, J. (2026) · Pesticide Biochemistry and Physiology · doi:10.1016/j.pestbp.2026.107034 · Cited by 32
Host-Induced Gene Silencing of an Adenylate Kinase Gene Involved in Fungal Energy Metabolism Improves Plant Resistance to Verticillium dahliae
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Host-induced gene silencing of brown planthopper glutathione S-transferase gene enhances rice resistance to sap-sucking insect pests
Yang, J., Sun, X., Zhu‐Salzman, K., Qin, Q., Feng, H., Kong, X., Zhou, X. J., & Cai, Q. (2020) · Journal of Pest Science · doi:10.1007/s10340-020-01296-6 · Cited by 30
Krüppel homolog 1 regulates photoperiodic reproductive plasticity in the cabbage beetle Colaphellus bowringi
Guo, S., Wu, Q., Tian, Z., Zhu, L., King‐Jones, K., Zhu, F., Wang, X., & Liu, W. (2021) · Insect Biochemistry and Molecular Biology · doi:10.1016/j.ibmb.2021.103582 · Cited by 31
Activator protein‐1 mediated CYP6ER1 overexpression in the clothianidin resistance of Nilaparvata lugens (Stål)
Jin, R., Wang, Y., He, B., Zhang, Y., Cai, T., Wan, H., Jin, B. R., & Li, J. (2021) · Pest Management Science · doi:10.1002/ps.6482 · Cited by 28
Vacuolar ATPase subunit H is essential for the survival and moulting of Locusta migratoria manilensis
Li, C., & Xia, Y. (2012) · Insect Molecular Biology · doi:10.1111/j.1365-2583.2012.01147.x · Cited by 30
“Additionally, gene silencing by RNAi in insect pests has been successfully applied in the development of transgenic maize (Baum et al., 2007) and cotton (Mao et al., 2007).”
Background
Genome editing in cotton: challenges and opportunities
Khan, Z., Khan, S. H., Ahmed, A., Iqbal, M. U., Mubarik, M. S., Ghouri, M. Z., Ahmad, F., Yaseen, S., Ali, Z., Khan, A. A., et al. (2023) · Journal of Cotton Research · doi:10.1186/s42397-023-00140-3 · Cited by 35
“The 12 and 13-positioned amino acids in one TALE monomer are responsible for the specificity of the TALE monomer to the DNA base (Mao et al. 2007).”
Background
Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions
Chaudhary, D., Jeena, A. S., Rohit, R., Gaur, S., Raj, R., Mishra, S., Kajal, Gupta, O. P., & Meena, M. R. (2024) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-023-04850-x · Cited by 32
A Comparison of Physical, Chemical, Biological and Combined Treatments for Detoxification of Free Gossypol in Crushed Whole Cottonseed
Neto, C. B. S., Conceição, A. A., Gomes, T. G., et al. (2020) · Waste and Biomass Valorization · doi:10.1007/s12649-020-01290-0 · Cited by 29
Emerald Ash Borer Specific Gene Silencing Has No Effect on Non-target Organisms
Pampolini, F., & Rieske, L. K. (2020) · Frontiers in Agronomy · doi:10.3389/fagro.2020.608827 · Cited by 29
“, 2016), or expressed in transgenic plants (Baum et al., 2007; Mao et al., 2007; Ghag, 2017; Zhang et al., 2017).”
Background
Identification and functional analysis of dsRNases in spotted‐wing drosophila, Drosophila suzukii
Yoon, J., Ahn, S., Flinn, C. M., et al. (2021) · Archives of Insect Biochemistry and Physiology · doi:10.1002/arch.21822 · Cited by 29
Crataegus oxyacantha Extract as a Biostimulant to Enhance Tolerance to Salinity in Tomato Plants
Naboulsi, I., Mrid, R. B., Ennoury, A., et al. (2022) · Plants · doi:10.3390/plants11101283 · Cited by 29
“Chlorophyll content in plants is the main factor that reflects their photosynthetic rate [21].”
Background
Small RNAs: Efficient and miraculous effectors that play key roles in plant–microbe interactions
Jiang, C., Li, Z., Zheng, L., et al. (2023) · Molecular Plant Pathology · doi:10.1111/mpp.13329 · Cited by 29
Host-Delivered RNA Interference for Durable Pest Resistance in Plants: Advanced Methods, Challenges, and Applications
Saakre, M., Jaiswal, S., Rathinam, M., Raman, K. V., Tilgam, J., Paul, K., Sreevathsa, R., & Pattanayak, D. (2023) · Molecular Biotechnology · doi:10.1007/s12033-023-00833-9 · Cited by 30
“Further, pRNAi-GG is a binary vector that avoids two-step cloning (ligation of DNA fragment into cloning vectors such as pUC19 and then to binary vector) and can be directly used for plant transformation [40, 76].”
Expression and RNA Interference of Salivary Polygalacturonase Genes in the Tarnished Plant Bug,Lygus lineolaris
Walker, W. B., & Allen, M. L. (2010) · Journal of Insect Science · doi:10.1673/031.010.14133 · Cited by 29
Particle Bombardment of the cry2A Gene Cassette Induces Stem Borer Resistance in Sugarcane
Gao, S., Yang, Y., Xu, L., et al. (2018) · International Journal of Molecular Sciences · doi:10.3390/ijms19061692 · Cited by 29
Insect omics research coming of age1This review is part of a virtual symposium on recent advances in understanding a variety of complex regulatory processes in insect physiology and endocrinology, including development, metabolism, cold hardiness, food intake and digestion, and diuresis, through the use of omics technologies in the postgenomic era.
Boerjan, B., Cardoen, D., Verdonck, R., Caers, J., & Schoofs, L. (2012) · Canadian Journal of Zoology · doi:10.1139/z2012-010 · Cited by 29
Identification and Characterization of the Gene CYP340W1 from Plutella xylostella and Its Possible Involvement in Resistance to Abamectin
Gao, X., Yang, J., Xu, B., et al. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17030274 · Cited by 28
“Unlike other P450 genes that are highly expressed in the midgut [33,34], which is regarded as the primary detoxification organ, CYP340W1 is much more abundant in the head than in other tissues.”
Background

Authors: Mao, Y.-B., Cai, W.-J., Wang, J.-W., Hong, G.-J., Tao, X.-Y., Wang, L.-J., Huang, Y.-P., & Chen, X.-Y.
Affiliations: Chinese Academy of Sciences (CN)
Corresponding author: Chen, Xiao-Ya
ORCID: Mao, Ying-Bo
Wang, Ling-Jian
Chen, Xiao-Ya
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2007-11
Volume: 25
Issue: 11
Pages: 1307-1313
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt1352
OpenAlex: W2121440246
Cited by: 1,358
References: 50
Citations by year: 2026: 44 · 2025: 76 · 2024: 80 · 2023: 56 · 2022: 111 · 2021: 98 · 2020: 84 · 2019: 87 · 2018: 67 · 2017: 95 · 2016: 92 · 2015: 89 · 2014: 84 · 2013: 77 · 2012: 68
FWCI: 13.10
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
Keywords: Helicoverpa armigera, cytochrome P450 monooxygenase, dsRNA feeding, midgut gene expression, larval growth, insect pest control, plant-insect interactions
MeSH terms: Animals, Gossypium, Cytochrome P-450 Enzyme System, Digestion, Drug Resistance, Glutathione Transferase, Gossypol, Larva, Lepidoptera, Molecular Sequence Data, Pest Control, Biological, RNA, Double-Stranded, Nicotiana, Arabidopsis, RNA, Plant, Insect Proteins, Gene Silencing, Plants, Genetically Modified, RNA Interference, RNA, Small Interfering, Cytochrome P-450 Enzyme Inhibitors

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Silencing a cotton bollworm P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol

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