The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N
Open accessAbstract
Centromeres are specialized chromosomal domains that direct kinetochore assembly during mitosis. CENP-A (centromere protein A), a histone H3-variant present exclusively in centromeric nucleosomes, is thought to function as an epigenetic mark that specifies centromere identity. Here we identify the essential centromere protein CENP-N as the first protein to selectively bind CENP-A nucleosomes but not H3 nucleosomes. CENP-N bound CENP-A nucleosomes in a DNA sequence-independent manner, but did not bind soluble CENP-A–H4 tetramers. Mutations in CENP-N that reduced its affinity for CENP-A nucleosomes caused defects in CENP-N localization and had dominant effects on the recruitment of CENP-H, CENP-I and CENP-K to centromeres. Depletion of CENP-N using siRNA (short interfering RNA) led to similar centromere assembly defects and resulted in reduced assembly of nascent CENP-A into centromeric chromatin. These data suggest that CENP-N interprets the information encoded within CENP-A nucleosomes and recruits other proteins to centromeric chromatin that are required for centromere function and propagation.
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Cites
Molecular architecture of the kinetochore–microtubule interface
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The human CENP-A centromeric nucleosome-associated complex
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Self-citationMasumoto, H., Masukata, H., Muro, Y., Nozaki, N., & Okazaki, T. (1989) · The Journal of cell biology · doi:10.1083/jcb.109.5.1963 · Cited by 685
Propagation of centromeric chromatin requires exit from mitosis
Jansen, L. E. T., Black, B. E., Foltz, D. R., & Cleveland, D. W. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200701066 · Cited by 629
Self-citationOkada, M., Cheeseman, I. M., Hori, T., Okawa, K., McLeod, I. X., Yates, J. R., Desai, A., & Fukagawa, T. (2006) · Nature Cell Biology · doi:10.1038/ncb1396 · Cited by 509
Mis16 and Mis18 Are Required for CENP-A Loading and Histone Deacetylation at Centromeres
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Structural determinants for generating centromeric chromatin
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Priming of Centromere for CENP-A Recruitment by Human hMis18α, hMis18β, and M18BP1
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Cited by
The molecular basis for centromere identity and function
McKinley, K. L., & Cheeseman, I. M. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2015.5 · Cited by 668
A Molecular View of Kinetochore Assembly and Function
Musacchio, A., & Desai, A. (2017) · Biology · doi:10.3390/biology6010005 · Cited by 544
Induced Ectopic Kinetochore Assembly Bypasses the Requirement for CENP-A Nucleosomes
Gascoigne, K. E., Takeuchi, K., Suzuki, A., Hori, T., Fukagawa, T., & Cheeseman, I. M. (2011) · Cell · doi:10.1016/j.cell.2011.03.031 · Cited by 332
HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore
Barnhart, M. C., Kuich, P. H. J. L., Stellfox, M. E., Ward, J. A., Bassett, E. A., Black, B. E., & Foltz, D. R. (2011) · Journal of Cell Biology · doi:10.1083/jcb.201012017 · Cited by 329
A Conserved Mechanism for Centromeric Nucleosome Recognition by Centromere Protein CENP-C
Kato, H., Jiang, J., Zhou, B.-R., Rozendaal, M., Feng, H., Ghirlando, R., Xiao, T. S., Straight, A. F., & Bai, Y. (2013) · Science · doi:10.1126/science.1235532 · Cited by 323
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Metadata
- Authors
- Carroll, C. W., Silva, M. C. C., Godek, K. M., Jansen, L. E. T., & Straight, A. F.
- Venue
- Nature Cell Biology
- Published
- 2009-06-21
- Volume
- 11
- Issue
- 7
- Pages
- 896-902
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2018707048
- Cited by
- 291
- References
- 22
- FWCI
- 9.61
- Type
- journal article
- Language
- en
- Open access
- green
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