The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
GAPDH mediates nitrosylation of nuclear proteins
Open accessAbstract
S-nitrosylation of proteins by nitric oxide is a major mode of signalling in cells. S-nitrosylation can mediate the regulation of a range of proteins, including prominent nuclear proteins, such as HDAC2 (ref. 2) and PARP1 (ref. 3). The high reactivity of the nitric oxide group with protein thiols, but the selective nature of nitrosylation within the cell, implies the existence of targeting mechanisms. Specificity of nitric oxide signalling is often achieved by the binding of nitric oxide synthase (NOS) to target proteins, either directly or through scaffolding proteins such as PSD-95 (ref. 5) and CAPON. As the three principal isoforms of NOS—neuronal NOS (nNOS), endothelial NOS (eNOS) and inducible NOS (iNOS) —are primarily non-nuclear, the mechanisms by which nuclear proteins are selectively nitrosylated have been elusive. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is physiologically nitrosylated at its Cys 150 residue. Nitrosylated GAPDH (SNO–GAPDH) binds to Siah1, which possesses a nuclear localization signal, and is transported to the nucleus. Here, we show that SNO–GAPDH physiologically transnitrosylates nuclear proteins, including the deacetylating enzyme sirtuin-1 (SIRT1), histone deacetylase-2 (HDAC2) and DNA-activated protein kinase (DNA-PK). Our findings reveal a novel mechanism for targeted nitrosylation of nuclear proteins and suggest that protein–protein transfer of nitric oxide groups may be a general mechanism in cellular signal transduction.
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Cites
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Cited by
Kauppinen, A., Suuronen, T., Ojala, J., Kaarniranta, K., & Salminen, A. (2013) · Cellular Signalling · doi:10.1016/j.cellsig.2013.06.007 · Cited by 989
Redox regulation of SIRT1 in inflammation and cellular senescence
Hwang, J.-W., Yao, H., Caito, S., Sundar, I. K., & Rahman, I. (2013) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2013.03.015 · Cited by 503
Zhong, Q., Xiao, X., Qiu, Y., Xu, Z., Chen, C., Chong, B., Zhao, X., Hai, S., Li, S., An, Z., et al. (2023) · MedComm · doi:10.1002/mco2.261 · Cited by 442
Aberrant Protein S-Nitrosylation in Neurodegenerative Diseases
Nakamura, T., Tu, S., Akhtar, M. W., Sunico, C. R., Okamoto, S.-I., & Lipton, S. A. (2013) · Neuron · doi:10.1016/j.neuron.2013.05.005 · Cited by 355
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Metadata
- Authors
- Kornberg, M. D., Sen, N., Hara, M. R., Juluri, K. R., Nguyen, J. V. K., Snowman, A. M., Law, L., Hester, L. D., & Snyder, S. H.
- Venue
- Nature Cell Biology
- Published
- 2010-10-24
- Volume
- 12
- Issue
- 11
- Pages
- 1094-1100
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W1977535645
- Cited by
- 394
- References
- 9
- FWCI
- 21.69
- Type
- journal article
- Language
- en
- Open access
- green
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- Kornberg, M. D., Sen, N., Hara, M. R., Juluri, K. R., Nguyen, J. V. K., Snowman, A. M., Law, L., Hester, L. D., & Snyder, S. H. (2010). GAPDH mediates nitrosylation of nuclear proteins. Nature Cell Biology. https://doi.org/10.1038/ncb2114
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