Proteomic and genomic approaches reveal critical functions of H3K9 methylation and heterochromatin protein-1γ in reprogramming to pluripotency

Sridharan, Rupa; Gonzales-Cope, Michelle; Chronis, Constantinos; Bonora, Giancarlo; McKee, Robin; Huang, Chengyang; Patel, Sanjeet; Lopez, David; Mishra, Nilamadhab; Pellegrini, Matteo; Carey, Michael; Garcia, Benjamin A.; Plath, Kathrin

doi:10.1038/ncb2768

Proteomic and genomic approaches reveal critical functions of H3K9 methylation and heterochromatin protein-1γ in reprogramming to pluripotency

Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) involves a marked reorganization of chromatin. To identify post-translational histone modifications that change in global abundance during this process, we have applied a quantitative mass-spectrometry-based approach. We f…