The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components
Open accessAbstract
To maintain genome function and stability, DNA sequence and its organization into chromatin must be duplicated during cell division. Understanding how entire chromosomes are copied remains a major challenge. Here, we use nascent chromatin capture (NCC) to profile chromatin proteome dynamics during replication in human cells. NCC relies on biotin–dUTP labelling of replicating DNA, affinity purification and quantitative proteomics. Comparing nascent chromatin with mature post-replicative chromatin, we provide association dynamics for 3,995 proteins. The replication machinery and 485 chromatin factors such as CAF-1, DNMT1 and SUV39h1 are enriched in nascent chromatin, whereas 170 factors including histone H1, DNMT3, MBD1-3 and PRC1 show delayed association. This correlates with H4K5K12diAc removal and H3K9me1 accumulation, whereas H3K27me3 and H3K9me3 remain unchanged. Finally, we combine NCC enrichment with experimentally derived chromatin probabilities to predict a function in nascent chromatin for 93 uncharacterized proteins, and identify FAM111A as a replication factor required for PCNA loading. Together, this provides an extensive resource to understand genome and epigenome maintenance.
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A decade of exploring the cancer epigenome—biological and translational implications
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Self-citationPCNA, the Maestro of the Replication Fork
Moldovan, G.-L., Pfander, B., & Jentsch, S. (2007) · Cell · doi:10.1016/j.cell.2007.05.003 · Cited by 1,796
Mechanisms and functions of DNA mismatch repair
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Role of the polycomb protein EED in the propagation of repressive histone marks
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Chromatin Compaction by a Polycomb Group Protein Complex
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Chromatin structure and the inheritance of epigenetic information
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The Metabolic Impact on Histone Acetylation and Transcription in Ageing
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Epigenome Maintenance in Response to DNA Damage
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Metadata
- Authors
- Alabert, C., Bukowski-Wills, J.-C., Lee, S.-B., Kustatscher, G., Nakamura, K., de Lima Alves, F., Menard, P., Mejlvang, J., Rappsilber, J., & Groth, A.
- Venue
- Nature Cell Biology
- Published
- 2014-02-23
- Volume
- 16
- Issue
- 3
- Pages
- 281-291
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2057130382
- Cited by
- 353
- References
- 53
- FWCI
- 14.72
- Type
- journal article
- Language
- en
- Open access
- green
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- Alabert, C., Bukowski-Wills, J.-C., Lee, S.-B., Kustatscher, G., Nakamura, K., de Lima Alves, F., Menard, P., Mejlvang, J., Rappsilber, J., & Groth, A. (2014). Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components. Nature Cell Biology. https://doi.org/10.1038/ncb2918
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