Cited by 862Cellular Mechanics and InteractionsCell Adhesion Molecules ResearchForce Microscopy Techniques and Applications

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Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity

Elosegui-Artola, Alberto, Oria, Roger, Chen, Yunfeng, Kosmalska, Anita, Pérez-González, Carlos, Castro, Natalia, Zhu, Cheng, Trepat, Xavier, Roca-Cusachs, Pere·2016·Nature Cell Biology

DOI 10.1038/ncb3336

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Abstract

Cell function depends on tissue rigidity, which cells probe by applying and transmitting forces to their extracellular matrix, and then transducing them into biochemical signals. Here we show that in response to matrix rigidity and density, force transmission and transduction are explained by the mechanical properties of the actin-talin-integrin-fibronectin clutch. We demonstrate that force transmission is regulated by a dynamic clutch mechanism, which unveils its fundamental biphasic force/rigidity relationship on talin depletion. Force transduction is triggered by talin unfolding above a stiffness threshold. Below this threshold, integrins unbind and release force before talin can unfold. Above the threshold, talin unfolds and binds to vinculin, leading to adhesion growth and YAP nuclear translocation. Matrix density, myosin contractility, integrin ligation and talin mechanical stability differently and nonlinearly regulate both force transmission and the transduction threshold. In all cases, coupling of talin unfolding dynamics to a theoretical clutch model quantitatively predicts cell response.

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Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Role of YAP/TAZ in mechanotransduction
Dupont, S., Morsut, L., Aragona, M., Enzo, E., Giulitti, S., Cordenonsi, M., Zanconato, F., Le Digabel, J., Forcato, M., Bicciato, S., et al. (2011) · Nature · doi:10.1038/nature10137 · Cited by 5,979
Calibration of atomic-force microscope tips
Hutter, J. L., & Bechhoefer, J. (1993) · Review of Scientific Instruments · doi:10.1063/1.1143970 · Cited by 4,205
Tensional homeostasis and the malignant phenotype
Paszek, M. J., Zahir, N., Johnson, K. R., Lakins, J. N., Rozenberg, G. I., Gefen, A., Reinhart-King, C. A., Margulies, S. S., Dembo, M., Boettiger, D., et al. (2005) · Cancer Cell · doi:10.1016/j.ccr.2005.08.010 · Cited by 3,941
Nuclear Lamin-A Scales with Tissue Stiffness and Enhances Matrix-Directed Differentiation
Swift, J., Ivanovska, I. L., Buxboim, A., Harada, T., Dingal, P. C. D. P., Pinter, J., Pajerowski, J. D., Spinler, K. R., Shin, J., Tewari, M., et al. (2013) · Science · doi:10.1126/science.1240104 · Cited by 1,998
Stretching Single Talin Rod Molecules Activates Vinculin Binding
Hernández, A. E. D. R., Pérez‐Jiménez, R., Liu, R., Roca‐Cusachs, P., Fernández, J. M., & Sheetz, M. P. (2009) · Science · doi:10.1126/science.1162912 · Cited by 1,492
Global Conformational Rearrangements in Integrin Extracellular Domains in Outside-In and Inside-Out Signaling
Takagi, J., Petre, B. M., Walz, T., & Springer, T. A. (2002) · Cell · doi:10.1016/s0092-8674(02)00935-2 · Cited by 1,168
Traction fields, moments, and strain energy that cells exert on their surroundings
Butler, J. P., Tolić, I. M., Fabry, B., & Fredberg, J. J. (2002) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.00270.2001 · Cited by 1,048
Balancing forces: architectural control of mechanotransduction
DuFort, C. C., Paszek, M. J., & Weaver, V. M. (2011) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3112 · Cited by 1,013
Traction Dynamics of Filopodia on Compliant Substrates
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BackgroundMethod
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Method
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Witte, K., Luo, J., Walker, M., Cantini, M., Seib, F. P., & Salmerón‐Sánchez, M. (2021) · Royal Society of Chemistry eBooks · doi:10.1039/9781839161124-00301 · Cited by 1
Mechanical Determinants of Tissue Development
Liaudanskaya, V., Sood, D., & Kaplan, D. L. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-809880-6.00024-2 · Cited by 4
Clutch model for focal adhesions predicts reduced self-stabilization under oblique pulling
Braeutigam, A., Burnet, A. F., Gompper, G., & Sabass, B. (2024) · Journal of Physics Condensed Matter · doi:10.1088/1361-648x/ad3ac1 · Cited by 1
“Cell adhesion models with such bond configurations are typically referred to as clutch models, see [57] and [5, 49, 56, 65, 66].”
BackgroundMethod
Cadherins and growth factor receptors – ligand-selective mechano-switches at cadherin junctions
Vu, V. H., Sullivan, B. G., Hebner, E., Rahil, Z., Zou, Y., & Leckband, D. E. (2025) · Journal of Cell Science · doi:10.1242/jcs.262279 · Cited by 1
“There are parallels between the actin clutch model used to describe integrin-mediated spreading (Chan and Odde, 2008; Elosegui-Artola et al., 2016) and intercellular adhesion (Noordstra et al., 2023).”
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Li, X., Konstantinou, R., Meena, V. K., Notash, S., Khalil, K., Whalley, T., Atherton, P., Barsukov, I., Zacharchenko, T., & Ballestrem, C. (2025) · The Journal of Cell Biology · doi:10.1083/jcb.202503155 · Cited by 1
“Since talin can be mechanically activated, we thought the increased retention of tensin3 could be due to talin changing its activation status when encountering substrates of different stiffness (Elosegui-Artola et al., 2016).”
Fine-tuning viscoelasticity: the key to collectively move in vivo
Espina, J. A., & Barriga, E. H. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-820310-1.00003-3 · Cited by 1
Biophysical origins of viscoelasticity during collective cell migration
Clark, A. G. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-820310-1.00007-0 · Cited by 1
Yap/Taz-TEAD ACTIVITY LINKS MECHANICAL CUES TO CELL PROGENITOR BEHAVIOR DURING HINDBRAIN SEGMENTATIONPreprint
Voltes, A., Hevia, C. F., Dingare, C., Calzolari, S., Terriente, J., Norden, C., Lecaudey, V., & Pujades, C. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/366351 · Cited by 1
A theoretical and computational study of soft adhesion mediated by mobile binders
Kaurin, D. (2018) · doi:10.5821/dissertation-2117-124704 · Cited by 1
Intercellular adhesion stiffness moderates cell decoupling on stiff substratesPreprint
Vargas, D. A., Heck, T., Smeets, B., Ramón, H., Parameswaran, H., & Oosterwyck, H. V. (2019) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/802520 · Cited by 1
“This is the first way in which mechanosensing is accounted for in this model, and it is based on findings showing that multi-protein adhesion complexes are capable of mechanosensing and are stabilized by force through protein recruitment (11, 18, 19).”
Background
The glycocalyx affects the mechanotransductive perception of the topographical microenvironmentPreprint
Chighizola, M., Dini, T., Marcotti, S., D’Urso, M., Piazzoni, C., Borghi, F., Previdi, A., Ceriani, L., Folliero, C., Stramer, B. M., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.03.02.433591 · Cited by 1
Does cellular adaptation to force loading rate determine the biphasic vs monotonic response of actin retrograde flow with substrate rigidity?Preprint
De, P. S., & De, R. (2021) · bioRxiv · doi:10.1101/2021.04.23.441062 · Cited by 1
“It is observed that the ligand-receptor binding affinity, unfolding the adapter proteins in the molecular clutch, and subsequent focal adhesions growth is modulated by the force loading rate [19].”
Background
Active superelasticity in three-dimensional epithelia of controlled shape
Ibars, E. L. (2021) · doi:10.5821/dissertation-2117-346402 · Cited by 1
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Franz, F., Tapia‐Rojo, R., Winograd-Katz, S. E., Boujemaa‐Paterski, R., Li, W., Unger, T., Albeck, S., Aponte-Santamaria, C. A., Garcia-Manyes, S., Medalia, O., et al. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.08.01.502287 · Cited by 1
New tools to study the interaction between integrins and latent TGFβ1Preprint
Bachmann, M., Kessler, J., Burri, E., & Wehrle‐Haller, B. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.01.26.525682 · Cited by 1
“Vinculin supports force transmission between the integrin-talin-actin axis (Elosegui-Artola et al., 2016; Humphries et al., 2007; Thievessen et al., 2013) and could be expected to facilitate αVβ6mediated mechanical TGFβ1 activation.”
Influential
Interactions between ploidy and resource availability shape clonal interference at initiation and recurrence of glioblastomaPreprint
Nowicka, Z., Rentzeperis, F., Beck, R. J., Tagal, V., Pinto, A. F., Scanu, E., Veith, T. A., Cole, J. P., Ilter, D., Dominguez‐Viqueira, W., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.17.562670 · Cited by 1
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Groeger, S. E., Wang, Y., Ruf, S., & Wang, L. (2023) · Research Square · doi:10.21203/rs.3.rs-3586140/v1 · Cited by 1
Weak and tunable adhesion-clutch drives rapid cell migration and glioblastoma motilityPreprint
Baba, K., Fukushi-Kumagaya, A., Morisaki, M., Takeuchi, R. F., Xiao, Z., Nagashima, Y., Sakai, M., Higashiguchi, Y., Katsuno‐Kambe, H., Katsuma, A., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.01.13.632873 · Cited by 1
Differential spatial regulation and activation of integrin nanoclusters inside focal adhesionsPreprint
Keary, S., Guevara-Garcia, A., Mateos, N., Campelo, F., & Parajo, M. F. G. (2025) · eLife · doi:10.7554/elife.105270 · Cited by 1
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Gandin, A., Torresan, V., Pelosin, M., Vanni, G., Busetto, R., Citron, A., Suli, A., Panciera, T., Zanconato, F., Piccolo, S., et al. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.06.04.656127 · Cited by 1
“The latter has been so far mainly conceptualized accordingly to a “molecular clutch” model, largely inferred from static cellular studies in cells experiencing substrates of fixed degrees of stiffness 36,37 .”
FCHo2, instead of talin, enables inside-out activation of integrin αvβ5 in curved adhesionsPreprint
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“Force-dependent activation of talin is a key regulatory step in the formation of focal adhesions, requiring a substrate rigidity >5 kPa 42 .”
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Sharma, U., Reber, J., Helenius, J. H., Buchholz, C., Faessler, R., Strohmeyer, N., & Müller, D. J. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2025.12.09.693059 · Cited by 1
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“The clutch model [46] has been instrumental in explaining and predicting cell adhesion behavior across various ECM conditions, including elastic [47, 48], viscoelastic [49, 50], and ligand density [51] (see [52] for a detailed overview).”
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DuChez, B. J. (2017) · ProQuest Demo Repository
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Roycroft, A. (2017) · UCL Discovery (University College London)
Phosphorylation of GMFγ by c-Abl coordinates lamellipodial and focal adhesion dynamicsPreprint
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Dependence of cellular behaviour on viscosity defined ligand mobility in supported lipid bilayers
Bennett, M. H. (2018) · Enlighten: Theses (The University of Glasgow)

Authors: Elosegui-Artola, A., Oria, R., Chen, Y., Kosmalska, A., Pérez-González, C., Castro, N., Zhu, C., Trepat, X., & Roca-Cusachs, P.
Affiliations: Institute for Bioengineering of Catalonia (ES)
Georgia Institute of Technology (US)
Institució Catalana de Recerca i Estudis Avançats (ES)
ORCID: Oria, Roger
Chen, Yunfeng
Zhu, Cheng
Trepat, Xavier
Roca-Cusachs, Pere
Venue: Nature Cell Biology
Publisher: Springer Science and Business Media LLC
Published: 2016-04-11
Volume: 18
Issue: 5
Pages: 540-548
ISSN: 1465-7392 (ISSN-L)
1476-4679
DOI: 10.1038/ncb3336
OpenAlex: W2327893020
Cited by: 858
References: 57
Citations by year: 2026: 72 · 2025: 101 · 2024: 87 · 2023: 93 · 2022: 103 · 2021: 93 · 2020: 96 · 2019: 71 · 2018: 67 · 2017: 60 · 2016: 15
FWCI: 48.78
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature Cell Biology (publishedVersion)
PubMed (publishedVersion)
Dipòsit Digital de la Universitat de Barcelona (Universitat de Barcelona) (submittedVersion, public-domain)
Funders: National Institute of Allergy and Infectious Diseases (R01AI044902)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
Keywords: molecular clutch, matrix rigidity, force transmission, mechanotransduction, vinculin, YAP nuclear translocation, cell adhesion, extracellular matrix stiffness, myosin contractility, actin cytoskeleton, fibronectin
MeSH terms: YAP-Signaling Proteins, Animals, Biomechanical Phenomena, Cell Adhesion, Cell Nucleus, Cytoskeleton, Extracellular Matrix, Fibronectins, Phosphoproteins, Phosphorylation, Protein Binding, Integrins, Vinculin, Talin, Cell Cycle Proteins, Protein Transport, Stress Fibers, Mechanotransduction, Cellular, Adaptor Proteins, Signal Transducing, Mice, Focal Adhesion Protein-Tyrosine Kinases

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Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity

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