The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
Programmable autonomous synthesis of single-stranded DNA
Open accessAbstract
DNA performs diverse functional roles in biology, nanotechnology, and biotechnology, but current methods for autonomously synthesizing arbitrary single-stranded DNA are limited. Here, we introduce the concept of Primer Exchange Reaction (PER) cascades, which grow nascent single-stranded DNA with user-specified sequences following prescribed reaction pathways. PER synthesis happens in a programmable, autonomous, in situ, and environmentally responsive fashion, providing a platform for engineering molecular circuits and devices with a wide range of sensing, monitoring, recording, signal processing, and actuation capabilities. We experimentally demonstrate a nanodevice that transduces the detection of a trigger RNA into the production of a DNAzyme that degrades an independent RNA substrate, a signal amplifier that conditionally synthesizes long fluorescent strands only in the presence of a particular RNA signal, molecular computing circuits that evaluate logic (AND, OR, NOT) combinations of RNA inputs, and a temporal molecular event recorder that records in the PER transcript the order in which distinct RNA inputs are sequentially detected.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
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“Note that the copy region can be much longer than the primer binding region, which is typically kept at 7–9 nt under our typical reaction conditions to simultaneously allow (1) efficient primer binding and priming for polymerization and (2) dissociation of the elongated transcript from the hairpin.”
BackgroundSelf-assembly of DNA into nanoscale three-dimensional shapes
Douglas, S. M., Dietz, H., Liedl, T., Högberg, B., Graf, F., & Shih, W. M. (2009) · Nature · doi:10.1038/nature08016 · Cited by 2,571
A DNA-fuelled molecular machine made of DNA
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DNA-based self-assembly of chiral plasmonic nanostructures with tailored optical response
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A Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads
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Triggered amplification by hybridization chain reaction
Dirks, R. M., & Pierce, N. A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0407024101 · Cited by 2,063
Dynamic DNA nanotechnology using strand-displacement reactions
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Cited by
Highly multiplexed in situ protein imaging with signal amplification by Immuno-SABER
Saka, S. K., Wang, Y., Kishi, J. Y., Zhu, A., Zeng, Y., Xie, W., Kirli, K., Yapp, C., Cicconet, M., Beliveau, B. J., et al. (2018) · doi:10.1101/507566 · Cited by 485
Self-citationImmuno-SABER enables highly multiplexed and amplified protein imaging in tissues
Saka, S. K., Wang, Y., Kishi, J. Y., Zhu, A., Zeng, Y., Xie, W., Kirli, K., Yapp, C., Cicconet, M., Beliveau, B. J., et al. (2019) · Nature Biotechnology · doi:10.1038/s41587-019-0207-y · Cited by 481
Self-citationSABER amplifies FISH: enhanced multiplexed imaging of RNA and DNA in cells and tissues
Kishi, J. Y., Lapan, S. W., Beliveau, B. J., West, E. R., Zhu, A., Sasaki, H. M., Saka, S. K., Wang, Y., Cepko, C. L., & Yin, P. (2019) · Nature Methods · doi:10.1038/s41592-019-0404-0 · Cited by 454
Self-citationAAV cis -regulatory sequences are correlated with ocular toxicity
Xiong, W., Wu, D. M., Xue, Y., Wang, S. K., Chung, M. J., Ji, X., Rana, P., Zhao, S. R., Mai, S., & Cepko, C. L. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1821000116 · Cited by 249
Building machines with DNA molecules
Ramezani, H., & Dietz, H. (2020) · Nature Reviews Genetics · doi:10.1038/s41576-019-0175-6 · Cited by 284
Metadata
- Authors
- Kishi, J. Y., Schaus, T. E., Gopalkrishnan, N., Xuan, F., & Yin, P.
- Venue
- Nature Chemistry
- Published
- 2017-11-06
- Volume
- 10
- Issue
- 2
- Pages
- 155-164
- ISSN
- 1755-4330 (ISSN-L)
- 1755-4349
- OpenAlex
- W2767343818
- Cited by
- 316
- References
- 62
- FWCI
- 5.82
- Type
- journal article
- Language
- en
- Open access
- green
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