The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
PI(3,5)P2 controls membrane trafficking by direct activation of mucolipin Ca2+ release channels in the endolysosome
Open accessAbstract
Membrane fusion and fission in intracellular trafficking is controlled by both intraluminal Ca2+ release and phosphoinositide signaling. However, the molecular identities of the Ca2+ release channels and the target proteins of phosphoinositides are elusive. Here, by direct patch-clamping of the endolysosomal membrane, we report that PI(3,5)P2, an endolysosome-specific phosphoinositide, binds and activates endolysosome-localized mucolipin TRP (TRPML) channels with specificity and potency. Both PI(3,5)P2-deficient cells and cells that lack TRPML1 exhibited enlarged endolysosomes/vacuoles and trafficking defects in the late endocytic pathway. We find that the enlarged vacuole phenotype observed in PI(3,5)P2-deficient mouse fibroblasts is suppressed by overexpression of TRPML1. Notably, this PI(3,5)P2-dependent regulation of TRPML1 is evolutionarily conserved. In budding yeast, hyperosmotic stress induces Ca2+ release from the vacuole. Here, we show that this release requires both PI(3,5)P2 production and a yeast functional TRPML homolog. We propose that TRPMLs regulate membrane trafficking by transducing information about PI(3,5)P2 levels into changes in juxtaorganellar Ca2+, thereby triggering membrane fusion/fission events.
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Cites
NAADP mobilizes calcium from acidic organelles through two-pore channels
Calcraft, P. J., Ruas, M., Pan, Z., Cheng, X., Arredouani, A., Hao, X., Tang, J., Rietdorf, K., Teboul, L., Chuang, K.-T., et al. (2009) · Nature · doi:10.1038/nature08030 · Cited by 760
Self-citationPIP 2 Is a Necessary Cofactor for Ion Channel Function: How and Why?
Suh, B.-C., & Hille, B. (2008) · Annual Review of Biophysics · doi:10.1146/annurev.biophys.37.032807.125859 · Cited by 646
Defective autophagy in neurons and astrocytes from mice deficient in PI(3,5)P2
Ferguson, C. J., Lenk, G. M., & Meisler, M. H. (2009) · Human Molecular Genetics · doi:10.1093/hmg/ddp460 · Cited by 202
Cited by
Phosphoinositides: Tiny Lipids With Giant Impact on Cell Regulation
Balla, T. (2013) · Physiological Reviews · doi:10.1152/physrev.00028.2012 · Cited by 1,638
Lysosomes as dynamic regulators of cell and organismal homeostasis
Ballabio, A., & Bonifacino, J. S. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0185-4 · Cited by 1,469
MCOLN1 is a ROS sensor in lysosomes that regulates autophagy
Zhang, X., Cheng, X., Yu, L., Yang, J., Calvo, R., Patnaik, S., Hu, X., Gao, Q., Yang, M., Lawas, M., et al. (2016) · Nature Communications · doi:10.1038/ncomms12109 · Cited by 558
Self-citationLiao, Y.-C., Fernandopulle, M. S., Wang, G., Choi, H., Hao, L., Drerup, C. M., Patel, R., Qamar, S., Nixon-Abell, J., Shen, Y., et al. (2019) · Cell · doi:10.1016/j.cell.2019.08.050 · Cited by 550
Phosphoinositides as membrane organizers
Posor, Y., Jang, W., & Haucke, V. (2022) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-022-00490-x · Cited by 469
Metadata
- Authors
- Dong, X.-P., Shen, D., Wang, X., Dawson, T., Li, X., Zhang, Q., Cheng, X., Zhang, Y., Weisman, L. S., Delling, M., & Xu, H.
- Venue
- Nature Communications
- Published
- 2010-07-13
- Volume
- 1
- Issue
- 1
- Pages
- 38-38
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W1993401680
- Cited by
- 635
- References
- 3
- FWCI
- 15.87
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Dong, X.-P., Shen, D., Wang, X., Dawson, T., Li, X., Zhang, Q., Cheng, X., Zhang, Y., Weisman, L. S., Delling, M., & Xu, H. (2010). PI(3,5)P2 controls membrane trafficking by direct activation of mucolipin Ca2+ release channels in the endolysosome. Nature Communications. https://doi.org/10.1038/ncomms1037
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